Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light sca...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/11/11/416 |
| _version_ | 1797511048372158464 |
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| author | Zhihang Xiong Hongjian Wang Jiajin Li Ran Liao Haoji Mai Caizhong Guan Zhiming Guo Shangpan Yang Yan Chen Biwang Liu Tong Liu Hongyi Li Wenzheng Ding Yaguang Zeng Hui Ma |
| author_facet | Zhihang Xiong Hongjian Wang Jiajin Li Ran Liao Haoji Mai Caizhong Guan Zhiming Guo Shangpan Yang Yan Chen Biwang Liu Tong Liu Hongyi Li Wenzheng Ding Yaguang Zeng Hui Ma |
| author_sort | Zhihang Xiong |
| collection | DOAJ |
| description | Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of <i>Synechococcus</i>. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring. |
| first_indexed | 2024-03-10T05:39:54Z |
| format | Article |
| id | doaj.art-0448b7c2bb0e4a6e8f19bf92da691eaf |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T05:39:54Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-0448b7c2bb0e4a6e8f19bf92da691eaf2023-11-22T22:35:50ZengMDPI AGBiosensors2079-63742021-10-01111141610.3390/bios11110416Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and FluorescenceZhihang Xiong0Hongjian Wang1Jiajin Li2Ran Liao3Haoji Mai4Caizhong Guan5Zhiming Guo6Shangpan Yang7Yan Chen8Biwang Liu9Tong Liu10Hongyi Li11Wenzheng Ding12Yaguang Zeng13Hui Ma14Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaInstitute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaInstitute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaInstitute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaDepartment of Photoelectric Technology, Foshan University, Foshan 528000, ChinaDepartment of Photoelectric Technology, Foshan University, Foshan 528000, ChinaInstitute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaDepartment of Photoelectric Technology, Foshan University, Foshan 528000, ChinaInstitute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaDepartment of Photoelectric Technology, Foshan University, Foshan 528000, ChinaInstitute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaDepartment of Photoelectric Technology, Foshan University, Foshan 528000, ChinaDepartment of Photoelectric Technology, Foshan University, Foshan 528000, ChinaDepartment of Photoelectric Technology, Foshan University, Foshan 528000, ChinaGuangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaSuspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of <i>Synechococcus</i>. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring.https://www.mdpi.com/2079-6374/11/11/416polarized light scatteringfluorescencesuspended particles |
| spellingShingle | Zhihang Xiong Hongjian Wang Jiajin Li Ran Liao Haoji Mai Caizhong Guan Zhiming Guo Shangpan Yang Yan Chen Biwang Liu Tong Liu Hongyi Li Wenzheng Ding Yaguang Zeng Hui Ma Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence Biosensors polarized light scattering fluorescence suspended particles |
| title | Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence |
| title_full | Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence |
| title_fullStr | Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence |
| title_full_unstemmed | Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence |
| title_short | Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence |
| title_sort | probing individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence |
| topic | polarized light scattering fluorescence suspended particles |
| url | https://www.mdpi.com/2079-6374/11/11/416 |
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