Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran
Background: Female sand flies of subgenus Adlerius are considered as probable vectors of visceral leishmaniasis in Iran. The objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country. Methods: Sand flies collected using sti...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-10-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/120 |
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| author | Alireza Zahraei-Ramazani Dinesh Kumar Hossein Mirhendi Shyam Sundar Rajnikan Mishra Vahideh Moin-Vaziri Hassan Soleimani MohammadReza Shirzadi Reza Jafari AhmadAli Hanafi-Bojd Sodabe Hamedi-Shahraky MohammadReza Yaghoobi-Ershadi |
| author_facet | Alireza Zahraei-Ramazani Dinesh Kumar Hossein Mirhendi Shyam Sundar Rajnikan Mishra Vahideh Moin-Vaziri Hassan Soleimani MohammadReza Shirzadi Reza Jafari AhmadAli Hanafi-Bojd Sodabe Hamedi-Shahraky MohammadReza Yaghoobi-Ershadi |
| author_sort | Alireza Zahraei-Ramazani |
| collection | DOAJ |
| description | Background: Female sand flies of subgenus Adlerius are considered as probable vectors of visceral leishmaniasis in Iran. The objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country.
Methods: Sand flies collected using sticky traps from 17 provinces during 2008–2010. The morphometric measurements were conducted with an Ocular Micrometer. Data was analyzed by SPSS. The Cytb gene was used to estimate population genetic diversity and identify the female specimens. UPGMA phenetic tree was used for DNA haplotypes of Cytb gene.
Results: Six species of subgenus Adlerius identified from which one species, P. (Adlerius) kabulensis, is new record. The identification key is provided for males. Results revealed the molecular systematic in the species of subgenus Adlerius and determine the relationship of three females of P. comatus, P. balcanicus and P. halepensis.
Conclusion: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results. The branches of each species are not genetically distinct which justify the overlapping morphological characters among them. Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran. |
| first_indexed | 2024-12-18T19:26:35Z |
| format | Article |
| id | doaj.art-04491439a305470ba335f336d7013491 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-18T19:26:35Z |
| publishDate | 2015-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-04491439a305470ba335f336d70134912022-12-21T20:55:50ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712015-10-0191108Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in IranAlireza Zahraei-Ramazani0Dinesh Kumar1Hossein Mirhendi2Shyam Sundar3Rajnikan Mishra4Vahideh Moin-Vaziri5Hassan Soleimani6MohammadReza Shirzadi7Reza Jafari8AhmadAli Hanafi-Bojd9Sodabe Hamedi-Shahraky10MohammadReza Yaghoobi-Ershadi11Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.Department of Zoology, Centre of Advanced Study, Banaras Hindu University, Varanasi 221 005, India.Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India.Department of Zoology, Centre of Advanced Study, Banaras Hindu University, Varanasi 221 005, India.Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Yazd Health Research Station, National Institute of Health Research, Yazd, Iran.Communicable Disease Management Centre, Ministry of Health and Medical Education, Tehran, Iran.Esfahan Health Research Station, National Institute of Health Research, Esfahan, Iran.Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.School of Health, Zabol University of Medical Sciences, Zabol, Iran.Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.Background: Female sand flies of subgenus Adlerius are considered as probable vectors of visceral leishmaniasis in Iran. The objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country. Methods: Sand flies collected using sticky traps from 17 provinces during 2008–2010. The morphometric measurements were conducted with an Ocular Micrometer. Data was analyzed by SPSS. The Cytb gene was used to estimate population genetic diversity and identify the female specimens. UPGMA phenetic tree was used for DNA haplotypes of Cytb gene. Results: Six species of subgenus Adlerius identified from which one species, P. (Adlerius) kabulensis, is new record. The identification key is provided for males. Results revealed the molecular systematic in the species of subgenus Adlerius and determine the relationship of three females of P. comatus, P. balcanicus and P. halepensis. Conclusion: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results. The branches of each species are not genetically distinct which justify the overlapping morphological characters among them. Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran.https://jad.tums.ac.ir/index.php/jad/article/view/120Phlebotomine sand fliessubgenus AdleriusP. comatusP. kabulensisMitochondrial DNA Cytochrome b gene |
| spellingShingle | Alireza Zahraei-Ramazani Dinesh Kumar Hossein Mirhendi Shyam Sundar Rajnikan Mishra Vahideh Moin-Vaziri Hassan Soleimani MohammadReza Shirzadi Reza Jafari AhmadAli Hanafi-Bojd Sodabe Hamedi-Shahraky MohammadReza Yaghoobi-Ershadi Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran Journal of Arthropod-Borne Diseases Phlebotomine sand flies subgenus Adlerius P. comatus P. kabulensis Mitochondrial DNA Cytochrome b gene |
| title | Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran |
| title_full | Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran |
| title_fullStr | Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran |
| title_full_unstemmed | Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran |
| title_short | Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran |
| title_sort | morphological and genotypic variations among the species of the subgenus adlerius diptera psychodidae phlebotomus in iran |
| topic | Phlebotomine sand flies subgenus Adlerius P. comatus P. kabulensis Mitochondrial DNA Cytochrome b gene |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/120 |
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