Quantitative isolation of total glycosphingolipids from animal cells
The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a m...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
1971-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520395377 |
| _version_ | 1818716101647794176 |
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| author | Terunobu Saito Sen-itiroh Hakomori |
| author_facet | Terunobu Saito Sen-itiroh Hakomori |
| author_sort | Terunobu Saito |
| collection | DOAJ |
| description | The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia–silica gel (Florisil) column, and (c) deacetylation of glycolipid in chloroform–methanol–sodium methoxide. This method is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells. |
| first_indexed | 2024-12-17T19:13:54Z |
| format | Article |
| id | doaj.art-044c9da490c54387b6773782406511e1 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T19:13:54Z |
| publishDate | 1971-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-044c9da490c54387b6773782406511e12022-12-21T21:35:47ZengElsevierJournal of Lipid Research0022-22751971-03-01122257259Quantitative isolation of total glycosphingolipids from animal cellsTerunobu Saito0Sen-itiroh Hakomori1Biochemical Laboratory, Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98105Biochemical Laboratory, Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98105The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia–silica gel (Florisil) column, and (c) deacetylation of glycolipid in chloroform–methanol–sodium methoxide. This method is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells.http://www.sciencedirect.com/science/article/pii/S0022227520395377glycolipidsacetylationacetic anhydridepyridinemagnesia–silica gel (Florisil)1,2-dichloroethane (DCE) |
| spellingShingle | Terunobu Saito Sen-itiroh Hakomori Quantitative isolation of total glycosphingolipids from animal cells Journal of Lipid Research glycolipids acetylation acetic anhydride pyridine magnesia–silica gel (Florisil) 1,2-dichloroethane (DCE) |
| title | Quantitative isolation of total glycosphingolipids from animal cells |
| title_full | Quantitative isolation of total glycosphingolipids from animal cells |
| title_fullStr | Quantitative isolation of total glycosphingolipids from animal cells |
| title_full_unstemmed | Quantitative isolation of total glycosphingolipids from animal cells |
| title_short | Quantitative isolation of total glycosphingolipids from animal cells |
| title_sort | quantitative isolation of total glycosphingolipids from animal cells |
| topic | glycolipids acetylation acetic anhydride pyridine magnesia–silica gel (Florisil) 1,2-dichloroethane (DCE) |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520395377 |
| work_keys_str_mv | AT terunobusaito quantitativeisolationoftotalglycosphingolipidsfromanimalcells AT senitirohhakomori quantitativeisolationoftotalglycosphingolipidsfromanimalcells |