Quantitative isolation of total glycosphingolipids from animal cells
The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a m...
Main Authors: | Terunobu Saito, Sen-itiroh Hakomori |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1971-03-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520395377 |
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