Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions
Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images i...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/8/4053 |
| _version_ | 1797537629267296256 |
|---|---|
| author | Ewa Bączyńska Katarzyna Karolina Pels Subhadip Basu Jakub Włodarczyk Błażej Ruszczycki |
| author_facet | Ewa Bączyńska Katarzyna Karolina Pels Subhadip Basu Jakub Włodarczyk Błażej Ruszczycki |
| author_sort | Ewa Bączyńska |
| collection | DOAJ |
| description | Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images is one of the key elements towards understanding mechanisms of structural neuronal plasticity and bases of brain pathology. In the following article, we review experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. We compare various methodological pipelines of biological models, sample preparation, data analysis, image acquisition, sample size, and statistical analysis. The methodology and results of relevant experiments are systematically summarized in a tabular form. In particular, we focus on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance. |
| first_indexed | 2024-03-10T12:19:10Z |
| format | Article |
| id | doaj.art-044db47bfff74a84a769f9cd9bf1ddcd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:19:10Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-044db47bfff74a84a769f9cd9bf1ddcd2023-11-21T15:36:01ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01228405310.3390/ijms22084053Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological ConditionsEwa Bączyńska0Katarzyna Karolina Pels1Subhadip Basu2Jakub Włodarczyk3Błażej Ruszczycki4Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, PolandNencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, PolandDepartment of Computer Science and Engineering, Jadvapur University, Kolkata 700032, IndiaNencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, PolandNencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, PolandNumerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images is one of the key elements towards understanding mechanisms of structural neuronal plasticity and bases of brain pathology. In the following article, we review experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. We compare various methodological pipelines of biological models, sample preparation, data analysis, image acquisition, sample size, and statistical analysis. The methodology and results of relevant experiments are systematically summarized in a tabular form. In particular, we focus on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance.https://www.mdpi.com/1422-0067/22/8/4053dendritic spinesdendritic spine analysisspine remodelingsynaptic plasticitystructural plasticityneuronal remodeling |
| spellingShingle | Ewa Bączyńska Katarzyna Karolina Pels Subhadip Basu Jakub Włodarczyk Błażej Ruszczycki Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions International Journal of Molecular Sciences dendritic spines dendritic spine analysis spine remodeling synaptic plasticity structural plasticity neuronal remodeling |
| title | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
| title_full | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
| title_fullStr | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
| title_full_unstemmed | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
| title_short | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
| title_sort | quantification of dendritic spines remodeling under physiological stimuli and in pathological conditions |
| topic | dendritic spines dendritic spine analysis spine remodeling synaptic plasticity structural plasticity neuronal remodeling |
| url | https://www.mdpi.com/1422-0067/22/8/4053 |
| work_keys_str_mv | AT ewabaczynska quantificationofdendriticspinesremodelingunderphysiologicalstimuliandinpathologicalconditions AT katarzynakarolinapels quantificationofdendriticspinesremodelingunderphysiologicalstimuliandinpathologicalconditions AT subhadipbasu quantificationofdendriticspinesremodelingunderphysiologicalstimuliandinpathologicalconditions AT jakubwłodarczyk quantificationofdendriticspinesremodelingunderphysiologicalstimuliandinpathologicalconditions AT błazejruszczycki quantificationofdendriticspinesremodelingunderphysiologicalstimuliandinpathologicalconditions |