Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities
The diamond back moth, <i>Plutella xylostella</i>, causes severe damage at all crop stages, beside its rising resistance to all insecticides. The objective of this study was to look for a new control strategy such as application of insecticide-loaded carbon dot-embedded fluorescent mesop...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| author | Muhammad Bilal Muhammad Umair Sial Lidong Cao Qiliang Huang |
| author_facet | Muhammad Bilal Muhammad Umair Sial Lidong Cao Qiliang Huang |
| author_sort | Muhammad Bilal |
| collection | DOAJ |
| description | The diamond back moth, <i>Plutella xylostella</i>, causes severe damage at all crop stages, beside its rising resistance to all insecticides. The objective of this study was to look for a new control strategy such as application of insecticide-loaded carbon dot-embedded fluorescent mesoporous silica nanoparticles (FL-SiO<sub>2</sub> NPs). Two different-sized methoxyfenozide-loaded nanoparticles (Me@FL-SiO<sub>2</sub> NPs-70 nm, Me@FL-SiO<sub>2</sub> NPs-150 nm) were prepared, with loading content 15% and 16%. Methoxyfenozide was released constantly from Me@FL-SiO<sub>2</sub> NPs only at specific optimum pH 7.5. The release of methoxyfenozide from Me@FL-SiO<sub>2</sub> NPs was not observed other than this optimum pH, and therefore, we checked and controlled a single release condition to look out for the different particle sizes of insecticide-loaded NPs. This pH-responsive release pattern can find potential application in sustainable plant protection. Moreover, the lethal concentration of the LC<sub>50</sub> value was 24 mg/L for methoxyfenozide (TC), 14 mg/L for Me@FL-SiO<sub>2</sub> NPs-70 nm, and 15 mg/L for Me@FL-SiO<sub>2</sub> NPs-150 nm after 72 h exposure, respectively. After calculating the LC<sub>50</sub>, the results predicted that Me@FL-SiO<sub>2</sub> NPs-70 nm and Me@FL-SiO<sub>2</sub> NPs-150 nm exhibited better insecticidal activity against <i>P. xylostella</i> than methoxyfenozide under the same concentrations of active ingredient applied. Moreover, the activities of detoxification enzymes of <i>P. xylostella</i> were suppressed by treatment with insecticide-loaded NPs, which showed that NPs could also be involved in reduction of enzymes. Furthermore, the entering of FL-SiO<sub>2</sub> NPs into the midgut of <i>P. xylostella</i> was confirmed by confocal laser scanning microscope (CLSM). For comparison, <i>P. xylostella</i> under treatment with water as control was also observed under CLSM. The control exhibited no fluorescent signal, while the larvae treated with FL-SiO<sub>2</sub> NPs showed strong fluorescence under a laser excitation wavelength of 448 nm. The reduced enzyme activities as well as higher cuticular penetration in insects indicate that the nano-based delivery system of insecticide could be potentially applied in insecticide resistance management. |
| first_indexed | 2024-03-10T03:42:07Z |
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| spelling | doaj.art-044e421740014cd29e99a6a395fdc4872023-11-23T11:28:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310579010.3390/ijms23105790Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels ActivitiesMuhammad Bilal0Muhammad Umair Sial1Lidong Cao2Qiliang Huang3Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaDepartment of Entomology, University of Agriculture, Faisalabad 38000, PakistanInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaThe diamond back moth, <i>Plutella xylostella</i>, causes severe damage at all crop stages, beside its rising resistance to all insecticides. The objective of this study was to look for a new control strategy such as application of insecticide-loaded carbon dot-embedded fluorescent mesoporous silica nanoparticles (FL-SiO<sub>2</sub> NPs). Two different-sized methoxyfenozide-loaded nanoparticles (Me@FL-SiO<sub>2</sub> NPs-70 nm, Me@FL-SiO<sub>2</sub> NPs-150 nm) were prepared, with loading content 15% and 16%. Methoxyfenozide was released constantly from Me@FL-SiO<sub>2</sub> NPs only at specific optimum pH 7.5. The release of methoxyfenozide from Me@FL-SiO<sub>2</sub> NPs was not observed other than this optimum pH, and therefore, we checked and controlled a single release condition to look out for the different particle sizes of insecticide-loaded NPs. This pH-responsive release pattern can find potential application in sustainable plant protection. Moreover, the lethal concentration of the LC<sub>50</sub> value was 24 mg/L for methoxyfenozide (TC), 14 mg/L for Me@FL-SiO<sub>2</sub> NPs-70 nm, and 15 mg/L for Me@FL-SiO<sub>2</sub> NPs-150 nm after 72 h exposure, respectively. After calculating the LC<sub>50</sub>, the results predicted that Me@FL-SiO<sub>2</sub> NPs-70 nm and Me@FL-SiO<sub>2</sub> NPs-150 nm exhibited better insecticidal activity against <i>P. xylostella</i> than methoxyfenozide under the same concentrations of active ingredient applied. Moreover, the activities of detoxification enzymes of <i>P. xylostella</i> were suppressed by treatment with insecticide-loaded NPs, which showed that NPs could also be involved in reduction of enzymes. Furthermore, the entering of FL-SiO<sub>2</sub> NPs into the midgut of <i>P. xylostella</i> was confirmed by confocal laser scanning microscope (CLSM). For comparison, <i>P. xylostella</i> under treatment with water as control was also observed under CLSM. The control exhibited no fluorescent signal, while the larvae treated with FL-SiO<sub>2</sub> NPs showed strong fluorescence under a laser excitation wavelength of 448 nm. The reduced enzyme activities as well as higher cuticular penetration in insects indicate that the nano-based delivery system of insecticide could be potentially applied in insecticide resistance management.https://www.mdpi.com/1422-0067/23/10/5790<i>Plutella xylostella</i>nanotechnologyfluorescent silica nanoparticlesdetoxification enzymesinsecticide resistance |
| spellingShingle | Muhammad Bilal Muhammad Umair Sial Lidong Cao Qiliang Huang Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities International Journal of Molecular Sciences <i>Plutella xylostella</i> nanotechnology fluorescent silica nanoparticles detoxification enzymes insecticide resistance |
| title | Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities |
| title_full | Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities |
| title_fullStr | Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities |
| title_full_unstemmed | Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities |
| title_short | Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities |
| title_sort | effects of methoxyfenozide loaded fluorescent mesoporous silica nanoparticles on i plutella xylostella i l lepidoptera plutellidae mortality and detoxification enzyme levels activities |
| topic | <i>Plutella xylostella</i> nanotechnology fluorescent silica nanoparticles detoxification enzymes insecticide resistance |
| url | https://www.mdpi.com/1422-0067/23/10/5790 |
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