Bioremediation by <i>Cupriavidus metallidurans</i> Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle Microorganisms
Nitrogen cycle microorganisms are essential in agricultural soils and may be affected by mercury pollution. The aims of this study are to evaluate the bioremediation of mercury-polluted agricultural soil using <i>Cupriavidus metallidurans</i> MSR33 in a rotary drum bioreactor (RDB) and t...
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/1952 |
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| author | Guillermo Bravo Paulina Vega-Celedón Juan Carlos Gentina Michael Seeger |
| author_facet | Guillermo Bravo Paulina Vega-Celedón Juan Carlos Gentina Michael Seeger |
| author_sort | Guillermo Bravo |
| collection | DOAJ |
| description | Nitrogen cycle microorganisms are essential in agricultural soils and may be affected by mercury pollution. The aims of this study are to evaluate the bioremediation of mercury-polluted agricultural soil using <i>Cupriavidus metallidurans</i> MSR33 in a rotary drum bioreactor (RDB) and to characterize the effects of mercury pollution and bioremediation on nitrogen cycle microorganisms. An agricultural soil was contaminated with mercury (II) (20–30 ppm) and subjected to bioremediation using strain MSR33 in a custom-made RDB. The effects of mercury and bioremediation on nitrogen cycle microorganisms were studied by qPCR. Bioremediation in the RDB removed 82% mercury. MSR33 cell concentrations, thioglycolate, and mercury concentrations influence mercury removal. Mercury pollution strongly decreased nitrogen-fixing and nitrifying bacterial communities in agricultural soils. Notably, after soil bioremediation process nitrogen-fixing and nitrifying bacteria significantly increased. Diverse mercury-tolerant strains were isolated from the bioremediated soil. The isolates <i>Glutamicibacter</i> sp. SB1a, <i>Brevundimonas</i> sp. SB3b, and <i>Ochrobactrum</i> sp. SB4b possessed the <i>merG</i> gene associated with the plasmid pTP6, suggesting the horizontal transfer of this plasmid to native gram-positive and gram-negative bacteria. Bioremediation by strain MSR33 in an RDB is an attractive and innovative technology for the clean-up of mercury-polluted agricultural soils and the recovery of nitrogen cycle microbial communities. |
| first_indexed | 2024-03-10T14:13:15Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-10T14:13:15Z |
| publishDate | 2020-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-044fb82fa0e24a0a80c1a8361955d15c2023-11-20T23:59:55ZengMDPI AGMicroorganisms2076-26072020-12-01812195210.3390/microorganisms8121952Bioremediation by <i>Cupriavidus metallidurans</i> Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle MicroorganismsGuillermo Bravo0Paulina Vega-Celedón1Juan Carlos Gentina2Michael Seeger3Molecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry & Center of Biotechnology Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileMolecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry & Center of Biotechnology Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileSchool of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso 2362803, ChileMolecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry & Center of Biotechnology Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileNitrogen cycle microorganisms are essential in agricultural soils and may be affected by mercury pollution. The aims of this study are to evaluate the bioremediation of mercury-polluted agricultural soil using <i>Cupriavidus metallidurans</i> MSR33 in a rotary drum bioreactor (RDB) and to characterize the effects of mercury pollution and bioremediation on nitrogen cycle microorganisms. An agricultural soil was contaminated with mercury (II) (20–30 ppm) and subjected to bioremediation using strain MSR33 in a custom-made RDB. The effects of mercury and bioremediation on nitrogen cycle microorganisms were studied by qPCR. Bioremediation in the RDB removed 82% mercury. MSR33 cell concentrations, thioglycolate, and mercury concentrations influence mercury removal. Mercury pollution strongly decreased nitrogen-fixing and nitrifying bacterial communities in agricultural soils. Notably, after soil bioremediation process nitrogen-fixing and nitrifying bacteria significantly increased. Diverse mercury-tolerant strains were isolated from the bioremediated soil. The isolates <i>Glutamicibacter</i> sp. SB1a, <i>Brevundimonas</i> sp. SB3b, and <i>Ochrobactrum</i> sp. SB4b possessed the <i>merG</i> gene associated with the plasmid pTP6, suggesting the horizontal transfer of this plasmid to native gram-positive and gram-negative bacteria. Bioremediation by strain MSR33 in an RDB is an attractive and innovative technology for the clean-up of mercury-polluted agricultural soils and the recovery of nitrogen cycle microbial communities.https://www.mdpi.com/2076-2607/8/12/1952<i>Cupriavidus metallidurans</i>mercurynitrogen cyclerotary drum bioreactorsoil bioremediation |
| spellingShingle | Guillermo Bravo Paulina Vega-Celedón Juan Carlos Gentina Michael Seeger Bioremediation by <i>Cupriavidus metallidurans</i> Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle Microorganisms Microorganisms <i>Cupriavidus metallidurans</i> mercury nitrogen cycle rotary drum bioreactor soil bioremediation |
| title | Bioremediation by <i>Cupriavidus metallidurans</i> Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle Microorganisms |
| title_full | Bioremediation by <i>Cupriavidus metallidurans</i> Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle Microorganisms |
| title_fullStr | Bioremediation by <i>Cupriavidus metallidurans</i> Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle Microorganisms |
| title_full_unstemmed | Bioremediation by <i>Cupriavidus metallidurans</i> Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle Microorganisms |
| title_short | Bioremediation by <i>Cupriavidus metallidurans</i> Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle Microorganisms |
| title_sort | bioremediation by i cupriavidus metallidurans i strain msr33 of mercury polluted agricultural soil in a rotary drum bioreactor and its effects on nitrogen cycle microorganisms |
| topic | <i>Cupriavidus metallidurans</i> mercury nitrogen cycle rotary drum bioreactor soil bioremediation |
| url | https://www.mdpi.com/2076-2607/8/12/1952 |
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