High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus)
Recently, the prevalence of hypertension has become a global challenge. Therefore, tremendous efforts have been made to identify and purify antihypertensive peptides (AHTPs) from food-derived proteins to aid the discovery of new hypotensive drugs with fewer side effects. In this study, we performed...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.863310/full |
| _version_ | 1817976016487841792 |
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| author | Yu Huang Xiyang Chen Haoyue Shu Panpan Xiao Xueqiang Lin Junmin Xu Chao Bian Chao Bian Xinxin You Xinxin You Jian Yang Qiong Shi Qiong Shi Qiong Shi |
| author_facet | Yu Huang Xiyang Chen Haoyue Shu Panpan Xiao Xueqiang Lin Junmin Xu Chao Bian Chao Bian Xinxin You Xinxin You Jian Yang Qiong Shi Qiong Shi Qiong Shi |
| author_sort | Yu Huang |
| collection | DOAJ |
| description | Recently, the prevalence of hypertension has become a global challenge. Therefore, tremendous efforts have been made to identify and purify antihypertensive peptides (AHTPs) from food-derived proteins to aid the discovery of new hypotensive drugs with fewer side effects. In this study, we performed high-throughput prediction of AHTPs based on multi-omics data, providing an overview of AHTPs in the lined seahorse proteins and suggesting their potential application as bioactive agents to lower blood pressure. We identified 14,695 AHTP-derived genes in the lined seahorse, and most of them were supported by transcriptomic evidence, whereas only 495 genes were further detected by proteome sequencing. Among these predicted AHTP-derived genes, the longest titin had the most hits with 104 AHTPs, some of which were clustered in exon 158, 194, and 204. Another AHTP-rich group was in the collagen family, and four AHTP-rich collagens exhibited much higher transcription in the pouch than in other examined tissues, including brain, testis, and embryos. Additionally, antihypertensive triplets, comprised of the permutations of Gly, Pro, and Lys, prevailed in all collagen sequences due to the representative XaaYaaGly repeat units. In summary, our present findings provide a solid basis for understanding the abundance of various AHTPs in the lined seahorse as well as shed light on the development of antihypertensive products and drugs using seahorses as an important resource. |
| first_indexed | 2024-04-13T21:57:58Z |
| format | Article |
| id | doaj.art-044fc41f7ebe41a18cd3f407e92ac941 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-13T21:57:58Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-044fc41f7ebe41a18cd3f407e92ac9412022-12-22T02:28:12ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-07-01910.3389/fmars.2022.863310863310High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus)Yu Huang0Xiyang Chen1Haoyue Shu2Panpan Xiao3Xueqiang Lin4Junmin Xu5Chao Bian6Chao Bian7Xinxin You8Xinxin You9Jian Yang10Qiong Shi11Qiong Shi12Qiong Shi13Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, Shenzhen, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, Shenzhen, ChinaSchool of Food and Drugs, Shenzhen Polytechnic, Shenzhen, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, Shenzhen, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, Shenzhen, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, Shenzhen, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, Shenzhen, ChinaBGI Education Center, College of Life Sciences, University of Chinese Academy of Sciences, Shenzhen, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, Shenzhen, ChinaBGI Education Center, College of Life Sciences, University of Chinese Academy of Sciences, Shenzhen, ChinaSchool of Food and Drugs, Shenzhen Polytechnic, Shenzhen, ChinaShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, Shenzhen, ChinaBGI Education Center, College of Life Sciences, University of Chinese Academy of Sciences, Shenzhen, ChinaCollege of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaRecently, the prevalence of hypertension has become a global challenge. Therefore, tremendous efforts have been made to identify and purify antihypertensive peptides (AHTPs) from food-derived proteins to aid the discovery of new hypotensive drugs with fewer side effects. In this study, we performed high-throughput prediction of AHTPs based on multi-omics data, providing an overview of AHTPs in the lined seahorse proteins and suggesting their potential application as bioactive agents to lower blood pressure. We identified 14,695 AHTP-derived genes in the lined seahorse, and most of them were supported by transcriptomic evidence, whereas only 495 genes were further detected by proteome sequencing. Among these predicted AHTP-derived genes, the longest titin had the most hits with 104 AHTPs, some of which were clustered in exon 158, 194, and 204. Another AHTP-rich group was in the collagen family, and four AHTP-rich collagens exhibited much higher transcription in the pouch than in other examined tissues, including brain, testis, and embryos. Additionally, antihypertensive triplets, comprised of the permutations of Gly, Pro, and Lys, prevailed in all collagen sequences due to the representative XaaYaaGly repeat units. In summary, our present findings provide a solid basis for understanding the abundance of various AHTPs in the lined seahorse as well as shed light on the development of antihypertensive products and drugs using seahorses as an important resource.https://www.frontiersin.org/articles/10.3389/fmars.2022.863310/fullthe lined seahorseantihypertensive peptidemulti-omics datahigh-throughput predictiontitincollagen |
| spellingShingle | Yu Huang Xiyang Chen Haoyue Shu Panpan Xiao Xueqiang Lin Junmin Xu Chao Bian Chao Bian Xinxin You Xinxin You Jian Yang Qiong Shi Qiong Shi Qiong Shi High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus) Frontiers in Marine Science the lined seahorse antihypertensive peptide multi-omics data high-throughput prediction titin collagen |
| title | High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus) |
| title_full | High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus) |
| title_fullStr | High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus) |
| title_full_unstemmed | High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus) |
| title_short | High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus) |
| title_sort | high throughput identification of antihypertensive peptides ahtps and characterization of ahtp derived genes in the lined seahorse hippocampus erectus |
| topic | the lined seahorse antihypertensive peptide multi-omics data high-throughput prediction titin collagen |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.863310/full |
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