Genome-wide identification and expression analysis of GA20ox and GA3ox genes during pod development in peanut
Background Gibberellins (GAs) play important roles in regulating peanut growth and development. GA20ox and GA3ox are key enzymes involved in GA biosynthesis. These enzymes encoded by a multigene family belong to the 2OG-Fe (II) oxygenase superfamily. To date, no genome-wide comparative analysis of p...
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PeerJ Inc.
2023-10-01
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| Online Access: | https://peerj.com/articles/16279.pdf |
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| author | Jie Sun Xiaoqian Zhang Chun Fu Naveed Ahmad Chuanzhi Zhao Lei Hou Muhammad Naeem Jiaowen Pan Xingjun Wang Shuzhen Zhao |
| author_facet | Jie Sun Xiaoqian Zhang Chun Fu Naveed Ahmad Chuanzhi Zhao Lei Hou Muhammad Naeem Jiaowen Pan Xingjun Wang Shuzhen Zhao |
| author_sort | Jie Sun |
| collection | DOAJ |
| description | Background Gibberellins (GAs) play important roles in regulating peanut growth and development. GA20ox and GA3ox are key enzymes involved in GA biosynthesis. These enzymes encoded by a multigene family belong to the 2OG-Fe (II) oxygenase superfamily. To date, no genome-wide comparative analysis of peanut AhGA20ox and AhGA3ox-encoding genes has been performed, and the roles of these genes in peanut pod development are not clear. Methods A whole-genome analysis of AhGA20ox and AhGA3ox gene families in peanut was carried out using bioinformatic tools. The expression of these genes at different stage of pod development was analyzed using qRT-PCR. Results In this study, a total of 15 AhGA20ox and five AhGA3ox genes were identified in peanut genome, which were distributed on 14 chromosomes. Phylogenetic analysis divided the GA20oxs and GA3oxs into three groups, but AhGA20oxs and AhGA3oxs in two groups. The conserved pattern of gene structure, cis-elements, and protein motifs further confirmed their evolutionary relationship in peanut. AhGA20ox and AhGA3ox genes were differential expressed at different stages of pod development. The strong expression of AhGA20ox1/AhGA20ox4, AhGA20ox12/AhGA20ox15, AhGA3ox1 and AhGA3ox4/AhGA3ox5 in S1-stage indicated that these genes could have a key role in controlling peg elongation. Furthermore, AhGA20ox and AhGA3ox also showed diverse expression patterns in different peanut tissues including leaves, main stems, flowers and inflorescences. Noticeably, AhGA20ox9/AhGA20ox11 and AhGA3o4/AhGA3ox5 were highly expressed in the main stem, whereas the AhGA3ox1 and AhGA20ox10 were strongly expressed in the inflorescence. The expression levels of AhGA20ox2/AhGA20ox3, AhGA20ox5/AhGA20ox6, AhGA20ox7/AhGA20ox8, AhGA20ox13/AhGA20ox14 and AhGA3ox2/AhGA3ox3 were high in the flowers, suggesting their involvement in flower development. These results provide a basis for deciphering the roles of AhGA20ox and AhGA3ox in peanut growth and development, especially in pod development. |
| first_indexed | 2024-03-09T05:07:41Z |
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| last_indexed | 2024-03-09T05:07:41Z |
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| spelling | doaj.art-0451bebc320a4f408578cb18328c87172023-12-03T12:52:38ZengPeerJ Inc.PeerJ2167-83592023-10-0111e1627910.7717/peerj.16279Genome-wide identification and expression analysis of GA20ox and GA3ox genes during pod development in peanutJie Sun0Xiaoqian Zhang1Chun Fu2Naveed Ahmad3Chuanzhi Zhao4Lei Hou5Muhammad Naeem6Jiaowen Pan7Xingjun Wang8Shuzhen Zhao9Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaWeifang Academy of Agricultural Sciences, Weifang, ChinaJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, PR China, Shanghai, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaBackground Gibberellins (GAs) play important roles in regulating peanut growth and development. GA20ox and GA3ox are key enzymes involved in GA biosynthesis. These enzymes encoded by a multigene family belong to the 2OG-Fe (II) oxygenase superfamily. To date, no genome-wide comparative analysis of peanut AhGA20ox and AhGA3ox-encoding genes has been performed, and the roles of these genes in peanut pod development are not clear. Methods A whole-genome analysis of AhGA20ox and AhGA3ox gene families in peanut was carried out using bioinformatic tools. The expression of these genes at different stage of pod development was analyzed using qRT-PCR. Results In this study, a total of 15 AhGA20ox and five AhGA3ox genes were identified in peanut genome, which were distributed on 14 chromosomes. Phylogenetic analysis divided the GA20oxs and GA3oxs into three groups, but AhGA20oxs and AhGA3oxs in two groups. The conserved pattern of gene structure, cis-elements, and protein motifs further confirmed their evolutionary relationship in peanut. AhGA20ox and AhGA3ox genes were differential expressed at different stages of pod development. The strong expression of AhGA20ox1/AhGA20ox4, AhGA20ox12/AhGA20ox15, AhGA3ox1 and AhGA3ox4/AhGA3ox5 in S1-stage indicated that these genes could have a key role in controlling peg elongation. Furthermore, AhGA20ox and AhGA3ox also showed diverse expression patterns in different peanut tissues including leaves, main stems, flowers and inflorescences. Noticeably, AhGA20ox9/AhGA20ox11 and AhGA3o4/AhGA3ox5 were highly expressed in the main stem, whereas the AhGA3ox1 and AhGA20ox10 were strongly expressed in the inflorescence. The expression levels of AhGA20ox2/AhGA20ox3, AhGA20ox5/AhGA20ox6, AhGA20ox7/AhGA20ox8, AhGA20ox13/AhGA20ox14 and AhGA3ox2/AhGA3ox3 were high in the flowers, suggesting their involvement in flower development. These results provide a basis for deciphering the roles of AhGA20ox and AhGA3ox in peanut growth and development, especially in pod development.https://peerj.com/articles/16279.pdfArachis hypogaea Gibberellin biosynthesisGene expression analysisPod development |
| spellingShingle | Jie Sun Xiaoqian Zhang Chun Fu Naveed Ahmad Chuanzhi Zhao Lei Hou Muhammad Naeem Jiaowen Pan Xingjun Wang Shuzhen Zhao Genome-wide identification and expression analysis of GA20ox and GA3ox genes during pod development in peanut PeerJ Arachis hypogaea Gibberellin biosynthesis Gene expression analysis Pod development |
| title | Genome-wide identification and expression analysis of GA20ox and GA3ox genes during pod development in peanut |
| title_full | Genome-wide identification and expression analysis of GA20ox and GA3ox genes during pod development in peanut |
| title_fullStr | Genome-wide identification and expression analysis of GA20ox and GA3ox genes during pod development in peanut |
| title_full_unstemmed | Genome-wide identification and expression analysis of GA20ox and GA3ox genes during pod development in peanut |
| title_short | Genome-wide identification and expression analysis of GA20ox and GA3ox genes during pod development in peanut |
| title_sort | genome wide identification and expression analysis of ga20ox and ga3ox genes during pod development in peanut |
| topic | Arachis hypogaea Gibberellin biosynthesis Gene expression analysis Pod development |
| url | https://peerj.com/articles/16279.pdf |
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