Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp.
The present work is focused on the development of an analytical platform to elucidate the metabolic pathway of PTSO from onion, an organosulfur compound well-known for its functional and technological properties and its potential application in animal and human nutrition. This analytical platform co...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/12/4/823 |
| _version_ | 1827757351174668288 |
|---|---|
| author | María García-Nicolás Marta Pastor-Belda Natalia Campillo María Jesús Rodríguez-Sojo Antonio Jesús Ruiz-Malagón Laura Hidalgo-García Paloma Abad José Manuel de la Torre Enrique Guillamón Alberto Baños Julio Gálvez Pilar Viñas Natalia Arroyo-Manzanares |
| author_facet | María García-Nicolás Marta Pastor-Belda Natalia Campillo María Jesús Rodríguez-Sojo Antonio Jesús Ruiz-Malagón Laura Hidalgo-García Paloma Abad José Manuel de la Torre Enrique Guillamón Alberto Baños Julio Gálvez Pilar Viñas Natalia Arroyo-Manzanares |
| author_sort | María García-Nicolás |
| collection | DOAJ |
| description | The present work is focused on the development of an analytical platform to elucidate the metabolic pathway of PTSO from onion, an organosulfur compound well-known for its functional and technological properties and its potential application in animal and human nutrition. This analytical platform consisted of the use of gas chromatography–mass spectrometry (GC-MS) and ultra-high performance liquid chromatography quadrupole with time-of-flight MS (UHPLC-Q-TOF-MS) in order to monitor volatile and non-volatile compounds derived from the PTSO. For the extraction of the compounds of interest, two different sample treatments were developed: liquid–liquid extraction (LLE) and salting-out assisted liquid–liquid extraction (SALLE) for GC–MS and UHPLC-Q-TOF-MS analysis, respectively. Once the analytical platform was optimised and validated, an in vivo study was planned to elucidate PTSO metabolisation, revealing the presence of dipropyl disulfide (DPDS) in liver samples with concentrations between 0.11 and 0.61 µg g<sup>−1</sup>. The DPDS maximum concentration in the liver was observed at 0.5 h after the intake. DPDS was also present in all plasma samples with concentrations between 2.1 and 2.4 µg mL<sup>−1</sup>. In regard to PTSO, it was only found in plasma at times above 5 h (0.18 µg mL<sup>−1</sup>). Both PTSO and DPDS were excreted via urine 24 h after ingestion. |
| first_indexed | 2024-03-11T08:49:28Z |
| format | Article |
| id | doaj.art-04550605ca6048859806739690f0ab1b |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T08:49:28Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-04550605ca6048859806739690f0ab1b2023-11-16T20:31:02ZengMDPI AGFoods2304-81582023-02-0112482310.3390/foods12040823Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp.María García-Nicolás0Marta Pastor-Belda1Natalia Campillo2María Jesús Rodríguez-Sojo3Antonio Jesús Ruiz-Malagón4Laura Hidalgo-García5Paloma Abad6José Manuel de la Torre7Enrique Guillamón8Alberto Baños9Julio Gálvez10Pilar Viñas11Natalia Arroyo-Manzanares12Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, SpainDepartment of Pharmacology, Instituto de Investigación Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, SpainDepartment of Pharmacology, Instituto de Investigación Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, SpainDepartment of Pharmacology, Instituto de Investigación Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, SpainDepartment of Inorganic and Organic Chemistry, Campus of Lagunillas, Universidad de Jaén, E-23071 Jaén, SpainDepartment of Microbiology, University of Granada, Fuente Nueva s/n, E-19071 Granada, SpainDepartment of Microbiology, University of Granada, Fuente Nueva s/n, E-19071 Granada, SpainDepartment of Pharmacology, Instituto de Investigación Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, SpainThe present work is focused on the development of an analytical platform to elucidate the metabolic pathway of PTSO from onion, an organosulfur compound well-known for its functional and technological properties and its potential application in animal and human nutrition. This analytical platform consisted of the use of gas chromatography–mass spectrometry (GC-MS) and ultra-high performance liquid chromatography quadrupole with time-of-flight MS (UHPLC-Q-TOF-MS) in order to monitor volatile and non-volatile compounds derived from the PTSO. For the extraction of the compounds of interest, two different sample treatments were developed: liquid–liquid extraction (LLE) and salting-out assisted liquid–liquid extraction (SALLE) for GC–MS and UHPLC-Q-TOF-MS analysis, respectively. Once the analytical platform was optimised and validated, an in vivo study was planned to elucidate PTSO metabolisation, revealing the presence of dipropyl disulfide (DPDS) in liver samples with concentrations between 0.11 and 0.61 µg g<sup>−1</sup>. The DPDS maximum concentration in the liver was observed at 0.5 h after the intake. DPDS was also present in all plasma samples with concentrations between 2.1 and 2.4 µg mL<sup>−1</sup>. In regard to PTSO, it was only found in plasma at times above 5 h (0.18 µg mL<sup>−1</sup>). Both PTSO and DPDS were excreted via urine 24 h after ingestion.https://www.mdpi.com/2304-8158/12/4/823propyl propane thiosulfonateratsmetabolisationin vivo studyanalytical platform |
| spellingShingle | María García-Nicolás Marta Pastor-Belda Natalia Campillo María Jesús Rodríguez-Sojo Antonio Jesús Ruiz-Malagón Laura Hidalgo-García Paloma Abad José Manuel de la Torre Enrique Guillamón Alberto Baños Julio Gálvez Pilar Viñas Natalia Arroyo-Manzanares Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp. Foods propyl propane thiosulfonate rats metabolisation in vivo study analytical platform |
| title | Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp. |
| title_full | Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp. |
| title_fullStr | Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp. |
| title_full_unstemmed | Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp. |
| title_short | Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp. |
| title_sort | analytical platform for the study of metabolic pathway of propyl propane thiosulfonate ptso from i allium i spp |
| topic | propyl propane thiosulfonate rats metabolisation in vivo study analytical platform |
| url | https://www.mdpi.com/2304-8158/12/4/823 |
| work_keys_str_mv | AT mariagarcianicolas analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT martapastorbelda analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT nataliacampillo analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT mariajesusrodriguezsojo analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT antoniojesusruizmalagon analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT laurahidalgogarcia analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT palomaabad analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT josemanueldelatorre analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT enriqueguillamon analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT albertobanos analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT juliogalvez analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT pilarvinas analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp AT nataliaarroyomanzanares analyticalplatformforthestudyofmetabolicpathwayofpropylpropanethiosulfonateptsofromialliumispp |