miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16
Abstract Background Increasing reports demonstrated that dysregulated expression of microRNAs (miRNAs) leads to the progression of various tumors. Previous studies revealed that miR-328-3p exhibited dysregulated expression in various types of tumors. However, its function and underlying mechanism in...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-04-01
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| Series: | Cancer Cell International |
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| Online Access: | http://link.springer.com/article/10.1186/s12935-019-0829-7 |
| _version_ | 1811299523025174528 |
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| author | Jianhui Shi Gang An Ying Guan Tianli Wei Zhibin Peng Min Liang Yansong Wang |
| author_facet | Jianhui Shi Gang An Ying Guan Tianli Wei Zhibin Peng Min Liang Yansong Wang |
| author_sort | Jianhui Shi |
| collection | DOAJ |
| description | Abstract Background Increasing reports demonstrated that dysregulated expression of microRNAs (miRNAs) leads to the progression of various tumors. Previous studies revealed that miR-328-3p exhibited dysregulated expression in various types of tumors. However, its function and underlying mechanism in osteosarcoma (OS) are still unexplored. Methods The expression of miR-328-3p in the tissues and OS cell lines was detected by qRT-PCR analysis. The effects of miR-328-3p in the proliferation were analyzed by MTT assay. The proliferation and apoptosis of OS cells were examined by colony formation assay and TUNEL staining respectively. The migration and tumor formation ability of OS cells were measured by wound healing assay and xenograft in vivo mice assay. Furthermore, the regulatory roles of miR-328-3p/MMP16 were determined by western blot and luciferase reporter assay. Results The expression of miR-328-3p was significantly decreased in OS tissues and cell lines. Furthermore, overexpression of miR-328-3p inhibited the cell proliferation and migration, but promoted the apoptosis of OS cells in vitro. Moreover, the analysis in vivo showed that miR-328-3p effectively suppressed the formation of tumors. According to the results of western blot analysis and luciferase reporter assay, we identified matrix metalloproteinase-16 (MMP-16) acted as a direct target of miR-328-3p. Moreover, the expression level of MMP-16, which participates in the occurrence and development of many cancers, was negatively correlated with the miR-328-3p expression in OS cells. Conclusion miR-328-3p inhibited the proliferation, migration but accelerated the apoptosis of OS by directly inhibiting MMP-16. And miR-328-3p/MMP-16 axis may be one of the mechanisms of OS development and a novel potential method for the treatment of OS in clinic. |
| first_indexed | 2024-04-13T06:37:05Z |
| format | Article |
| id | doaj.art-04550baf9b4d42ecb36d37dd4a0ab60f |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-13T06:37:05Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-04550baf9b4d42ecb36d37dd4a0ab60f2022-12-22T02:57:52ZengBMCCancer Cell International1475-28672019-04-0119111210.1186/s12935-019-0829-7miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16Jianhui Shi0Gang An1Ying Guan2Tianli Wei3Zhibin Peng4Min Liang5Yansong Wang6Department of Spine Surgery, The First Affiliated Hospital of Harbin Medical UniversityDepartment of Spine Surgery, The First Affiliated Hospital of Harbin Medical UniversityDepartment of Spine Surgery, The First Affiliated Hospital of Harbin Medical UniversityDepartment of Spine Surgery, The First Affiliated Hospital of Harbin Medical UniversityDepartment of Spine Surgery, The First Affiliated Hospital of Harbin Medical UniversityDepartment of Spine Surgery, The First Affiliated Hospital of Harbin Medical UniversityDepartment of Spine Surgery, The First Affiliated Hospital of Harbin Medical UniversityAbstract Background Increasing reports demonstrated that dysregulated expression of microRNAs (miRNAs) leads to the progression of various tumors. Previous studies revealed that miR-328-3p exhibited dysregulated expression in various types of tumors. However, its function and underlying mechanism in osteosarcoma (OS) are still unexplored. Methods The expression of miR-328-3p in the tissues and OS cell lines was detected by qRT-PCR analysis. The effects of miR-328-3p in the proliferation were analyzed by MTT assay. The proliferation and apoptosis of OS cells were examined by colony formation assay and TUNEL staining respectively. The migration and tumor formation ability of OS cells were measured by wound healing assay and xenograft in vivo mice assay. Furthermore, the regulatory roles of miR-328-3p/MMP16 were determined by western blot and luciferase reporter assay. Results The expression of miR-328-3p was significantly decreased in OS tissues and cell lines. Furthermore, overexpression of miR-328-3p inhibited the cell proliferation and migration, but promoted the apoptosis of OS cells in vitro. Moreover, the analysis in vivo showed that miR-328-3p effectively suppressed the formation of tumors. According to the results of western blot analysis and luciferase reporter assay, we identified matrix metalloproteinase-16 (MMP-16) acted as a direct target of miR-328-3p. Moreover, the expression level of MMP-16, which participates in the occurrence and development of many cancers, was negatively correlated with the miR-328-3p expression in OS cells. Conclusion miR-328-3p inhibited the proliferation, migration but accelerated the apoptosis of OS by directly inhibiting MMP-16. And miR-328-3p/MMP-16 axis may be one of the mechanisms of OS development and a novel potential method for the treatment of OS in clinic.http://link.springer.com/article/10.1186/s12935-019-0829-7MiRNAsOsteosarcomaProliferationMigrationMMP-16 |
| spellingShingle | Jianhui Shi Gang An Ying Guan Tianli Wei Zhibin Peng Min Liang Yansong Wang miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16 Cancer Cell International MiRNAs Osteosarcoma Proliferation Migration MMP-16 |
| title | miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16 |
| title_full | miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16 |
| title_fullStr | miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16 |
| title_full_unstemmed | miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16 |
| title_short | miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16 |
| title_sort | mir 328 3p mediates the anti tumor effect in osteosarcoma via directly targeting mmp 16 |
| topic | MiRNAs Osteosarcoma Proliferation Migration MMP-16 |
| url | http://link.springer.com/article/10.1186/s12935-019-0829-7 |
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