ASSOCIATION OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) WITH PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9 (PCSK9) IN FAMILIAL HYPERCHOLESTEROLEMIA
Objective was to study the levels and the relationship between lipoprotein-associated phospholipase A2 (Lp-PLA2) and proprotein convertase subtilisin/kexin type 9 (PCSK9), their relationship with other lipid parameters in patients with familial hypercholesterolemia (FH) in Russia. Material and me...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Siberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and Genetics
2019-03-01
|
| Series: | Атеросклероз |
| Subjects: | |
| Online Access: | https://ateroskleroz.elpub.ru/jour/article/view/151/131 |
| _version_ | 1797962986765156352 |
|---|---|
| author | K. S. Benimetskaya V. S. Shramko E. M. Stakhneva K. V. Makarenkova L. V. Shcherbakova Yu. I. Ragino M. I. Voevoda |
| author_facet | K. S. Benimetskaya V. S. Shramko E. M. Stakhneva K. V. Makarenkova L. V. Shcherbakova Yu. I. Ragino M. I. Voevoda |
| author_sort | K. S. Benimetskaya |
| collection | DOAJ |
| description | Objective was to study the levels and the relationship between lipoprotein-associated phospholipase A2 (Lp-PLA2) and proprotein convertase subtilisin/kexin type 9 (PCSK9), their relationship with other lipid parameters in patients with familial hypercholesterolemia (FH) in Russia.
Material and methods. The study involved 47 patients (11 males) observed in the Scientific-clinical center of lipidology of Research Institute of Internal and Preventive Medicine - Branch of the Institute of Cytology and Genetics of SB RAS with diagnosis of «possible» or «definite» FH according to the criteria of The Simon Broome Register Group and «probable» or «definite» FH according to the Dutch Lipid Clinic Network Criteria. Patient age was 49.13 ± 12.67 years (mean ± SD); 50.00 [40.00; 59.00] years (median [lower quartile; upper quartile]). Lp-PLA2 and PCSK9 content was determined by enzyme-linked immunosorbent assay using a test systems «Human Proprotein Convertase 9/PCSK9 Immunoassay» («R&D Systems», USA) and «ELISA Kit for Phospholipase A2 Group VII (LpPLA2)» («Cloud-Clone Corp.», USA).
Results. Lp-PLA2 level was 73,69 [64,99; 106,53] ng/ ml, PCSK9 – 352,16 [272,94; 416,79] ng/ml. There was a trend to moderate positive correlation between Lp-PLA2 and PCSK9 content (r = 0.614; p = 0.059), the statistical significance value was borderline due to the small number surveyed. Males showed a strong positive correlation of LP-FLA2 concentration with age (r = 0.746; p = 0.008), partial – with triglyceride (TG) content (r = 0.793; p = 0.019). A strong positive correlation of PCSK9 level with apolipoprotein B / apolipoprotein A ratio (r = 0.702; p = 0.007) and a weak positive correlation with TG concentration (r = 0.330; p = 0.033) were shown in the general group. Moderate positive correlation of PCSK9 content with age (r = 0.660; p = 0.038), a strong negative correlation with total cholesterol (r = –0.815; p = 0.004), low-density lipoprotein cholesterol (r = –0.828; p = 0.006) and non-high-density lipoprotein cholesterol (r = –0.851, p = 0.002) concentration were shown. Women with TG level <1.7 mmol/l had lower PCSK9 level then women with TG level ≥1.7 mmol/l (328.45 [231.02; 387.82 and 397.12 [348.45; 531.62] ng/ml, respectively (p = 0.013)).
Conclusion. The correlation of PCSK9, the perspective marker of cardiovascular diseases, with the avowed marker Lp-PLA2 confirms the importance of PCSK9 in lipid metabolism and cardiovascular homeostasis and is the basis for its further research as a biological marker of cardiovascular diseases. |
| first_indexed | 2024-04-11T01:22:19Z |
| format | Article |
| id | doaj.art-04599c1398274c2a8ab459d4111f9c1a |
| institution | Directory Open Access Journal |
| issn | 2078-256X |
| language | Russian |
| last_indexed | 2024-04-11T01:22:19Z |
| publishDate | 2019-03-01 |
| publisher | Siberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and Genetics |
| record_format | Article |
| series | Атеросклероз |
| spelling | doaj.art-04599c1398274c2a8ab459d4111f9c1a2023-01-03T11:12:18ZrusSiberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and GeneticsАтеросклероз2078-256X2019-03-011511523https://doi.org/10.15372/ATER20190102ASSOCIATION OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) WITH PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9 (PCSK9) IN FAMILIAL HYPERCHOLESTEROLEMIAK. S. Benimetskaya0https://orcid.org/0000-0002-0043-1113V. S. Shramko1https://orcid.org/0000-0002-0436-2549E. M. Stakhneva2K. V. Makarenkova3L. V. Shcherbakova4Yu. I. Ragino5https://orcid.org/0000-0002-4936-8362M. I. Voevoda6https://orcid.org/0000-0001-9425-413XInstitute of Internal and Preventive Medicine – Branch of Federal Research Institute of Cytology and Genetics of SB RAS ; Novosibirsk National Research State University Russian FederationInstitute of Internal and Preventive Medicine – Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian FederationInstitute of Internal and Preventive Medicine – Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian FederationInstitute of Internal and Preventive Medicine – Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian FederationInstitute of Internal and Preventive Medicine – Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian FederationInstitute of Internal and Preventive Medicine – Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian FederationInstitute of Internal and Preventive Medicine – Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian FederationObjective was to study the levels and the relationship between lipoprotein-associated phospholipase A2 (Lp-PLA2) and proprotein convertase subtilisin/kexin type 9 (PCSK9), their relationship with other lipid parameters in patients with familial hypercholesterolemia (FH) in Russia. Material and methods. The study involved 47 patients (11 males) observed in the Scientific-clinical center of lipidology of Research Institute of Internal and Preventive Medicine - Branch of the Institute of Cytology and Genetics of SB RAS with diagnosis of «possible» or «definite» FH according to the criteria of The Simon Broome Register Group and «probable» or «definite» FH according to the Dutch Lipid Clinic Network Criteria. Patient age was 49.13 ± 12.67 years (mean ± SD); 50.00 [40.00; 59.00] years (median [lower quartile; upper quartile]). Lp-PLA2 and PCSK9 content was determined by enzyme-linked immunosorbent assay using a test systems «Human Proprotein Convertase 9/PCSK9 Immunoassay» («R&D Systems», USA) and «ELISA Kit for Phospholipase A2 Group VII (LpPLA2)» («Cloud-Clone Corp.», USA). Results. Lp-PLA2 level was 73,69 [64,99; 106,53] ng/ ml, PCSK9 – 352,16 [272,94; 416,79] ng/ml. There was a trend to moderate positive correlation between Lp-PLA2 and PCSK9 content (r = 0.614; p = 0.059), the statistical significance value was borderline due to the small number surveyed. Males showed a strong positive correlation of LP-FLA2 concentration with age (r = 0.746; p = 0.008), partial – with triglyceride (TG) content (r = 0.793; p = 0.019). A strong positive correlation of PCSK9 level with apolipoprotein B / apolipoprotein A ratio (r = 0.702; p = 0.007) and a weak positive correlation with TG concentration (r = 0.330; p = 0.033) were shown in the general group. Moderate positive correlation of PCSK9 content with age (r = 0.660; p = 0.038), a strong negative correlation with total cholesterol (r = –0.815; p = 0.004), low-density lipoprotein cholesterol (r = –0.828; p = 0.006) and non-high-density lipoprotein cholesterol (r = –0.851, p = 0.002) concentration were shown. Women with TG level <1.7 mmol/l had lower PCSK9 level then women with TG level ≥1.7 mmol/l (328.45 [231.02; 387.82 and 397.12 [348.45; 531.62] ng/ml, respectively (p = 0.013)). Conclusion. The correlation of PCSK9, the perspective marker of cardiovascular diseases, with the avowed marker Lp-PLA2 confirms the importance of PCSK9 in lipid metabolism and cardiovascular homeostasis and is the basis for its further research as a biological marker of cardiovascular diseases.https://ateroskleroz.elpub.ru/jour/article/view/151/131lipoprotein-associated phospholipase a2lp-pla2proprotein convertase subtilisin/ kexin type 9pcsk9familial hypercholesterolemia |
| spellingShingle | K. S. Benimetskaya V. S. Shramko E. M. Stakhneva K. V. Makarenkova L. V. Shcherbakova Yu. I. Ragino M. I. Voevoda ASSOCIATION OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) WITH PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9 (PCSK9) IN FAMILIAL HYPERCHOLESTEROLEMIA Атеросклероз lipoprotein-associated phospholipase a2 lp-pla2 proprotein convertase subtilisin/ kexin type 9 pcsk9 familial hypercholesterolemia |
| title | ASSOCIATION OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) WITH PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9 (PCSK9) IN FAMILIAL HYPERCHOLESTEROLEMIA |
| title_full | ASSOCIATION OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) WITH PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9 (PCSK9) IN FAMILIAL HYPERCHOLESTEROLEMIA |
| title_fullStr | ASSOCIATION OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) WITH PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9 (PCSK9) IN FAMILIAL HYPERCHOLESTEROLEMIA |
| title_full_unstemmed | ASSOCIATION OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) WITH PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9 (PCSK9) IN FAMILIAL HYPERCHOLESTEROLEMIA |
| title_short | ASSOCIATION OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) WITH PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9 (PCSK9) IN FAMILIAL HYPERCHOLESTEROLEMIA |
| title_sort | association of lipoprotein associated phospholipase a2 lp pla2 with proprotein convertase subtilisin kexin type 9 pcsk9 in familial hypercholesterolemia |
| topic | lipoprotein-associated phospholipase a2 lp-pla2 proprotein convertase subtilisin/ kexin type 9 pcsk9 familial hypercholesterolemia |
| url | https://ateroskleroz.elpub.ru/jour/article/view/151/131 |
| work_keys_str_mv | AT ksbenimetskaya associationoflipoproteinassociatedphospholipasea2lppla2withproproteinconvertasesubtilisinkexintype9pcsk9infamilialhypercholesterolemia AT vsshramko associationoflipoproteinassociatedphospholipasea2lppla2withproproteinconvertasesubtilisinkexintype9pcsk9infamilialhypercholesterolemia AT emstakhneva associationoflipoproteinassociatedphospholipasea2lppla2withproproteinconvertasesubtilisinkexintype9pcsk9infamilialhypercholesterolemia AT kvmakarenkova associationoflipoproteinassociatedphospholipasea2lppla2withproproteinconvertasesubtilisinkexintype9pcsk9infamilialhypercholesterolemia AT lvshcherbakova associationoflipoproteinassociatedphospholipasea2lppla2withproproteinconvertasesubtilisinkexintype9pcsk9infamilialhypercholesterolemia AT yuiragino associationoflipoproteinassociatedphospholipasea2lppla2withproproteinconvertasesubtilisinkexintype9pcsk9infamilialhypercholesterolemia AT mivoevoda associationoflipoproteinassociatedphospholipasea2lppla2withproproteinconvertasesubtilisinkexintype9pcsk9infamilialhypercholesterolemia |