Investigation of urediospore morphology, histopathology and epidemiological components on wheat plants infected with UV‐B‐induced mutant strains of Puccinia striiformis f. sp. tritici
Abstract Planting resistant cultivars is the most economical and effective measure to control wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), but the cultivars often lose their resistance due to the emergence of new physiological races. The UV‐B‐irradiated urediospores of the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.870 |
| _version_ | 1819069926584877056 |
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| author | Yaqiong Zhao Pei Cheng Tingting Li Jinxing Ma Yuzhu Zhang Haiguang Wang |
| author_facet | Yaqiong Zhao Pei Cheng Tingting Li Jinxing Ma Yuzhu Zhang Haiguang Wang |
| author_sort | Yaqiong Zhao |
| collection | DOAJ |
| description | Abstract Planting resistant cultivars is the most economical and effective measure to control wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), but the cultivars often lose their resistance due to the emergence of new physiological races. The UV‐B‐irradiated urediospores of the Pst physiological race CYR32 in China were inoculated on wheat cultivar Guinong 22 for screening virulence‐mutant strains. CYR32 and mutant strains (CYR32‐5 and CYR32‐61) before and after UV‐B radiation were used to conduct urediospore morphological and histopathological observations and an investigation of epidemiological components. The results showed that UV‐B radiation affected the urediospore morphology of each strain. UV‐B radiation inhibited urediospore invasion and hyphal elongation, which mainly manifested as decreases in germination rate, quantities of hyphal branches, haustorial mother cells and haustoria and hyphal length. After wheat cultivar Mingxian 169 was inoculated with the UV‐B‐irradiated urediospores, the incubation period was prolonged, and the infection efficiency, lesion expansion rate, total sporulation quantity and area under the disease progress curve were reduced. The results demonstrated that CYR32‐5 and CYR32‐61 may have more tolerance to UV‐B radiation than CYR32. The results are significant for understanding mechanisms of Pst virulence variations and implementing sustainable management of wheat stripe rust. |
| first_indexed | 2024-12-21T16:57:48Z |
| format | Article |
| id | doaj.art-045cc91ef2234c82821e10ac3863acbc |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-21T16:57:48Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-045cc91ef2234c82821e10ac3863acbc2022-12-21T18:56:43ZengWileyMicrobiologyOpen2045-88272019-10-01810n/an/a10.1002/mbo3.870Investigation of urediospore morphology, histopathology and epidemiological components on wheat plants infected with UV‐B‐induced mutant strains of Puccinia striiformis f. sp. triticiYaqiong Zhao0Pei Cheng1Tingting Li2Jinxing Ma3Yuzhu Zhang4Haiguang Wang5Department of Plant Pathology, College of Plant Protection China Agricultural University Beijing ChinaDepartment of Plant Pathology, College of Plant Protection China Agricultural University Beijing ChinaDepartment of Plant Pathology, College of Plant Protection China Agricultural University Beijing ChinaDepartment of Plant Pathology, College of Plant Protection China Agricultural University Beijing ChinaDepartment of Plant Pathology, College of Plant Protection China Agricultural University Beijing ChinaDepartment of Plant Pathology, College of Plant Protection China Agricultural University Beijing ChinaAbstract Planting resistant cultivars is the most economical and effective measure to control wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), but the cultivars often lose their resistance due to the emergence of new physiological races. The UV‐B‐irradiated urediospores of the Pst physiological race CYR32 in China were inoculated on wheat cultivar Guinong 22 for screening virulence‐mutant strains. CYR32 and mutant strains (CYR32‐5 and CYR32‐61) before and after UV‐B radiation were used to conduct urediospore morphological and histopathological observations and an investigation of epidemiological components. The results showed that UV‐B radiation affected the urediospore morphology of each strain. UV‐B radiation inhibited urediospore invasion and hyphal elongation, which mainly manifested as decreases in germination rate, quantities of hyphal branches, haustorial mother cells and haustoria and hyphal length. After wheat cultivar Mingxian 169 was inoculated with the UV‐B‐irradiated urediospores, the incubation period was prolonged, and the infection efficiency, lesion expansion rate, total sporulation quantity and area under the disease progress curve were reduced. The results demonstrated that CYR32‐5 and CYR32‐61 may have more tolerance to UV‐B radiation than CYR32. The results are significant for understanding mechanisms of Pst virulence variations and implementing sustainable management of wheat stripe rust.https://doi.org/10.1002/mbo3.870epidemiological componenthistopathologypathogen morphologyPuccinia striiformis f. sp. triticiUV‐B radiationvirulence variation |
| spellingShingle | Yaqiong Zhao Pei Cheng Tingting Li Jinxing Ma Yuzhu Zhang Haiguang Wang Investigation of urediospore morphology, histopathology and epidemiological components on wheat plants infected with UV‐B‐induced mutant strains of Puccinia striiformis f. sp. tritici MicrobiologyOpen epidemiological component histopathology pathogen morphology Puccinia striiformis f. sp. tritici UV‐B radiation virulence variation |
| title | Investigation of urediospore morphology, histopathology and epidemiological components on wheat plants infected with UV‐B‐induced mutant strains of Puccinia striiformis f. sp. tritici |
| title_full | Investigation of urediospore morphology, histopathology and epidemiological components on wheat plants infected with UV‐B‐induced mutant strains of Puccinia striiformis f. sp. tritici |
| title_fullStr | Investigation of urediospore morphology, histopathology and epidemiological components on wheat plants infected with UV‐B‐induced mutant strains of Puccinia striiformis f. sp. tritici |
| title_full_unstemmed | Investigation of urediospore morphology, histopathology and epidemiological components on wheat plants infected with UV‐B‐induced mutant strains of Puccinia striiformis f. sp. tritici |
| title_short | Investigation of urediospore morphology, histopathology and epidemiological components on wheat plants infected with UV‐B‐induced mutant strains of Puccinia striiformis f. sp. tritici |
| title_sort | investigation of urediospore morphology histopathology and epidemiological components on wheat plants infected with uv b induced mutant strains of puccinia striiformis f sp tritici |
| topic | epidemiological component histopathology pathogen morphology Puccinia striiformis f. sp. tritici UV‐B radiation virulence variation |
| url | https://doi.org/10.1002/mbo3.870 |
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