Melatonin Administration Accelerates Puberty Onset in Mice by Promoting FSH Synthesis
Although melatonin has been extensively studied in animal reproduction, the mechanism of melatonin in puberty remains elusive. This study was designed to explore the effect of intraperitoneal administration of melatonin on puberty onset in female mice. The injection of melatonin into postnatal days...
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| author | Chan Yang Zaohong Ran Guoshi Liu Rong Hou Changjiu He Qinghua Liu Yingjun Chen Yuliang Liu Xiaodong Wang Chuqi Ling Fang Fang Xiang Li |
| author_facet | Chan Yang Zaohong Ran Guoshi Liu Rong Hou Changjiu He Qinghua Liu Yingjun Chen Yuliang Liu Xiaodong Wang Chuqi Ling Fang Fang Xiang Li |
| author_sort | Chan Yang |
| collection | DOAJ |
| description | Although melatonin has been extensively studied in animal reproduction, the mechanism of melatonin in puberty remains elusive. This study was designed to explore the effect of intraperitoneal administration of melatonin on puberty onset in female mice. The injection of melatonin into postnatal days 10 mice at a dose of 15 mg/kg accelerated the puberty onset in mice. Mechanistically, there was no difference in physical growth and serum Leptin levels after melatonin administration. Meanwhile, the serum levels of reproductive hormones involved in hypothalamic-pituitary-ovarian axis, such as FSH and estrogen level in serum were increased. The mRNA levels of <i>GnRH</i> and <i>GnRHr</i> were not affected by melatonin, while the expressions of <i>FSHβ</i> in pituitary and <i>Cyp19a1</i> in ovary were significantly up-regulated. In addition, melatonin still promoted FSH synthesis after ovariectomy. Furthermore, the enhanced activity of ERK1/2 signaling verified that the expression of <i>FSHβ</i> increased in pituitary. We confirmed that melatonin promoted the FSH synthesis in pituitary, thereby increased serum estrogen levels and ultimately accelerated puberty onset. However, these effects of melatonin may be pharmacological due to the high dose. This study would help us to understand the functions of melatonin in pubertal regulation comprehensively. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:25:44Z |
| publishDate | 2021-03-01 |
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| series | Molecules |
| spelling | doaj.art-045cee9ad36945ae962644541a173e0d2023-11-21T09:40:01ZengMDPI AGMolecules1420-30492021-03-01265147410.3390/molecules26051474Melatonin Administration Accelerates Puberty Onset in Mice by Promoting FSH SynthesisChan Yang0Zaohong Ran1Guoshi Liu2Rong Hou3Changjiu He4Qinghua Liu5Yingjun Chen6Yuliang Liu7Xiaodong Wang8Chuqi Ling9Fang Fang10Xiang Li11Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100083, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu 610081, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu 610081, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, National Center for International Research, Department of Hubei Province Engineering Research Center in Buffalo Breeding and Products, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaAlthough melatonin has been extensively studied in animal reproduction, the mechanism of melatonin in puberty remains elusive. This study was designed to explore the effect of intraperitoneal administration of melatonin on puberty onset in female mice. The injection of melatonin into postnatal days 10 mice at a dose of 15 mg/kg accelerated the puberty onset in mice. Mechanistically, there was no difference in physical growth and serum Leptin levels after melatonin administration. Meanwhile, the serum levels of reproductive hormones involved in hypothalamic-pituitary-ovarian axis, such as FSH and estrogen level in serum were increased. The mRNA levels of <i>GnRH</i> and <i>GnRHr</i> were not affected by melatonin, while the expressions of <i>FSHβ</i> in pituitary and <i>Cyp19a1</i> in ovary were significantly up-regulated. In addition, melatonin still promoted FSH synthesis after ovariectomy. Furthermore, the enhanced activity of ERK1/2 signaling verified that the expression of <i>FSHβ</i> increased in pituitary. We confirmed that melatonin promoted the FSH synthesis in pituitary, thereby increased serum estrogen levels and ultimately accelerated puberty onset. However, these effects of melatonin may be pharmacological due to the high dose. This study would help us to understand the functions of melatonin in pubertal regulation comprehensively.https://www.mdpi.com/1420-3049/26/5/1474melatoninpuberty onsetreproductive hormonespituitaryovary |
| spellingShingle | Chan Yang Zaohong Ran Guoshi Liu Rong Hou Changjiu He Qinghua Liu Yingjun Chen Yuliang Liu Xiaodong Wang Chuqi Ling Fang Fang Xiang Li Melatonin Administration Accelerates Puberty Onset in Mice by Promoting FSH Synthesis Molecules melatonin puberty onset reproductive hormones pituitary ovary |
| title | Melatonin Administration Accelerates Puberty Onset in Mice by Promoting FSH Synthesis |
| title_full | Melatonin Administration Accelerates Puberty Onset in Mice by Promoting FSH Synthesis |
| title_fullStr | Melatonin Administration Accelerates Puberty Onset in Mice by Promoting FSH Synthesis |
| title_full_unstemmed | Melatonin Administration Accelerates Puberty Onset in Mice by Promoting FSH Synthesis |
| title_short | Melatonin Administration Accelerates Puberty Onset in Mice by Promoting FSH Synthesis |
| title_sort | melatonin administration accelerates puberty onset in mice by promoting fsh synthesis |
| topic | melatonin puberty onset reproductive hormones pituitary ovary |
| url | https://www.mdpi.com/1420-3049/26/5/1474 |
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