Anti-colon cancer activities of green-synthesized Moringa oleifera–AgNPs against human colon cancer cells
The anticancer activity of silver nanoparticles (AgNPs) is well known to be synthesized using green-synthesized methods, although its mechanism of action is not understood fully. Moringa oleifera leaves were used as reducing and stabilizing agents to synthesize AgNPs. Green-synthesized AgNPs were ch...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2022-05-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2022-0052 |
| _version_ | 1811203698526781440 |
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| author | Althomali Arwa Daghestani Maha H. Basil Almukaynizi Fatimah Al-Zahrani Sabah Ahmed Awad Manal A. Merghani Nada M. Bukhari Wadha I. Ibrahim Eiman M. Alzahrani Sherifah M. Altowair Nouf AL-Ghamdi Afaf S. AlQahtani Asma M. Ramadan Rasha Bhat Ramesa Shafi |
| author_facet | Althomali Arwa Daghestani Maha H. Basil Almukaynizi Fatimah Al-Zahrani Sabah Ahmed Awad Manal A. Merghani Nada M. Bukhari Wadha I. Ibrahim Eiman M. Alzahrani Sherifah M. Altowair Nouf AL-Ghamdi Afaf S. AlQahtani Asma M. Ramadan Rasha Bhat Ramesa Shafi |
| author_sort | Althomali Arwa |
| collection | DOAJ |
| description | The anticancer activity of silver nanoparticles (AgNPs) is well known to be synthesized using green-synthesized methods, although its mechanism of action is not understood fully. Moringa oleifera leaves were used as reducing and stabilizing agents to synthesize AgNPs. Green-synthesized AgNPs were characterized using ultraviolet-visible spectroscopy, dynamic light scattering, transmission electronic microscopy, scanning electronic microscopy, Fourier transform infrared, and energy-dispersive X-ray spectroscopy analyses. The synthesized nanoparticles were then characterized by their anticancer properties by performing a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The real-time polymerase chain reaction was used to check the expression levels of the four genes (β-catenin, adenomatous polyposis coli (APC), and lipoprotein receptor-related proteins 5 and 6 (LRP5/6)). The synthesized nanoparticles were 25 nm on average and spherical in shape and aggregated form. Noteworthy cytotoxicity is how green-synthesized M. oleifera–AgNPs were observed in comparison with the M. oleifera leaf extract against a cancerous cell line. The M. oleifera–AgNPs decreased the expression of CTNNB1 and LRP6 genes, while the LRP5 gene expression increased in both cell lines. With treatment, the APC gene expression decreased in SW480 but increased in HTC116. Our results imply that AgNPs synthesized by M. oleifera extract could be an ideal strategy to combat colon cancer. |
| first_indexed | 2024-04-12T02:59:04Z |
| format | Article |
| id | doaj.art-045e294dec2e4372aa36781be03b1d03 |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-04-12T02:59:04Z |
| publishDate | 2022-05-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-045e294dec2e4372aa36781be03b1d032022-12-22T03:50:42ZengDe GruyterGreen Processing and Synthesis2191-95502022-05-0111154555410.1515/gps-2022-0052Anti-colon cancer activities of green-synthesized Moringa oleifera–AgNPs against human colon cancer cellsAlthomali Arwa0Daghestani Maha H.1Basil Almukaynizi Fatimah2Al-Zahrani Sabah Ahmed3Awad Manal A.4Merghani Nada M.5Bukhari Wadha I.6Ibrahim Eiman M.7Alzahrani Sherifah M.8Altowair Nouf9AL-Ghamdi Afaf S.10AlQahtani Asma M.11Ramadan Rasha12Bhat Ramesa Shafi13Prince Naif Bin Abdul Aziz Health Research Center, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, Centre for Scientific and Medical Female Colleges, King Saud University, Riyadh, Saudi ArabiaPrince Naif Bin Abdul Aziz Health Research Center, King Saud University, Riyadh, Saudi ArabiaBiochemistry Department, College of Science, King Saud University, PO Box 22452, Riyadh 11495, Saudi ArabiaKing Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi ArabiaCentral Research Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi ArabiaCentral Research Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi ArabiaCentral Research Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi ArabiaCentral Research Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi ArabiaCentral Research Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi ArabiaCentral Research Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi ArabiaCentral Research Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi ArabiaCentral Research Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi ArabiaBiochemistry Department, College of Science, King Saud University, PO Box 22452, Riyadh 11495, Saudi ArabiaThe anticancer activity of silver nanoparticles (AgNPs) is well known to be synthesized using green-synthesized methods, although its mechanism of action is not understood fully. Moringa oleifera leaves were used as reducing and stabilizing agents to synthesize AgNPs. Green-synthesized AgNPs were characterized using ultraviolet-visible spectroscopy, dynamic light scattering, transmission electronic microscopy, scanning electronic microscopy, Fourier transform infrared, and energy-dispersive X-ray spectroscopy analyses. The synthesized nanoparticles were then characterized by their anticancer properties by performing a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The real-time polymerase chain reaction was used to check the expression levels of the four genes (β-catenin, adenomatous polyposis coli (APC), and lipoprotein receptor-related proteins 5 and 6 (LRP5/6)). The synthesized nanoparticles were 25 nm on average and spherical in shape and aggregated form. Noteworthy cytotoxicity is how green-synthesized M. oleifera–AgNPs were observed in comparison with the M. oleifera leaf extract against a cancerous cell line. The M. oleifera–AgNPs decreased the expression of CTNNB1 and LRP6 genes, while the LRP5 gene expression increased in both cell lines. With treatment, the APC gene expression decreased in SW480 but increased in HTC116. Our results imply that AgNPs synthesized by M. oleifera extract could be an ideal strategy to combat colon cancer.https://doi.org/10.1515/gps-2022-0052moringa oleiferaplant extractnanosilveranticancer activity |
| spellingShingle | Althomali Arwa Daghestani Maha H. Basil Almukaynizi Fatimah Al-Zahrani Sabah Ahmed Awad Manal A. Merghani Nada M. Bukhari Wadha I. Ibrahim Eiman M. Alzahrani Sherifah M. Altowair Nouf AL-Ghamdi Afaf S. AlQahtani Asma M. Ramadan Rasha Bhat Ramesa Shafi Anti-colon cancer activities of green-synthesized Moringa oleifera–AgNPs against human colon cancer cells Green Processing and Synthesis moringa oleifera plant extract nanosilver anticancer activity |
| title | Anti-colon cancer activities of green-synthesized Moringa oleifera–AgNPs against human colon cancer cells |
| title_full | Anti-colon cancer activities of green-synthesized Moringa oleifera–AgNPs against human colon cancer cells |
| title_fullStr | Anti-colon cancer activities of green-synthesized Moringa oleifera–AgNPs against human colon cancer cells |
| title_full_unstemmed | Anti-colon cancer activities of green-synthesized Moringa oleifera–AgNPs against human colon cancer cells |
| title_short | Anti-colon cancer activities of green-synthesized Moringa oleifera–AgNPs against human colon cancer cells |
| title_sort | anti colon cancer activities of green synthesized moringa oleifera agnps against human colon cancer cells |
| topic | moringa oleifera plant extract nanosilver anticancer activity |
| url | https://doi.org/10.1515/gps-2022-0052 |
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