Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditions
Abstract Background Proline (Pro) and hydrogen peroxide (H2O2) play a critical role in plants during drought adaptation. Genetic mapping for drought-induced Pro and H2O2 production under field conditions is very limited in crop plants since their phenotyping with large populations is labor-intensive...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03943-9 |
| _version_ | 1811292453220646912 |
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| author | Mohammad Kamruzzaman Mekides Abebe Beyene Md Nurealam Siddiqui Agim Ballvora Jens Léon Ali Ahmad Naz |
| author_facet | Mohammad Kamruzzaman Mekides Abebe Beyene Md Nurealam Siddiqui Agim Ballvora Jens Léon Ali Ahmad Naz |
| author_sort | Mohammad Kamruzzaman |
| collection | DOAJ |
| description | Abstract Background Proline (Pro) and hydrogen peroxide (H2O2) play a critical role in plants during drought adaptation. Genetic mapping for drought-induced Pro and H2O2 production under field conditions is very limited in crop plants since their phenotyping with large populations is labor-intensive. A genome-wide association study (GWAS) of a diversity panel comprised of 184 bread wheat cultivars grown in natural field (control) and rain-out shelter (drought) environments was performed to identify candidate loci and genes regulating Pro and H2O2 accumulation induced by drought. Results The GWAS identified top significant marker-trait associations (MTAs) on 1A and 2A chromosomes, respectively for Pro and H2O2 in response to drought. Similarly, MTAs for stress tolerance index (STI) of Pro and H2O2 were identified on 5B and 1B chromosomes, respectively. Total 143 significant MTAs were identified including 36 and 71 were linked to drought and 2 and 34 were linked to STI for Pro and H2O2, respectively. Next, linkage disequilibrium analysis revealed minor alleles of significant single-markers and haplotypes were associated with higher Pro and H2O2 accumulation under drought. Several putative candidate genes for Pro and H2O2 content encode proteins with kinase, transporter or protein-binding activities. Conclusions The identified genetic factors associated with Pro and H2O2 biosynthesis underlying drought adaptation lay a fundamental basis for functional studies and future marker-assisted breeding programs. |
| first_indexed | 2024-04-13T04:45:40Z |
| format | Article |
| id | doaj.art-04600697ae484a3a8b6eafade0f2fe1d |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T04:45:40Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-04600697ae484a3a8b6eafade0f2fe1d2022-12-22T03:01:51ZengBMCBMC Plant Biology1471-22292022-12-0122111810.1186/s12870-022-03943-9Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditionsMohammad Kamruzzaman0Mekides Abebe Beyene1Md Nurealam Siddiqui2Agim Ballvora3Jens Léon4Ali Ahmad Naz5Institute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of BonnInstitute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of BonnInstitute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of BonnInstitute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of BonnInstitute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of BonnInstitute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of BonnAbstract Background Proline (Pro) and hydrogen peroxide (H2O2) play a critical role in plants during drought adaptation. Genetic mapping for drought-induced Pro and H2O2 production under field conditions is very limited in crop plants since their phenotyping with large populations is labor-intensive. A genome-wide association study (GWAS) of a diversity panel comprised of 184 bread wheat cultivars grown in natural field (control) and rain-out shelter (drought) environments was performed to identify candidate loci and genes regulating Pro and H2O2 accumulation induced by drought. Results The GWAS identified top significant marker-trait associations (MTAs) on 1A and 2A chromosomes, respectively for Pro and H2O2 in response to drought. Similarly, MTAs for stress tolerance index (STI) of Pro and H2O2 were identified on 5B and 1B chromosomes, respectively. Total 143 significant MTAs were identified including 36 and 71 were linked to drought and 2 and 34 were linked to STI for Pro and H2O2, respectively. Next, linkage disequilibrium analysis revealed minor alleles of significant single-markers and haplotypes were associated with higher Pro and H2O2 accumulation under drought. Several putative candidate genes for Pro and H2O2 content encode proteins with kinase, transporter or protein-binding activities. Conclusions The identified genetic factors associated with Pro and H2O2 biosynthesis underlying drought adaptation lay a fundamental basis for functional studies and future marker-assisted breeding programs.https://doi.org/10.1186/s12870-022-03943-9DroughtProlineHydrogen peroxideGWASWheat diversity |
| spellingShingle | Mohammad Kamruzzaman Mekides Abebe Beyene Md Nurealam Siddiqui Agim Ballvora Jens Léon Ali Ahmad Naz Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditions BMC Plant Biology Drought Proline Hydrogen peroxide GWAS Wheat diversity |
| title | Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditions |
| title_full | Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditions |
| title_fullStr | Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditions |
| title_full_unstemmed | Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditions |
| title_short | Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditions |
| title_sort | pinpointing genomic loci for drought induced proline and hydrogen peroxide accumulation in bread wheat under field conditions |
| topic | Drought Proline Hydrogen peroxide GWAS Wheat diversity |
| url | https://doi.org/10.1186/s12870-022-03943-9 |
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