Genome-Wide Identification and Expression Analysis of the <i>Dof</i> Transcription Factor in Annual Alfalfa <i>Medicago polymorpha</i>
The <i>Dof</i> transcription factor is a plant-specific transcription gene family that plays various biological functions in plant development and stress response. However, no relevant research has been conducted on <i>Medicago polymorpha</i>. Here, 36 <i>MpDof</i>...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/9/1831 |
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| author | Linghua Yang Xueyang Min Zhenwu Wei Nana Liu Jiaqing Li Youxin Zhang Yuwei Yang |
| author_facet | Linghua Yang Xueyang Min Zhenwu Wei Nana Liu Jiaqing Li Youxin Zhang Yuwei Yang |
| author_sort | Linghua Yang |
| collection | DOAJ |
| description | The <i>Dof</i> transcription factor is a plant-specific transcription gene family that plays various biological functions in plant development and stress response. However, no relevant research has been conducted on <i>Medicago polymorpha</i>. Here, 36 <i>MpDof</i> genes were identified in the <i>M. polymorpha</i> genome and further divided into 10 groups based on the comparative phylogenetic analysis. The essential information of <i>MpDof</i> genes, such as chromosomal localization, gene structure, conserved motifs, and selective pressures were systematically analyzed. All 36 <i>MpDof</i> genes were predicted to contain more <i>cis</i>-acting elements related to hormone response. <i>MpDof24</i> and <i>MpDof25</i> were predicted to interact with <i>MpDof11</i> and <i>MpDof26</i> to involve in the photoperiod blooms process. The <i>MpDof</i> genes showed a diverse expression pattern in different tissues. Notably, <i>MpDof29</i> and <i>MpDof31</i> were specifically expressed in the large pod and root, respectively, suggesting their crucial role in the pod and root development. qRT-PCR analysis indicated that the expression levels of <i>MpDof10</i>, <i>MpDof25</i>, <i>MpDof26</i>, and <i>MpDof29</i> were obviously up-regulated under drought, salt, and cold stress. Collectively, genome-wide identification, evolutionary, and expression analysis of the <i>Dof</i> transcription gene family in <i>M. polymorpha</i> will provide new information to further understand and utilize the function of these <i>Dof</i> genes in <i>Medicago</i> plants. |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:09:42Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-0460455918c640378546febc02feeba42023-11-17T23:32:21ZengMDPI AGPlants2223-77472023-04-01129183110.3390/plants12091831Genome-Wide Identification and Expression Analysis of the <i>Dof</i> Transcription Factor in Annual Alfalfa <i>Medicago polymorpha</i>Linghua Yang0Xueyang Min1Zhenwu Wei2Nana Liu3Jiaqing Li4Youxin Zhang5Yuwei Yang6College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaThe <i>Dof</i> transcription factor is a plant-specific transcription gene family that plays various biological functions in plant development and stress response. However, no relevant research has been conducted on <i>Medicago polymorpha</i>. Here, 36 <i>MpDof</i> genes were identified in the <i>M. polymorpha</i> genome and further divided into 10 groups based on the comparative phylogenetic analysis. The essential information of <i>MpDof</i> genes, such as chromosomal localization, gene structure, conserved motifs, and selective pressures were systematically analyzed. All 36 <i>MpDof</i> genes were predicted to contain more <i>cis</i>-acting elements related to hormone response. <i>MpDof24</i> and <i>MpDof25</i> were predicted to interact with <i>MpDof11</i> and <i>MpDof26</i> to involve in the photoperiod blooms process. The <i>MpDof</i> genes showed a diverse expression pattern in different tissues. Notably, <i>MpDof29</i> and <i>MpDof31</i> were specifically expressed in the large pod and root, respectively, suggesting their crucial role in the pod and root development. qRT-PCR analysis indicated that the expression levels of <i>MpDof10</i>, <i>MpDof25</i>, <i>MpDof26</i>, and <i>MpDof29</i> were obviously up-regulated under drought, salt, and cold stress. Collectively, genome-wide identification, evolutionary, and expression analysis of the <i>Dof</i> transcription gene family in <i>M. polymorpha</i> will provide new information to further understand and utilize the function of these <i>Dof</i> genes in <i>Medicago</i> plants.https://www.mdpi.com/2223-7747/12/9/1831<i>Medicago polymorpha</i><i>Dof</i> transcription factorbioinformaticsabiotic stressesgene expression |
| spellingShingle | Linghua Yang Xueyang Min Zhenwu Wei Nana Liu Jiaqing Li Youxin Zhang Yuwei Yang Genome-Wide Identification and Expression Analysis of the <i>Dof</i> Transcription Factor in Annual Alfalfa <i>Medicago polymorpha</i> Plants <i>Medicago polymorpha</i> <i>Dof</i> transcription factor bioinformatics abiotic stresses gene expression |
| title | Genome-Wide Identification and Expression Analysis of the <i>Dof</i> Transcription Factor in Annual Alfalfa <i>Medicago polymorpha</i> |
| title_full | Genome-Wide Identification and Expression Analysis of the <i>Dof</i> Transcription Factor in Annual Alfalfa <i>Medicago polymorpha</i> |
| title_fullStr | Genome-Wide Identification and Expression Analysis of the <i>Dof</i> Transcription Factor in Annual Alfalfa <i>Medicago polymorpha</i> |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of the <i>Dof</i> Transcription Factor in Annual Alfalfa <i>Medicago polymorpha</i> |
| title_short | Genome-Wide Identification and Expression Analysis of the <i>Dof</i> Transcription Factor in Annual Alfalfa <i>Medicago polymorpha</i> |
| title_sort | genome wide identification and expression analysis of the i dof i transcription factor in annual alfalfa i medicago polymorpha i |
| topic | <i>Medicago polymorpha</i> <i>Dof</i> transcription factor bioinformatics abiotic stresses gene expression |
| url | https://www.mdpi.com/2223-7747/12/9/1831 |
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