Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies
Shigella spp. and Escherichia coli are closely related; both belong to the family Enterobacteriaceae. Phenotypically, Shigella spp. and E. coli share many common characteristics, yet they have separate entities in epidemiology and clinical disease, which poses a diagnostic challenge. We collated inf...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | New Microbes and New Infections |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297517300744 |
| _version_ | 1811336550619807744 |
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| author | N.K. Devanga Ragupathi D.P. Muthuirulandi Sethuvel F.Y. Inbanathan B. Veeraraghavan |
| author_facet | N.K. Devanga Ragupathi D.P. Muthuirulandi Sethuvel F.Y. Inbanathan B. Veeraraghavan |
| author_sort | N.K. Devanga Ragupathi |
| collection | DOAJ |
| description | Shigella spp. and Escherichia coli are closely related; both belong to the family Enterobacteriaceae. Phenotypically, Shigella spp. and E. coli share many common characteristics, yet they have separate entities in epidemiology and clinical disease, which poses a diagnostic challenge. We collated information for the best possible approach to differentiate clinically relevant E. coli from Shigella spp. We found that a molecular approach is required for confirmation. High discriminatory potential is seen with whole genome sequencing analysed for k-mers and single nucleotide polymorphism. Among these, identification using single nucleotide polymorphism is easy to perform and analyse, and it thus appears more promising. Among the nonmolecular methods, matrix-assisted desorption ionization–time of flight mass spectrometry may be applicable when data analysis is assisted with advanced analytic tools. Keywords: 16S rRNA, k-mer, MALDI-TOF MS, single nucleotide polymorphism, whole genome sequencing |
| first_indexed | 2024-04-13T17:41:08Z |
| format | Article |
| id | doaj.art-0462508667814f0ba3a5e429e5f2c8bd |
| institution | Directory Open Access Journal |
| issn | 2052-2975 |
| language | English |
| last_indexed | 2024-04-13T17:41:08Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | New Microbes and New Infections |
| spelling | doaj.art-0462508667814f0ba3a5e429e5f2c8bd2022-12-22T02:37:10ZengElsevierNew Microbes and New Infections2052-29752018-01-01215862Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategiesN.K. Devanga Ragupathi0D.P. Muthuirulandi Sethuvel1F.Y. Inbanathan2B. Veeraraghavan3Department of Clinical Microbiology, Christian Medical College, Vellore, IndiaDepartment of Clinical Microbiology, Christian Medical College, Vellore, IndiaDepartment of Clinical Microbiology, Christian Medical College, Vellore, IndiaCorresponding author. B. Veeraraghavan, Department of Clinical Microbiology, Christian Medical College, Vellore 4, India; Department of Clinical Microbiology, Christian Medical College, Vellore, IndiaShigella spp. and Escherichia coli are closely related; both belong to the family Enterobacteriaceae. Phenotypically, Shigella spp. and E. coli share many common characteristics, yet they have separate entities in epidemiology and clinical disease, which poses a diagnostic challenge. We collated information for the best possible approach to differentiate clinically relevant E. coli from Shigella spp. We found that a molecular approach is required for confirmation. High discriminatory potential is seen with whole genome sequencing analysed for k-mers and single nucleotide polymorphism. Among these, identification using single nucleotide polymorphism is easy to perform and analyse, and it thus appears more promising. Among the nonmolecular methods, matrix-assisted desorption ionization–time of flight mass spectrometry may be applicable when data analysis is assisted with advanced analytic tools. Keywords: 16S rRNA, k-mer, MALDI-TOF MS, single nucleotide polymorphism, whole genome sequencinghttp://www.sciencedirect.com/science/article/pii/S2052297517300744 |
| spellingShingle | N.K. Devanga Ragupathi D.P. Muthuirulandi Sethuvel F.Y. Inbanathan B. Veeraraghavan Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies New Microbes and New Infections |
| title | Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies |
| title_full | Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies |
| title_fullStr | Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies |
| title_full_unstemmed | Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies |
| title_short | Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies |
| title_sort | accurate differentiation of escherichia coli and shigella serogroups challenges and strategies |
| url | http://www.sciencedirect.com/science/article/pii/S2052297517300744 |
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