Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells
Probiotics in livestock feed supplements are considered a replacement for antibiotics that enhance gastrointestinal immunity. Although bacterial cell wall components have been proposed to be associated with probiotic function, little evidence demonstrates that they are responsible for probiotic func...
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| Format: | Article |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01827/full |
| _version_ | 1818082931513491456 |
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| author | Kyoung Whun Kim Kyoung Whun Kim Seok-Seong Kang Sun-Je Woo Ok-Jin Park Ki Bum Ahn Ki-Duk Song Hak-Kyo Lee Cheol-Heui Yun Cheol-Heui Yun Seung Hyun Han |
| author_facet | Kyoung Whun Kim Kyoung Whun Kim Seok-Seong Kang Sun-Je Woo Ok-Jin Park Ki Bum Ahn Ki-Duk Song Hak-Kyo Lee Cheol-Heui Yun Cheol-Heui Yun Seung Hyun Han |
| author_sort | Kyoung Whun Kim |
| collection | DOAJ |
| description | Probiotics in livestock feed supplements are considered a replacement for antibiotics that enhance gastrointestinal immunity. Although bacterial cell wall components have been proposed to be associated with probiotic function, little evidence demonstrates that they are responsible for probiotic functions in livestock. The present study demonstrated that lipoteichoic acid (LTA) of Lactobacillus plantarum (Lp.LTA) confers anti-inflammatory responses in porcine intestinal epithelial cell line, IPEC-J2. A synthetic analog of viral double-stranded RNA, poly I:C, dose-dependently induced IL-8 production at the mRNA and protein levels in IPEC-J2 cells. Lp.LTA, but not lipoprotein or peptidoglycan from L. plantarum, exclusively suppressed poly I:C-induced IL-8 production. Compared with LTAs from other probiotic Lactobacillus strains including L. delbrueckii, L. sakei, and L. rhamnosus GG, Lp.LTA had higher potential to suppress poly I:C-induced IL-8 production. Dealanylated or deacylated Lp.LTA did not suppress poly I:C-induced IL-8 production, suggesting that D-alanine and lipid moieties in the Lp.LTA structure were responsible for the inhibition. Furthermore, Lp.LTA attenuated the phosphorylation of ERK and p38 kinase as well as the activation of NF-κB, resulting in decreased IL-8 production. Taken together, these results suggest that Lp.LTA acts as an effector molecule to inhibit viral pathogen-induced inflammatory responses in porcine intestinal epithelial cells. |
| first_indexed | 2024-12-10T19:29:56Z |
| format | Article |
| id | doaj.art-04628445e3fe4f4db85ad53951ec148d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T19:29:56Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-04628445e3fe4f4db85ad53951ec148d2022-12-22T01:36:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-09-01810.3389/fmicb.2017.01827297380Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial CellsKyoung Whun Kim0Kyoung Whun Kim1Seok-Seong Kang2Sun-Je Woo3Ok-Jin Park4Ki Bum Ahn5Ki-Duk Song6Hak-Kyo Lee7Cheol-Heui Yun8Cheol-Heui Yun9Seung Hyun Han10Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National UniversitySeoul, South KoreaDepartment of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National UniversitySeoul, South KoreaDepartment of Food Science and Biotechnology, Dongguk University-SeoulSeoul, South KoreaDepartment of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National UniversitySeoul, South KoreaDepartment of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National UniversitySeoul, South KoreaDepartment of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National UniversitySeoul, South KoreaDepartment of Animal Biotechnology, Chonbuk National UniversityJeonju, South KoreaDepartment of Animal Biotechnology, Chonbuk National UniversityJeonju, South KoreaDepartment of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National UniversitySeoul, South KoreaInstitute of Green Bio Science Technology, Seoul National UniversitySeoul, South KoreaDepartment of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National UniversitySeoul, South KoreaProbiotics in livestock feed supplements are considered a replacement for antibiotics that enhance gastrointestinal immunity. Although bacterial cell wall components have been proposed to be associated with probiotic function, little evidence demonstrates that they are responsible for probiotic functions in livestock. The present study demonstrated that lipoteichoic acid (LTA) of Lactobacillus plantarum (Lp.LTA) confers anti-inflammatory responses in porcine intestinal epithelial cell line, IPEC-J2. A synthetic analog of viral double-stranded RNA, poly I:C, dose-dependently induced IL-8 production at the mRNA and protein levels in IPEC-J2 cells. Lp.LTA, but not lipoprotein or peptidoglycan from L. plantarum, exclusively suppressed poly I:C-induced IL-8 production. Compared with LTAs from other probiotic Lactobacillus strains including L. delbrueckii, L. sakei, and L. rhamnosus GG, Lp.LTA had higher potential to suppress poly I:C-induced IL-8 production. Dealanylated or deacylated Lp.LTA did not suppress poly I:C-induced IL-8 production, suggesting that D-alanine and lipid moieties in the Lp.LTA structure were responsible for the inhibition. Furthermore, Lp.LTA attenuated the phosphorylation of ERK and p38 kinase as well as the activation of NF-κB, resulting in decreased IL-8 production. Taken together, these results suggest that Lp.LTA acts as an effector molecule to inhibit viral pathogen-induced inflammatory responses in porcine intestinal epithelial cells.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01827/fullprobioticslipoteichoic acidviral infectioninflammationintestinal epithelial cells |
| spellingShingle | Kyoung Whun Kim Kyoung Whun Kim Seok-Seong Kang Sun-Je Woo Ok-Jin Park Ki Bum Ahn Ki-Duk Song Hak-Kyo Lee Cheol-Heui Yun Cheol-Heui Yun Seung Hyun Han Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells Frontiers in Microbiology probiotics lipoteichoic acid viral infection inflammation intestinal epithelial cells |
| title | Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells |
| title_full | Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells |
| title_fullStr | Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells |
| title_full_unstemmed | Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells |
| title_short | Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells |
| title_sort | lipoteichoic acid of probiotic lactobacillus plantarum attenuates poly i c induced il 8 production in porcine intestinal epithelial cells |
| topic | probiotics lipoteichoic acid viral infection inflammation intestinal epithelial cells |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01827/full |
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