Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean
Recently, climate change has had an increasing impact on the world. Innate defense mechanisms operating in plants - such as PAMP-triggered Immunity (PTI) - help to reduce the adverse effects caused by various abiotic and biotic stressors. In this study, the effects of ELICE16INDURES® plant condition...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023001147 |
| _version_ | 1811173321498165248 |
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| author | Kincső Decsi Barbara Kutasy Géza Hegedűs Zoltán Péter Alföldi Nikoletta Kálmán Ágnes Nagy Eszter Virág |
| author_facet | Kincső Decsi Barbara Kutasy Géza Hegedűs Zoltán Péter Alföldi Nikoletta Kálmán Ágnes Nagy Eszter Virág |
| author_sort | Kincső Decsi |
| collection | DOAJ |
| description | Recently, climate change has had an increasing impact on the world. Innate defense mechanisms operating in plants - such as PAMP-triggered Immunity (PTI) - help to reduce the adverse effects caused by various abiotic and biotic stressors. In this study, the effects of ELICE16INDURES® plant conditioner for organic farming, developed by the Research Institute for Medicinal Plants and Herbs Ltd. Budakalász Hungary, were studied in a soybean population in Northern Hungary. The active compounds and ingredients of this product were selected in such a way as to facilitate the triggering of general plant immunity without the presence and harmful effects of pathogens, thereby strengthening the healthy plant population and preparing it for possible stress effects. In practice, treatments of this agent were applied at two different time points and two concentrations. The conditioning effect was well demonstrated by using agro-drone and ENDVI determination in the soybean field. The genetic background of healthier plants was investigated by NGS sequencing, and by the expression levels of genes encoding enzymes involved in the catalysis of metabolic pathways regulating PTI. The genome-wide transcriptional profiling resulted in 13 contigs related to PAMP-triggered immunity and activated as a result of the treatments. Further analyses showed 16 additional PTI-related contigs whose gene expression changed positively as a result of the treatments. The gene expression values of genes encoded in these contigs were determined by in silico mRNA quantification and validated by RT-qPCR. Both - relatively low and high treatments - showed an increase in gene expression of key genes involving AOC, IFS, MAPK4, MEKK, and GST. Transcriptomic results indicated that the biosyntheses of jasmonic acid (JA), salicylic acid (SA), phenylpropanoid, flavonoid, phytoalexin, and cellular detoxification processes were triggered in the appropriate molecular steps and suggested that plant immune reactions may be activated also artificially, and innate immunity can be enhanced with proper plant biostimulants. |
| first_indexed | 2024-04-10T17:44:57Z |
| format | Article |
| id | doaj.art-04654307dd4149ad9d602984a46ad7a0 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T17:44:57Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-04654307dd4149ad9d602984a46ad7a02023-02-03T04:59:39ZengElsevierHeliyon2405-84402023-01-0191e12907Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybeanKincső Decsi0Barbara Kutasy1Géza Hegedűs2Zoltán Péter Alföldi3Nikoletta Kálmán4Ágnes Nagy5Eszter Virág6Department of Plant Physiology and Plant Ecology, Campus Keszthely, Hungarian University of Agriculture and Life Sciences Georgikon, Keszthely, HungaryDepartment of Plant Physiology and Plant Ecology, Campus Keszthely, Hungarian University of Agriculture and Life Sciences Georgikon, Keszthely, Hungary; Corresponding author.EduCoMat Ltd., Keszthely, Hungary; Department of Information Technology and Its Applications, Faculty of Information Technology, University of Pannonia, Zalaegerszeg, Hungary; Institute of Metagenomics, University of Debrecen, Debrecen, HungaryDepartment of Environmental Biology, Campus Keszthely, Hungarian University of Agriculture and Life Sciences Georgikon, Keszthely, HungaryDepartment of Biochemistry and Medical Chemistry, University of Pecs, Medical School, Pecs, HungaryResearch Institute for Medicinal Plants and Herbs Ltd., Budakalász, HungaryEduCoMat Ltd., Keszthely, Hungary; Institute of Metagenomics, University of Debrecen, Debrecen, Hungary; Research Institute for Medicinal Plants and Herbs Ltd., Budakalász, Hungary; Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, Debrecen, HungaryRecently, climate change has had an increasing impact on the world. Innate defense mechanisms operating in plants - such as PAMP-triggered Immunity (PTI) - help to reduce the adverse effects caused by various abiotic and biotic stressors. In this study, the effects of ELICE16INDURES® plant conditioner for organic farming, developed by the Research Institute for Medicinal Plants and Herbs Ltd. Budakalász Hungary, were studied in a soybean population in Northern Hungary. The active compounds and ingredients of this product were selected in such a way as to facilitate the triggering of general plant immunity without the presence and harmful effects of pathogens, thereby strengthening the healthy plant population and preparing it for possible stress effects. In practice, treatments of this agent were applied at two different time points and two concentrations. The conditioning effect was well demonstrated by using agro-drone and ENDVI determination in the soybean field. The genetic background of healthier plants was investigated by NGS sequencing, and by the expression levels of genes encoding enzymes involved in the catalysis of metabolic pathways regulating PTI. The genome-wide transcriptional profiling resulted in 13 contigs related to PAMP-triggered immunity and activated as a result of the treatments. Further analyses showed 16 additional PTI-related contigs whose gene expression changed positively as a result of the treatments. The gene expression values of genes encoded in these contigs were determined by in silico mRNA quantification and validated by RT-qPCR. Both - relatively low and high treatments - showed an increase in gene expression of key genes involving AOC, IFS, MAPK4, MEKK, and GST. Transcriptomic results indicated that the biosyntheses of jasmonic acid (JA), salicylic acid (SA), phenylpropanoid, flavonoid, phytoalexin, and cellular detoxification processes were triggered in the appropriate molecular steps and suggested that plant immune reactions may be activated also artificially, and innate immunity can be enhanced with proper plant biostimulants.http://www.sciencedirect.com/science/article/pii/S2405844023001147ELICE16INDURES®Expression profilingDefense mechanismSoybeanGlycine max |
| spellingShingle | Kincső Decsi Barbara Kutasy Géza Hegedűs Zoltán Péter Alföldi Nikoletta Kálmán Ágnes Nagy Eszter Virág Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean Heliyon ELICE16INDURES® Expression profiling Defense mechanism Soybean Glycine max |
| title | Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean |
| title_full | Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean |
| title_fullStr | Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean |
| title_full_unstemmed | Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean |
| title_short | Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean |
| title_sort | natural immunity stimulation using elice16indures r plant conditioner in field culture of soybean |
| topic | ELICE16INDURES® Expression profiling Defense mechanism Soybean Glycine max |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023001147 |
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