Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways
While SlPti5 has been shown to play a crucial role in the regulation of antagonistic genes in Solanum lycopersicum and Arabidopsis against pathogen infection, there have been no comprehensive studies on the effects of SlPti5 on the regulatory response mechanism of reactive oxygen species (ROS) syste...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921636304 |
| _version_ | 1798024113120346112 |
|---|---|
| author | Qiong TANG Xiao-dong ZHENG Jun GUO Ting YU |
| author_facet | Qiong TANG Xiao-dong ZHENG Jun GUO Ting YU |
| author_sort | Qiong TANG |
| collection | DOAJ |
| description | While SlPti5 has been shown to play a crucial role in the regulation of antagonistic genes in Solanum lycopersicum and Arabidopsis against pathogen infection, there have been no comprehensive studies on the effects of SlPti5 on the regulatory response mechanism of reactive oxygen species (ROS) system and hormone pathways during growth and disease resistance of tomato plants. Here, we investigated the function of SlPti5 in the defense response of tomato against Botrytis cinerea utilizing a virus-induced gene silencing (VIGS)-based system. Expression profile analysis showed that SlPti5 was significantly induced upon B. cinerea infection, with high expression levels in the leaves and fruit of tomato. VIGS-based silencing of SlPti5 inhibited early vegetative growth, increased the plant's susceptibility to infection, promoted the development of ROS, affected the expression of genes involved in the ROS scavenging system, and attenuated the expression of genes associated with pathogenesis and the ethylene/jasmonic acid signaling pathways. In sum, our data demonstrated that SlPti5 stimulates the immune response of tomato plant to Botrytis cinerea infection by involving the ethylene (ET)- and jasmonic acid (JA)-mediated pathways and modulating the expression of some key pathogenesis-related (PR) genes. |
| first_indexed | 2024-04-11T17:58:16Z |
| format | Article |
| id | doaj.art-0466f30d3aa34e7e933c0eaacd54ffd6 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-11T17:58:16Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-0466f30d3aa34e7e933c0eaacd54ffd62022-12-22T04:10:36ZengElsevierJournal of Integrative Agriculture2095-31192022-03-01213697709Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathwaysQiong TANG0Xiao-dong ZHENG1Jun GUO2Ting YU3College of Biosystems Engineering and Food Science, National Engineering Laboratory of Intelligent Food Technology and Equipment/Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture/Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, P.R.ChinaCollege of Biosystems Engineering and Food Science, National Engineering Laboratory of Intelligent Food Technology and Equipment/Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture/Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, P.R.ChinaState Key Laboratory of Food Nutrition and Safety/Key Laboratory of Food Nutrition and Safety, Ministry of Education/Key Laboratory of Food Quality and Health of Tianjin, Tianjin University of Science and Technology, Tianjin 300457, P.R.China; Correspondence GUO Jun, Tel/Fax: +86-22-60912486College of Biosystems Engineering and Food Science, National Engineering Laboratory of Intelligent Food Technology and Equipment/Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture/Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, P.R.China; Correspondence YU Ting, Tel/Fax: +86-571-88982191While SlPti5 has been shown to play a crucial role in the regulation of antagonistic genes in Solanum lycopersicum and Arabidopsis against pathogen infection, there have been no comprehensive studies on the effects of SlPti5 on the regulatory response mechanism of reactive oxygen species (ROS) system and hormone pathways during growth and disease resistance of tomato plants. Here, we investigated the function of SlPti5 in the defense response of tomato against Botrytis cinerea utilizing a virus-induced gene silencing (VIGS)-based system. Expression profile analysis showed that SlPti5 was significantly induced upon B. cinerea infection, with high expression levels in the leaves and fruit of tomato. VIGS-based silencing of SlPti5 inhibited early vegetative growth, increased the plant's susceptibility to infection, promoted the development of ROS, affected the expression of genes involved in the ROS scavenging system, and attenuated the expression of genes associated with pathogenesis and the ethylene/jasmonic acid signaling pathways. In sum, our data demonstrated that SlPti5 stimulates the immune response of tomato plant to Botrytis cinerea infection by involving the ethylene (ET)- and jasmonic acid (JA)-mediated pathways and modulating the expression of some key pathogenesis-related (PR) genes.http://www.sciencedirect.com/science/article/pii/S2095311921636304tomato (Solanum lycopersicum)Botrytis cinereaSlPti5immune responseET- and JA-mediated signaling pathwayspathogenesis-related proteins |
| spellingShingle | Qiong TANG Xiao-dong ZHENG Jun GUO Ting YU Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways Journal of Integrative Agriculture tomato (Solanum lycopersicum) Botrytis cinerea SlPti5 immune response ET- and JA-mediated signaling pathways pathogenesis-related proteins |
| title | Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways |
| title_full | Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways |
| title_fullStr | Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways |
| title_full_unstemmed | Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways |
| title_short | Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways |
| title_sort | tomato slpti5 plays a regulative role in the plant immune response against botrytis cinerea through modulation of ros system and hormone pathways |
| topic | tomato (Solanum lycopersicum) Botrytis cinerea SlPti5 immune response ET- and JA-mediated signaling pathways pathogenesis-related proteins |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921636304 |
| work_keys_str_mv | AT qiongtang tomatoslpti5playsaregulativeroleintheplantimmuneresponseagainstbotrytiscinereathroughmodulationofrossystemandhormonepathways AT xiaodongzheng tomatoslpti5playsaregulativeroleintheplantimmuneresponseagainstbotrytiscinereathroughmodulationofrossystemandhormonepathways AT junguo tomatoslpti5playsaregulativeroleintheplantimmuneresponseagainstbotrytiscinereathroughmodulationofrossystemandhormonepathways AT tingyu tomatoslpti5playsaregulativeroleintheplantimmuneresponseagainstbotrytiscinereathroughmodulationofrossystemandhormonepathways |