Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes
Single-molecule force spectroscopy with the atomic force microscope provides molecular level insights into protein function, allowing researchers to reconstruct energy landscapes and understand functional mechanisms in biology. With steadily advancing methods, this technique has greatly accelerated...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-05-01
|
| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmolb.2020.00085/full |
| _version_ | 1818598248418377728 |
|---|---|
| author | Byeongseon Yang Byeongseon Yang Zhaowei Liu Zhaowei Liu Haipei Liu Haipei Liu Michael A. Nash Michael A. Nash |
| author_facet | Byeongseon Yang Byeongseon Yang Zhaowei Liu Zhaowei Liu Haipei Liu Haipei Liu Michael A. Nash Michael A. Nash |
| author_sort | Byeongseon Yang |
| collection | DOAJ |
| description | Single-molecule force spectroscopy with the atomic force microscope provides molecular level insights into protein function, allowing researchers to reconstruct energy landscapes and understand functional mechanisms in biology. With steadily advancing methods, this technique has greatly accelerated our understanding of force transduction, mechanical deformation, and mechanostability within single- and multi-domain polyproteins, and receptor-ligand complexes. In this focused review, we summarize the state of the art in terms of methodology and highlight recent methodological improvements for AFM-SMFS experiments, including developments in surface chemistry, considerations for protein engineering, as well as theory and algorithms for data analysis. We hope that by condensing and disseminating these methods, they can assist the community in improving data yield, reliability, and throughput and thereby enhance the information that researchers can extract from such experiments. These leading edge methods for AFM-SMFS will serve as a groundwork for researchers cognizant of its current limitations who seek to improve the technique in the future for in-depth studies of molecular biomechanics. |
| first_indexed | 2024-12-16T12:00:41Z |
| format | Article |
| id | doaj.art-046b1dc55e6d4b36aa65c3eb6570f27d |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-16T12:00:41Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-046b1dc55e6d4b36aa65c3eb6570f27d2022-12-21T22:32:27ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2020-05-01710.3389/fmolb.2020.00085537771Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand ComplexesByeongseon Yang0Byeongseon Yang1Zhaowei Liu2Zhaowei Liu3Haipei Liu4Haipei Liu5Michael A. Nash6Michael A. Nash7Department of Chemistry, University of Basel, Basel, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, SwitzerlandDepartment of Chemistry, University of Basel, Basel, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, SwitzerlandDepartment of Chemistry, University of Basel, Basel, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, SwitzerlandDepartment of Chemistry, University of Basel, Basel, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, SwitzerlandSingle-molecule force spectroscopy with the atomic force microscope provides molecular level insights into protein function, allowing researchers to reconstruct energy landscapes and understand functional mechanisms in biology. With steadily advancing methods, this technique has greatly accelerated our understanding of force transduction, mechanical deformation, and mechanostability within single- and multi-domain polyproteins, and receptor-ligand complexes. In this focused review, we summarize the state of the art in terms of methodology and highlight recent methodological improvements for AFM-SMFS experiments, including developments in surface chemistry, considerations for protein engineering, as well as theory and algorithms for data analysis. We hope that by condensing and disseminating these methods, they can assist the community in improving data yield, reliability, and throughput and thereby enhance the information that researchers can extract from such experiments. These leading edge methods for AFM-SMFS will serve as a groundwork for researchers cognizant of its current limitations who seek to improve the technique in the future for in-depth studies of molecular biomechanics.https://www.frontiersin.org/article/10.3389/fmolb.2020.00085/fullsingle-molecule biophysicsmolecular engineeringAFMprotein stability and foldingmolecular biomechanics |
| spellingShingle | Byeongseon Yang Byeongseon Yang Zhaowei Liu Zhaowei Liu Haipei Liu Haipei Liu Michael A. Nash Michael A. Nash Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes Frontiers in Molecular Biosciences single-molecule biophysics molecular engineering AFM protein stability and folding molecular biomechanics |
| title | Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes |
| title_full | Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes |
| title_fullStr | Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes |
| title_full_unstemmed | Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes |
| title_short | Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes |
| title_sort | next generation methods for single molecule force spectroscopy on polyproteins and receptor ligand complexes |
| topic | single-molecule biophysics molecular engineering AFM protein stability and folding molecular biomechanics |
| url | https://www.frontiersin.org/article/10.3389/fmolb.2020.00085/full |
| work_keys_str_mv | AT byeongseonyang nextgenerationmethodsforsinglemoleculeforcespectroscopyonpolyproteinsandreceptorligandcomplexes AT byeongseonyang nextgenerationmethodsforsinglemoleculeforcespectroscopyonpolyproteinsandreceptorligandcomplexes AT zhaoweiliu nextgenerationmethodsforsinglemoleculeforcespectroscopyonpolyproteinsandreceptorligandcomplexes AT zhaoweiliu nextgenerationmethodsforsinglemoleculeforcespectroscopyonpolyproteinsandreceptorligandcomplexes AT haipeiliu nextgenerationmethodsforsinglemoleculeforcespectroscopyonpolyproteinsandreceptorligandcomplexes AT haipeiliu nextgenerationmethodsforsinglemoleculeforcespectroscopyonpolyproteinsandreceptorligandcomplexes AT michaelanash nextgenerationmethodsforsinglemoleculeforcespectroscopyonpolyproteinsandreceptorligandcomplexes AT michaelanash nextgenerationmethodsforsinglemoleculeforcespectroscopyonpolyproteinsandreceptorligandcomplexes |