Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer
Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer (LC). This list includes EFEMP...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2019-09-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2019.09.01 |
| _version_ | 1811205613522255872 |
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| author | N.A. Smetannikova A.A. Evdokimov N.A. Netesova M.A. Abdurashitov A.G. Akishev E.V. Dubinin P.I. Pozdnyakov I.V. Vihlyanov M.K. Nikitin E.B. Topolnitsky A.B. Karpov S.A. Kolomiets S.Kh. Degtyarev |
| author_facet | N.A. Smetannikova A.A. Evdokimov N.A. Netesova M.A. Abdurashitov A.G. Akishev E.V. Dubinin P.I. Pozdnyakov I.V. Vihlyanov M.K. Nikitin E.B. Topolnitsky A.B. Karpov S.A. Kolomiets S.Kh. Degtyarev |
| author_sort | N.A. Smetannikova |
| collection | DOAJ |
| description | Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer (LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1A and TERT genes with relatively high sensitivity (80.0 %) and specificity (88.0 %) of LC detection in tumor DNA. |
| first_indexed | 2024-04-12T03:33:59Z |
| format | Article |
| id | doaj.art-046b7220afcd426dbcd722ab89ef5e7a |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-04-12T03:33:59Z |
| publishDate | 2019-09-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-046b7220afcd426dbcd722ab89ef5e7a2022-12-22T03:49:28ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872019-09-0122955156110.3779/j.issn.1009-3419.2019.09.01Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung CancerN.A. Smetannikova0A.A. Evdokimov1N.A. Netesova2M.A. Abdurashitov3A.G. Akishev4E.V. Dubinin5P.I. Pozdnyakov6I.V. Vihlyanov7M.K. Nikitin8E.B. Topolnitsky9A.B. Karpov10S.A. Kolomiets11S.Kh. Degtyarev12State Research Center of Virology and Biotechnology, Koltsovo, RussiaState Research Center of Virology and Biotechnology, Koltsovo, RussiaState Research Center of Virology and Biotechnology, Koltsovo, RussiaEpiGene LLC, Novosibirsk, RussiaEpiGene LLC, Novosibirsk, RussiaEpiGene LLC, Novosibirsk, RussiaState Research Center of Virology and Biotechnology, Koltsovo, RussiaAltai Regional Oncology Center, Barnaul, RussiaAltai Regional Oncology Center, Barnaul, RussiaTomsk Regional Clinical Hospital, Tomsk, RussiaSeversk Biophysical Research Centre, Seversk, RussiaRegional Clinical Oncology Center, Kemerovo, RussiaEpiGene LLC, Novosibirsk, RussiaHypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer (LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1A and TERT genes with relatively high sensitivity (80.0 %) and specificity (88.0 %) of LC detection in tumor DNA.http://dx.doi.org/10.3779/j.issn.1009-3419.2019.09.01Lung neoplasmsDNA methylationGLAD-PCRDiagnosticsEpigenetics |
| spellingShingle | N.A. Smetannikova A.A. Evdokimov N.A. Netesova M.A. Abdurashitov A.G. Akishev E.V. Dubinin P.I. Pozdnyakov I.V. Vihlyanov M.K. Nikitin E.B. Topolnitsky A.B. Karpov S.A. Kolomiets S.Kh. Degtyarev Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer Chinese Journal of Lung Cancer Lung neoplasms DNA methylation GLAD-PCR Diagnostics Epigenetics |
| title | Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer |
| title_full | Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer |
| title_fullStr | Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer |
| title_full_unstemmed | Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer |
| title_short | Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer |
| title_sort | application of glad pcr assay for study on dna methylation in regulatory regions of some tumor suppressor genes in lung cancer |
| topic | Lung neoplasms DNA methylation GLAD-PCR Diagnostics Epigenetics |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2019.09.01 |
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