Using publicly available datasets to identify population-based transcriptomic landscape contributing to the aggressiveness of breast cancer in young women
Introduction: Although the risk of breast cancer increases with advancing age, some regions have larger number of young breast cancer patients (≤45 years-old), such as the Middle East, Eastern Asia, and North Africa, with more aggressive and poorly differentiated tumors. We aimed to conduct an in-si...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.1039037/full |
| _version_ | 1797962249475719168 |
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| author | Marah Tabbal Mahmood Y. Hachim Reem Kais Jan Thomas E. Adrian |
| author_facet | Marah Tabbal Mahmood Y. Hachim Reem Kais Jan Thomas E. Adrian |
| author_sort | Marah Tabbal |
| collection | DOAJ |
| description | Introduction: Although the risk of breast cancer increases with advancing age, some regions have larger number of young breast cancer patients (≤45 years-old), such as the Middle East, Eastern Asia, and North Africa, with more aggressive and poorly differentiated tumors. We aimed to conduct an in-silico analysis in an attempt to understand the aggressive nature of early-onset breast cancer, and to identify potential drivers of early-onset breast cancer using gene expression profiling datasets in a population-dependent manner.Methods: Functional genomics experiments data were acquired from cBioPortal database for cancer genomics, followed by the stratification of patients based on the age at representation of breast cancer and race. Differential gene expression analysis and gene amplification status analysis were carried out, followed by hub gene, transcription factor, and signalling pathway identification.Results: PAM50 subtype analysis revealed that young patients (≤45 years-old) had four-fold more basal tumors and worst progression-free survival (median of 101 months), compared with the 45–65 years group (median of 168 months). Fourteen genes were amplified in more than 14% of patients with an early-onset breast cancer. Interestingly, FREM2, LINC00332, and LINC00366 were exclusively amplified in younger patients. Gene expression data from three different populations (Asian, White, and African) revealed a unique transcriptomic profile of young patients, which was also reflected on the PAM50 subtype analysis. Our data indicates a higher tendency of young African patients to develop basal tumors, while young Asian patients are more prone to developing Luminal A tumors. Most genes that were found to be upregulated in younger patients are involved in important signaling pathways that promote cancer progression and metastasis, such as MAPK pathway, Reelin pathway and the PI3K/Akt pathway.Conclusion: This study provides strong evidence that the molecular profile of tumors derived from young breast cancer patients of different populations is unique and may explain the aggressiveness of these tumors, stressing the need to conduct population- based multi-omic analyses to identify the potential drivers for tumorigenesis and molecular profiles of young breast cancer patients. |
| first_indexed | 2024-04-11T01:10:25Z |
| format | Article |
| id | doaj.art-046c3f21de4d479f870f0cad67aeac93 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T01:10:25Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-046c3f21de4d479f870f0cad67aeac932023-01-04T06:28:08ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-01-011310.3389/fgene.2022.10390371039037Using publicly available datasets to identify population-based transcriptomic landscape contributing to the aggressiveness of breast cancer in young womenMarah TabbalMahmood Y. HachimReem Kais JanThomas E. AdrianIntroduction: Although the risk of breast cancer increases with advancing age, some regions have larger number of young breast cancer patients (≤45 years-old), such as the Middle East, Eastern Asia, and North Africa, with more aggressive and poorly differentiated tumors. We aimed to conduct an in-silico analysis in an attempt to understand the aggressive nature of early-onset breast cancer, and to identify potential drivers of early-onset breast cancer using gene expression profiling datasets in a population-dependent manner.Methods: Functional genomics experiments data were acquired from cBioPortal database for cancer genomics, followed by the stratification of patients based on the age at representation of breast cancer and race. Differential gene expression analysis and gene amplification status analysis were carried out, followed by hub gene, transcription factor, and signalling pathway identification.Results: PAM50 subtype analysis revealed that young patients (≤45 years-old) had four-fold more basal tumors and worst progression-free survival (median of 101 months), compared with the 45–65 years group (median of 168 months). Fourteen genes were amplified in more than 14% of patients with an early-onset breast cancer. Interestingly, FREM2, LINC00332, and LINC00366 were exclusively amplified in younger patients. Gene expression data from three different populations (Asian, White, and African) revealed a unique transcriptomic profile of young patients, which was also reflected on the PAM50 subtype analysis. Our data indicates a higher tendency of young African patients to develop basal tumors, while young Asian patients are more prone to developing Luminal A tumors. Most genes that were found to be upregulated in younger patients are involved in important signaling pathways that promote cancer progression and metastasis, such as MAPK pathway, Reelin pathway and the PI3K/Akt pathway.Conclusion: This study provides strong evidence that the molecular profile of tumors derived from young breast cancer patients of different populations is unique and may explain the aggressiveness of these tumors, stressing the need to conduct population- based multi-omic analyses to identify the potential drivers for tumorigenesis and molecular profiles of young breast cancer patients.https://www.frontiersin.org/articles/10.3389/fgene.2022.1039037/fullearly-onsetbreast canceraggressivemetastasisgenomicsamplification |
| spellingShingle | Marah Tabbal Mahmood Y. Hachim Reem Kais Jan Thomas E. Adrian Using publicly available datasets to identify population-based transcriptomic landscape contributing to the aggressiveness of breast cancer in young women Frontiers in Genetics early-onset breast cancer aggressive metastasis genomics amplification |
| title | Using publicly available datasets to identify population-based transcriptomic landscape contributing to the aggressiveness of breast cancer in young women |
| title_full | Using publicly available datasets to identify population-based transcriptomic landscape contributing to the aggressiveness of breast cancer in young women |
| title_fullStr | Using publicly available datasets to identify population-based transcriptomic landscape contributing to the aggressiveness of breast cancer in young women |
| title_full_unstemmed | Using publicly available datasets to identify population-based transcriptomic landscape contributing to the aggressiveness of breast cancer in young women |
| title_short | Using publicly available datasets to identify population-based transcriptomic landscape contributing to the aggressiveness of breast cancer in young women |
| title_sort | using publicly available datasets to identify population based transcriptomic landscape contributing to the aggressiveness of breast cancer in young women |
| topic | early-onset breast cancer aggressive metastasis genomics amplification |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.1039037/full |
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