Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media
Culture media is fundamental in clinical bacteriology for the detection and isolation of bacterial pathogens. However, in-house media preparation could be challenging in low-resource settings. InTray<sup>®</sup> cassettes (Biomed Diagnostics) could be a valid alternative as they are comp...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/3/523 |
| _version_ | 1827760166567673856 |
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| author | Alessandra Natale Saoussen Oueslati Alice Rochard Sien Ombelet Daniel Lopez-Baez Liselotte Hardy Jane Cunningham Céline Franquesa Olivier Vandenberg Jean-Baptiste Ronat Thierry Naas |
| author_facet | Alessandra Natale Saoussen Oueslati Alice Rochard Sien Ombelet Daniel Lopez-Baez Liselotte Hardy Jane Cunningham Céline Franquesa Olivier Vandenberg Jean-Baptiste Ronat Thierry Naas |
| author_sort | Alessandra Natale |
| collection | DOAJ |
| description | Culture media is fundamental in clinical bacteriology for the detection and isolation of bacterial pathogens. However, in-house media preparation could be challenging in low-resource settings. InTray<sup>®</sup> cassettes (Biomed Diagnostics) could be a valid alternative as they are compact, ready-to-use media preparations. In this study, we evaluate the use of two InTray media as a subculture alternative for the diagnosis of bloodstream infections: the InTray<sup>®</sup> Müller-Hinton (MH) chocolate and the InTray<sup>®</sup> Colorex™ Screen. The InTray MH chocolate was evaluated in 2 steps: firstly, using simulated positive blood cultures (reference evaluation study), and secondly, using positive blood cultures from a routine clinical laboratory (clinical evaluation study). The Colorex Screen was tested using simulated poly-microbial blood cultures. The sensitivity and specificity of the InTray MH chocolate were respectively 99.2% and 90% in the reference evaluation study and 97.1% and 88.2% in the clinical evaluation study. The time to detection (TTD) was ≤20 h in most positive blood cultures (99.8% and 97% in the two studies, respectively). The InTray<sup>®</sup> MH Chocolate agar showed good performance when used directly from clinical blood cultures for single bacterial infections. However, mixed flora is more challenging to interpret on this media than on Colorex™ Screen, even for an experienced microbiologist. |
| first_indexed | 2024-03-11T09:47:35Z |
| format | Article |
| id | doaj.art-046fb24d19514ab18cb0a83f8476592f |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T09:47:35Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-046fb24d19514ab18cb0a83f8476592f2023-11-16T16:26:00ZengMDPI AGDiagnostics2075-44182023-01-0113352310.3390/diagnostics13030523Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture MediaAlessandra Natale0Saoussen Oueslati1Alice Rochard2Sien Ombelet3Daniel Lopez-Baez4Liselotte Hardy5Jane Cunningham6Céline Franquesa7Olivier Vandenberg8Jean-Baptiste Ronat9Thierry Naas10Médecins Sans Frontières, Operational Center Paris, 75019 Paris, FranceTeam ReSIST, INSERM U1184, Université Paris Saclay, CEA, Inserm, Immunologie des Maladies Virales, Auto-Immunes, Hématologiques et Bactériennes (IMVA-HB/IDMIT), 92265 Fontenay-aux-Roses & Kremlin Bicêtre, FranceMédecins Sans Frontières, Operational Center Paris, 75019 Paris, FranceDepartment of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumHampshire Hospitals Foundation Trust, Basingstoke RG24 9NA, UKDepartment of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumAccess Campaign, Médecins Sans Frontières, 1211 Geneva, SwitzerlandMédecins Sans Frontières, Operational Center Paris, 75019 Paris, FranceCenter for Environmental Health and Occupational Health, School of Public Health, Université Libre de Bruxelles (ULB), 1070 Brussels, BelgiumMédecins Sans Frontières, Operational Center Paris, 75019 Paris, FranceTeam ReSIST, INSERM U1184, Université Paris Saclay, CEA, Inserm, Immunologie des Maladies Virales, Auto-Immunes, Hématologiques et Bactériennes (IMVA-HB/IDMIT), 92265 Fontenay-aux-Roses & Kremlin Bicêtre, FranceCulture media is fundamental in clinical bacteriology for the detection and isolation of bacterial pathogens. However, in-house media preparation could be challenging in low-resource settings. InTray<sup>®</sup> cassettes (Biomed Diagnostics) could be a valid alternative as they are compact, ready-to-use media preparations. In this study, we evaluate the use of two InTray media as a subculture alternative for the diagnosis of bloodstream infections: the InTray<sup>®</sup> Müller-Hinton (MH) chocolate and the InTray<sup>®</sup> Colorex™ Screen. The InTray MH chocolate was evaluated in 2 steps: firstly, using simulated positive blood cultures (reference evaluation study), and secondly, using positive blood cultures from a routine clinical laboratory (clinical evaluation study). The Colorex Screen was tested using simulated poly-microbial blood cultures. The sensitivity and specificity of the InTray MH chocolate were respectively 99.2% and 90% in the reference evaluation study and 97.1% and 88.2% in the clinical evaluation study. The time to detection (TTD) was ≤20 h in most positive blood cultures (99.8% and 97% in the two studies, respectively). The InTray<sup>®</sup> MH Chocolate agar showed good performance when used directly from clinical blood cultures for single bacterial infections. However, mixed flora is more challenging to interpret on this media than on Colorex™ Screen, even for an experienced microbiologist.https://www.mdpi.com/2075-4418/13/3/523InTray cassettesInTray<sup>®</sup> Müller-Hinton (MH) chocolateColorex™ screenbloodstream infectiondiagnosis of sepsisclinical bacteriology in low resource settings |
| spellingShingle | Alessandra Natale Saoussen Oueslati Alice Rochard Sien Ombelet Daniel Lopez-Baez Liselotte Hardy Jane Cunningham Céline Franquesa Olivier Vandenberg Jean-Baptiste Ronat Thierry Naas Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media Diagnostics InTray cassettes InTray<sup>®</sup> Müller-Hinton (MH) chocolate Colorex™ screen bloodstream infection diagnosis of sepsis clinical bacteriology in low resource settings |
| title | Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media |
| title_full | Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media |
| title_fullStr | Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media |
| title_full_unstemmed | Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media |
| title_short | Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media |
| title_sort | evaluation of intray cassettes directly from blood cultures for the diagnosis of sepsis in clinical bacteriology laboratories as an alternative to classic culture media |
| topic | InTray cassettes InTray<sup>®</sup> Müller-Hinton (MH) chocolate Colorex™ screen bloodstream infection diagnosis of sepsis clinical bacteriology in low resource settings |
| url | https://www.mdpi.com/2075-4418/13/3/523 |
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