The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor Cells
Filopodia are multifunctional finger-like plasma membrane protrusions with bundles of actin filaments that exist in virtually all cell types. It has been known for some time that hyaluronan synthesis activity induces filopodial growth. However, because of technical challenges in the studies of these...
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MDPI AG
2020-07-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/7/1908 |
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| author | Heikki Kyykallio Sanna Oikari María Bueno Álvez Carlos José Gallardo Dodd Janne Capra Kirsi Rilla |
| author_facet | Heikki Kyykallio Sanna Oikari María Bueno Álvez Carlos José Gallardo Dodd Janne Capra Kirsi Rilla |
| author_sort | Heikki Kyykallio |
| collection | DOAJ |
| description | Filopodia are multifunctional finger-like plasma membrane protrusions with bundles of actin filaments that exist in virtually all cell types. It has been known for some time that hyaluronan synthesis activity induces filopodial growth. However, because of technical challenges in the studies of these slender and fragile structures, no quantitative analyses have been performed so far to indicate their association with hyaluronan synthesis. In this work we comprehensively address the direct quantification of filopodial traits, covering for the first time length and density measurements in a series of human cancer cell lines with variable levels of hyaluronan synthesis. The synthesis and plasma membrane binding of hyaluronan were manipulated with hyaluronan synthase 3 (HAS3) and hyaluronan receptor CD44 overexpression, and treatments with mannose, 4-methylumbelliferone (4-MU), and glucosamine. The results of this work show that the growth of filopodia was associated with the levels of hyaluronan synthesis but was not dependent on CD44 expression. The results confirm the hypothesis that abundance and length of filopodia in cancer cells is associated with the activity of hyaluronan synthesis. |
| first_indexed | 2024-03-10T18:28:16Z |
| format | Article |
| id | doaj.art-0470c59e25014b2385305d4be46a50c1 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T18:28:16Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-0470c59e25014b2385305d4be46a50c12023-11-20T06:49:40ZengMDPI AGCancers2072-66942020-07-01127190810.3390/cancers12071908The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor CellsHeikki Kyykallio0Sanna Oikari1María Bueno Álvez2Carlos José Gallardo Dodd3Janne Capra4Kirsi Rilla5Institute of Biomedicine, School of Medicine University of Eastern Finland, Yliopistonranta 1E, 70211 Kuopio, FinlandInstitute of Biomedicine, School of Medicine University of Eastern Finland, Yliopistonranta 1E, 70211 Kuopio, FinlandInstitute of Biomedicine, School of Medicine University of Eastern Finland, Yliopistonranta 1E, 70211 Kuopio, FinlandInstitute of Biomedicine, School of Medicine University of Eastern Finland, Yliopistonranta 1E, 70211 Kuopio, FinlandInstitute of Biomedicine, School of Medicine University of Eastern Finland, Yliopistonranta 1E, 70211 Kuopio, FinlandInstitute of Biomedicine, School of Medicine University of Eastern Finland, Yliopistonranta 1E, 70211 Kuopio, FinlandFilopodia are multifunctional finger-like plasma membrane protrusions with bundles of actin filaments that exist in virtually all cell types. It has been known for some time that hyaluronan synthesis activity induces filopodial growth. However, because of technical challenges in the studies of these slender and fragile structures, no quantitative analyses have been performed so far to indicate their association with hyaluronan synthesis. In this work we comprehensively address the direct quantification of filopodial traits, covering for the first time length and density measurements in a series of human cancer cell lines with variable levels of hyaluronan synthesis. The synthesis and plasma membrane binding of hyaluronan were manipulated with hyaluronan synthase 3 (HAS3) and hyaluronan receptor CD44 overexpression, and treatments with mannose, 4-methylumbelliferone (4-MU), and glucosamine. The results of this work show that the growth of filopodia was associated with the levels of hyaluronan synthesis but was not dependent on CD44 expression. The results confirm the hypothesis that abundance and length of filopodia in cancer cells is associated with the activity of hyaluronan synthesis.https://www.mdpi.com/2072-6694/12/7/1908hyaluronanfilopodiaCD44hyaluronan synthasecancer |
| spellingShingle | Heikki Kyykallio Sanna Oikari María Bueno Álvez Carlos José Gallardo Dodd Janne Capra Kirsi Rilla The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor Cells Cancers hyaluronan filopodia CD44 hyaluronan synthase cancer |
| title | The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor Cells |
| title_full | The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor Cells |
| title_fullStr | The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor Cells |
| title_full_unstemmed | The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor Cells |
| title_short | The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor Cells |
| title_sort | density and length of filopodia associate with the activity of hyaluronan synthesis in tumor cells |
| topic | hyaluronan filopodia CD44 hyaluronan synthase cancer |
| url | https://www.mdpi.com/2072-6694/12/7/1908 |
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