Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells
To study the dendritic morphology of retinal ganglion cells in wild-type mice we intracellularly injected these cells with Lucifer yellow in an in vitro preparation of the retina. Subsequently, quantified values of dendritic thickness, number of branching points and level of stratification of 73 Luc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2007-10-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000010 |
| _version_ | 1818119003932983296 |
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| author | L.D. Loopuijt M. da Silva Filho B. Hirt R. Vonthein J. Kremers |
| author_facet | L.D. Loopuijt M. da Silva Filho B. Hirt R. Vonthein J. Kremers |
| author_sort | L.D. Loopuijt |
| collection | DOAJ |
| description | To study the dendritic morphology of retinal ganglion cells in wild-type mice we intracellularly injected these cells with Lucifer yellow in an in vitro preparation of the retina. Subsequently, quantified values of dendritic thickness, number of branching points and level of stratification of 73 Lucifer yellow-filled ganglion cells were analyzed by statistical methods, resulting in a classification into 9 groups. The variables dendritic thickness, number of branching points per cell and level of stratification were independent of each other. Number of branching points and level of stratification were independent of eccentricity, whereas dendritic thickness was positively dependent (r = 0.37) on it. The frequency distribution of dendritic thickness tended to be multimodal, indicating the presence of at least two cell populations composed of neurons with dendritic diameters either smaller or larger than 1.8 µm ("thin" or "thick" dendrites, respectively). Three cells (4.5%) were bistratified, having thick dendrites, and the others (95.5%) were monostratified. Using k-means cluster analysis, monostratified cells with either thin or thick dendrites were further subdivided according to level of stratification and number of branching points: cells with thin dendrites were divided into 2 groups with outer stratification (0-40%) and 2 groups with inner (50-100%) stratification, whereas cells with thick dendrites were divided into one group with outer and 3 groups with inner stratification. We postulate, that one group of cells with thin dendrites resembles cat ß-cells, whereas one group of cells with thick dendrites includes cells that resemble cat a-cells. |
| first_indexed | 2024-12-11T05:03:18Z |
| format | Article |
| id | doaj.art-047261407b97473798c31297ec313889 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-11T05:03:18Z |
| publishDate | 2007-10-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-047261407b97473798c31297ec3138892022-12-22T01:20:06ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2007-10-01401013671382Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cellsL.D. LoopuijtM. da Silva FilhoB. HirtR. VontheinJ. KremersTo study the dendritic morphology of retinal ganglion cells in wild-type mice we intracellularly injected these cells with Lucifer yellow in an in vitro preparation of the retina. Subsequently, quantified values of dendritic thickness, number of branching points and level of stratification of 73 Lucifer yellow-filled ganglion cells were analyzed by statistical methods, resulting in a classification into 9 groups. The variables dendritic thickness, number of branching points per cell and level of stratification were independent of each other. Number of branching points and level of stratification were independent of eccentricity, whereas dendritic thickness was positively dependent (r = 0.37) on it. The frequency distribution of dendritic thickness tended to be multimodal, indicating the presence of at least two cell populations composed of neurons with dendritic diameters either smaller or larger than 1.8 µm ("thin" or "thick" dendrites, respectively). Three cells (4.5%) were bistratified, having thick dendrites, and the others (95.5%) were monostratified. Using k-means cluster analysis, monostratified cells with either thin or thick dendrites were further subdivided according to level of stratification and number of branching points: cells with thin dendrites were divided into 2 groups with outer stratification (0-40%) and 2 groups with inner (50-100%) stratification, whereas cells with thick dendrites were divided into one group with outer and 3 groups with inner stratification. We postulate, that one group of cells with thin dendrites resembles cat ß-cells, whereas one group of cells with thick dendrites includes cells that resemble cat a-cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000010Ganglion cell morphologyRetinal circuitryIntracellular injection of neurotracersLucifer yellowDendritic thickness |
| spellingShingle | L.D. Loopuijt M. da Silva Filho B. Hirt R. Vonthein J. Kremers Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells Brazilian Journal of Medical and Biological Research Ganglion cell morphology Retinal circuitry Intracellular injection of neurotracers Lucifer yellow Dendritic thickness |
| title | Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells |
| title_full | Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells |
| title_fullStr | Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells |
| title_full_unstemmed | Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells |
| title_short | Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells |
| title_sort | dendritic thickness a morphometric parameter to classify mouse retinal ganglion cells |
| topic | Ganglion cell morphology Retinal circuitry Intracellular injection of neurotracers Lucifer yellow Dendritic thickness |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000010 |
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