Sepiapterin reductase (Spra and Sprb) regulate carotenoid and pteridine metabolism influencing the koi carp (Cyprinus carpio L.) coloration
The evolution of teleost coloration is strongly linked with the fish-specific genome duplication (FSGD). Sepiapterin reductase (Spr) is commonly required in pteridine pigment synthesis and two paralogs are found in teleosts. However, the functions of spra and sprb in pigmentation have not been compl...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423004398 |
| _version_ | 1827143686476005376 |
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| author | X. Tian Y.S. Shan N.N. Peng X. Ma X. Shi H. Li X.J. Li X.L. Meng |
| author_facet | X. Tian Y.S. Shan N.N. Peng X. Ma X. Shi H. Li X.J. Li X.L. Meng |
| author_sort | X. Tian |
| collection | DOAJ |
| description | The evolution of teleost coloration is strongly linked with the fish-specific genome duplication (FSGD). Sepiapterin reductase (Spr) is commonly required in pteridine pigment synthesis and two paralogs are found in teleosts. However, the functions of spra and sprb in pigmentation have not been completely elucidated. Here we found that spra was intensively expressed in skin, fins and scales of koi carp, while sprb was ubiquitously expressed in all tissues. Then, the Spr enzyme activity inhibitor, sulfapyridine (SFD), was used to explore the phenotype and genes variation after spr inhibition. Following the administration of SFD, spra was significantly suppressed but sprb was up-regulated in skin. Additionally, the color phenotypes shifted from red to yellow due to a reduction in carotenoid concentration and the number of xanthophores/erythrophores in scales. Furthermore, the genes involved in carotenoid metabolism were influenced. However, pteridine metabolism-related genes rarely exhibited obvious changes. The results indicate that spra inhibition resulted in the dysregulation of carotenoid metabolism and pigment cells reduction, whereas sprb was likely to maintain the conserved functions in pteridine synthesis for metabolic homoeostasis. This study provided evidences for the different functions of spr paralogs in carotenoid and pteridine metabolism and for better understanding of the relationship between genome duplication and color pattern diversification. |
| first_indexed | 2024-03-08T19:01:26Z |
| format | Article |
| id | doaj.art-047348dafbf141b4a888d9dd934425fa |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2025-03-20T19:46:46Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-047348dafbf141b4a888d9dd934425fa2024-08-21T06:30:35ZengElsevierAquaculture Reports2352-51342024-02-0134101900Sepiapterin reductase (Spra and Sprb) regulate carotenoid and pteridine metabolism influencing the koi carp (Cyprinus carpio L.) colorationX. Tian0Y.S. Shan1N.N. Peng2X. Ma3X. Shi4H. Li5X.J. Li6X.L. Meng7College of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaHenan Academy of Fishery Sciences, Zhengzhou 450040, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR China; Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, PR China; Corresponding author at: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.The evolution of teleost coloration is strongly linked with the fish-specific genome duplication (FSGD). Sepiapterin reductase (Spr) is commonly required in pteridine pigment synthesis and two paralogs are found in teleosts. However, the functions of spra and sprb in pigmentation have not been completely elucidated. Here we found that spra was intensively expressed in skin, fins and scales of koi carp, while sprb was ubiquitously expressed in all tissues. Then, the Spr enzyme activity inhibitor, sulfapyridine (SFD), was used to explore the phenotype and genes variation after spr inhibition. Following the administration of SFD, spra was significantly suppressed but sprb was up-regulated in skin. Additionally, the color phenotypes shifted from red to yellow due to a reduction in carotenoid concentration and the number of xanthophores/erythrophores in scales. Furthermore, the genes involved in carotenoid metabolism were influenced. However, pteridine metabolism-related genes rarely exhibited obvious changes. The results indicate that spra inhibition resulted in the dysregulation of carotenoid metabolism and pigment cells reduction, whereas sprb was likely to maintain the conserved functions in pteridine synthesis for metabolic homoeostasis. This study provided evidences for the different functions of spr paralogs in carotenoid and pteridine metabolism and for better understanding of the relationship between genome duplication and color pattern diversification.http://www.sciencedirect.com/science/article/pii/S2352513423004398spra/bParalogs functionCarotenoid metabolismPteridine metabolismPigmentation |
| spellingShingle | X. Tian Y.S. Shan N.N. Peng X. Ma X. Shi H. Li X.J. Li X.L. Meng Sepiapterin reductase (Spra and Sprb) regulate carotenoid and pteridine metabolism influencing the koi carp (Cyprinus carpio L.) coloration Aquaculture Reports spra/b Paralogs function Carotenoid metabolism Pteridine metabolism Pigmentation |
| title | Sepiapterin reductase (Spra and Sprb) regulate carotenoid and pteridine metabolism influencing the koi carp (Cyprinus carpio L.) coloration |
| title_full | Sepiapterin reductase (Spra and Sprb) regulate carotenoid and pteridine metabolism influencing the koi carp (Cyprinus carpio L.) coloration |
| title_fullStr | Sepiapterin reductase (Spra and Sprb) regulate carotenoid and pteridine metabolism influencing the koi carp (Cyprinus carpio L.) coloration |
| title_full_unstemmed | Sepiapterin reductase (Spra and Sprb) regulate carotenoid and pteridine metabolism influencing the koi carp (Cyprinus carpio L.) coloration |
| title_short | Sepiapterin reductase (Spra and Sprb) regulate carotenoid and pteridine metabolism influencing the koi carp (Cyprinus carpio L.) coloration |
| title_sort | sepiapterin reductase spra and sprb regulate carotenoid and pteridine metabolism influencing the koi carp cyprinus carpio l coloration |
| topic | spra/b Paralogs function Carotenoid metabolism Pteridine metabolism Pigmentation |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423004398 |
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