Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)
Feline herpesvirus type 1 (FHV-1) is endemic in captive cheetahs and sporadically causes devastating disease. Modified live vaccines (MLV), intended for use in domestic cats, are used in some captive cheetah populations and have been anecdotally linked to disease in certain subpopulations. Ten FHV-1...
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MDPI AG
2021-11-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/11/2307 |
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| author | Morgan E. Marino Melanie A. Mironovich Nikole E. Ineck Scott B. Citino Jessica A. Emerson David J. Maggs Lyndon M. Coghill Edward J. Dubovi Rachel C. Turner Renee T. Carter Andrew C. Lewin |
| author_facet | Morgan E. Marino Melanie A. Mironovich Nikole E. Ineck Scott B. Citino Jessica A. Emerson David J. Maggs Lyndon M. Coghill Edward J. Dubovi Rachel C. Turner Renee T. Carter Andrew C. Lewin |
| author_sort | Morgan E. Marino |
| collection | DOAJ |
| description | Feline herpesvirus type 1 (FHV-1) is endemic in captive cheetahs and sporadically causes devastating disease. Modified live vaccines (MLV), intended for use in domestic cats, are used in some captive cheetah populations and have been anecdotally linked to disease in certain subpopulations. Ten FHV-1 isolates from ten captive cheetahs and one isolate from an MLV used to inoculate four of the host animals were analyzed. Viral DNA was extracted for full-genome sequencing by Illumina MiSeq with viral genomes then used for phylogenomic and recombinational analyses. The FHV-1 shed by vaccinated cheetahs were almost identical to the MLV, with few variants among viral genomes. Eight cheetah FHV-1 isolates and the MLV were grouped in a clade along with FHV-1 isolates from domestic cats in the USA. The remaining two cheetah FHV-1 isolates (unknown host vaccine status) were not associated with a clade. The likely ancestral origin of these two isolates involves recombination events between Australian domestic cat and cheetah FHV-1 isolates. Collectively, these data suggest that the MLV is capable of causing clinical disease and viral shedding in some cheetahs and represents evidence of interspecies transmission of virus between domestic and wild cats. |
| first_indexed | 2024-03-10T04:58:46Z |
| format | Article |
| id | doaj.art-0477551e34924dbcbf0ea52b409f2c3d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T04:58:46Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-0477551e34924dbcbf0ea52b409f2c3d2023-11-23T01:58:32ZengMDPI AGViruses1999-49152021-11-011311230710.3390/v13112307Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)Morgan E. Marino0Melanie A. Mironovich1Nikole E. Ineck2Scott B. Citino3Jessica A. Emerson4David J. Maggs5Lyndon M. Coghill6Edward J. Dubovi7Rachel C. Turner8Renee T. Carter9Andrew C. Lewin10Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USADepartment of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USADepartment of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USAWhite Oak Conservation, Yulee, FL 32097, USAWhite Oak Conservation, Yulee, FL 32097, USADepartment of Surgical and Radiological Sciences, University of California Davis School of Veterinary Medicine, Davis, CA 95616, USABioinformatics and Analytics Core, University of Missouri, Columbia, MO 65211, USADepartment of Population Medicine and Diagnostic Sciences, Cornell University College of Veterinary Sciences, Ithaca, NY 14853, USABirmingham Zoo, 2630 Cahaba Road, Birmingham, AL 35223, USADepartment of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USADepartment of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USAFeline herpesvirus type 1 (FHV-1) is endemic in captive cheetahs and sporadically causes devastating disease. Modified live vaccines (MLV), intended for use in domestic cats, are used in some captive cheetah populations and have been anecdotally linked to disease in certain subpopulations. Ten FHV-1 isolates from ten captive cheetahs and one isolate from an MLV used to inoculate four of the host animals were analyzed. Viral DNA was extracted for full-genome sequencing by Illumina MiSeq with viral genomes then used for phylogenomic and recombinational analyses. The FHV-1 shed by vaccinated cheetahs were almost identical to the MLV, with few variants among viral genomes. Eight cheetah FHV-1 isolates and the MLV were grouped in a clade along with FHV-1 isolates from domestic cats in the USA. The remaining two cheetah FHV-1 isolates (unknown host vaccine status) were not associated with a clade. The likely ancestral origin of these two isolates involves recombination events between Australian domestic cat and cheetah FHV-1 isolates. Collectively, these data suggest that the MLV is capable of causing clinical disease and viral shedding in some cheetahs and represents evidence of interspecies transmission of virus between domestic and wild cats.https://www.mdpi.com/1999-4915/13/11/2307feline herpesvirusherpesVaricelloviruscheetahgenome sequencingphylogenomic |
| spellingShingle | Morgan E. Marino Melanie A. Mironovich Nikole E. Ineck Scott B. Citino Jessica A. Emerson David J. Maggs Lyndon M. Coghill Edward J. Dubovi Rachel C. Turner Renee T. Carter Andrew C. Lewin Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>) Viruses feline herpesvirus herpes Varicellovirus cheetah genome sequencing phylogenomic |
| title | Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>) |
| title_full | Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>) |
| title_fullStr | Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>) |
| title_full_unstemmed | Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>) |
| title_short | Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>) |
| title_sort | full viral genome sequencing and phylogenomic analysis of feline herpesvirus type 1 fhv 1 in cheetahs i acinonyx jubatus i |
| topic | feline herpesvirus herpes Varicellovirus cheetah genome sequencing phylogenomic |
| url | https://www.mdpi.com/1999-4915/13/11/2307 |
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