Bioassay-Guided Fractionation of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory Study
Bioprospecting native Australian plants offers the potential discovery of latent and novel bioactive compounds. The promising cytotoxic and antibacterial activity of methanolic extracts of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> led to further fra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/6/807 |
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| author | Janice Mani Joel Johnson Holly Hosking Luke Schmidt Ryan Batley Ryan du Preez Daniel Broszczak Kerry Walsh Paul Neilsen Mani Naiker |
| author_facet | Janice Mani Joel Johnson Holly Hosking Luke Schmidt Ryan Batley Ryan du Preez Daniel Broszczak Kerry Walsh Paul Neilsen Mani Naiker |
| author_sort | Janice Mani |
| collection | DOAJ |
| description | Bioprospecting native Australian plants offers the potential discovery of latent and novel bioactive compounds. The promising cytotoxic and antibacterial activity of methanolic extracts of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> led to further fractionation and isolation using our laboratory’s bioassay-guided fractionation protocol. Hence, the aim of this study was to further evaluate the bioactivity of the fractions and subfractions and characterize bioactive compounds using liquid chromatography mass spectroscopy (LC-MS/MS) and gas chromatography MS (GC-MS). Compounds tentatively identified in <i>P. angustifolium</i> Fraction 1 using LC-ESI-QTOF-MS/MS were chlorogenic acid and/or neochlorogenic acid, bergapten, berberine, 8′-epitanegool and rosmarinic acid. GC-MS analysis data showed the presence of around 100 compounds, mainly comprising carboxylic acids, sugars, sugar alcohols, amino acids and monoalkylglycerols. Furthermore, the fractions obtained from <i>T. ferdinandiana</i> flesh extracts showed no cytotoxicity, except against HT29 cell lines, and only Fraction 2 exhibited some antibacterial activity. The reduced bioactivity observed in the <i>T. ferdinandiana</i> fractions could be attributed to the potential loss of synergy as compounds become separated within the fractions. As a result, the further fractionation and separation of compounds in these samples was not pursued. However, additional dose-dependent studies are warranted to validate the bioactivity of <i>T. ferdinandiana</i> flesh fractions, particularly since this is an understudied species. Moreover, LC-MS/GC-MS studies confirm the presence of bioactive compounds in <i>P. angustifolium</i> Fraction 1/subfractions, which helps to explain the significant acute anticancer activity of this plant. The screening process designed in this study has the potential to pave the way for developing scientifically validated phytochemical/bioactivity information on ethnomedicinal plants, thereby facilitating further bioprospecting efforts and supporting the discovery of novel drugs in modern medicine. |
| first_indexed | 2024-04-24T17:53:28Z |
| format | Article |
| id | doaj.art-0477b1272e984a5a9003ebf0bfbe4ca4 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T17:53:28Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-0477b1272e984a5a9003ebf0bfbe4ca42024-03-27T14:00:42ZengMDPI AGPlants2223-77472024-03-0113680710.3390/plants13060807Bioassay-Guided Fractionation of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory StudyJanice Mani0Joel Johnson1Holly Hosking2Luke Schmidt3Ryan Batley4Ryan du Preez5Daniel Broszczak6Kerry Walsh7Paul Neilsen8Mani Naiker9College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, AustraliaCollege of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, AustraliaCollege of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, AustraliaSchool of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD 4000, AustraliaCollege of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, AustraliaCollege of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, AustraliaSchool of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD 4000, AustraliaCollege of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, AustraliaCollege of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, AustraliaCollege of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, AustraliaBioprospecting native Australian plants offers the potential discovery of latent and novel bioactive compounds. The promising cytotoxic and antibacterial activity of methanolic extracts of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> led to further fractionation and isolation using our laboratory’s bioassay-guided fractionation protocol. Hence, the aim of this study was to further evaluate the bioactivity of the fractions and subfractions and characterize bioactive compounds using liquid chromatography mass spectroscopy (LC-MS/MS) and gas chromatography MS (GC-MS). Compounds tentatively identified in <i>P. angustifolium</i> Fraction 1 using LC-ESI-QTOF-MS/MS were chlorogenic acid and/or neochlorogenic acid, bergapten, berberine, 8′-epitanegool and rosmarinic acid. GC-MS analysis data showed the presence of around 100 compounds, mainly comprising carboxylic acids, sugars, sugar alcohols, amino acids and monoalkylglycerols. Furthermore, the fractions obtained from <i>T. ferdinandiana</i> flesh extracts showed no cytotoxicity, except against HT29 cell lines, and only Fraction 2 exhibited some antibacterial activity. The reduced bioactivity observed in the <i>T. ferdinandiana</i> fractions could be attributed to the potential loss of synergy as compounds become separated within the fractions. As a result, the further fractionation and separation of compounds in these samples was not pursued. However, additional dose-dependent studies are warranted to validate the bioactivity of <i>T. ferdinandiana</i> flesh fractions, particularly since this is an understudied species. Moreover, LC-MS/GC-MS studies confirm the presence of bioactive compounds in <i>P. angustifolium</i> Fraction 1/subfractions, which helps to explain the significant acute anticancer activity of this plant. The screening process designed in this study has the potential to pave the way for developing scientifically validated phytochemical/bioactivity information on ethnomedicinal plants, thereby facilitating further bioprospecting efforts and supporting the discovery of novel drugs in modern medicine.https://www.mdpi.com/2223-7747/13/6/807bioassay-guided fractionation<i>Pittosporum angustifolium</i>bioactive compoundsphytochemicalsphenolic compoundsantioxidants |
| spellingShingle | Janice Mani Joel Johnson Holly Hosking Luke Schmidt Ryan Batley Ryan du Preez Daniel Broszczak Kerry Walsh Paul Neilsen Mani Naiker Bioassay-Guided Fractionation of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory Study Plants bioassay-guided fractionation <i>Pittosporum angustifolium</i> bioactive compounds phytochemicals phenolic compounds antioxidants |
| title | Bioassay-Guided Fractionation of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory Study |
| title_full | Bioassay-Guided Fractionation of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory Study |
| title_fullStr | Bioassay-Guided Fractionation of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory Study |
| title_full_unstemmed | Bioassay-Guided Fractionation of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory Study |
| title_short | Bioassay-Guided Fractionation of <i>Pittosporum angustifolium</i> and <i>Terminalia ferdinandiana</i> with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory Study |
| title_sort | bioassay guided fractionation of i pittosporum angustifolium i and i terminalia ferdinandiana i with liquid chromatography mass spectroscopy and gas chromatography mass spectroscopy exploratory study |
| topic | bioassay-guided fractionation <i>Pittosporum angustifolium</i> bioactive compounds phytochemicals phenolic compounds antioxidants |
| url | https://www.mdpi.com/2223-7747/13/6/807 |
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