Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered <it>Pseudomonas putida</it> KT2440
<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas putida</it> KT2440 is able to synthesize large amounts of medium-chain-length polyhydroxyalkanoates (mcl-PHAs). To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, x...
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| Format: | Article |
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BMC
2012-08-01
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| Series: | BMC Biotechnology |
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| Online Access: | http://www.biomedcentral.com/1472-6750/12/53 |
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| author | Le Meur Sylvaine Zinn Manfred Egli Thomas Thöny-Meyer Linda Ren Qun |
| author_facet | Le Meur Sylvaine Zinn Manfred Egli Thomas Thöny-Meyer Linda Ren Qun |
| author_sort | Le Meur Sylvaine |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Pseudomonas putida</it> KT2440 is able to synthesize large amounts of medium-chain-length polyhydroxyalkanoates (mcl-PHAs). To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, xylose, a hemicellulose derivate, was tested as the growth carbon source in an engineered <it>P. putida</it> KT2440 strain.</p> <p>Results</p> <p>The genes encoding xylose isomerase (XylA) and xylulokinase (XylB) from <it>Escherichia coli</it> W3110 were introduced into <it>P. putida</it> KT2440. The recombinant KT2440 exhibited a XylA activity of 1.47 U and a XylB activity of 0.97 U when grown on a defined medium supplemented with xylose. The cells reached a maximum specific growth rate of 0.24 h<sup>-1</sup> and a final cell dry weight (CDW) of 2.5 g L<sup>-1</sup> with a maximal yield of 0.5 g CDW g<sup>-1</sup> xylose. Since no mcl-PHA was accumulated from xylose, mcl-PHA production can be controlled by the addition of fatty acids leading to tailor-made PHA compositions. Sequential feeding strategy was applied using xylose as the growth substrate and octanoic acid as the precursor for mcl-PHA production. In this way, up to 20% w w<sup>-1</sup> of mcl-PHA was obtained. A yield of 0.37 g mcl-PHA per g octanoic acid was achieved under the employed conditions.</p> <p>Conclusions</p> <p>Sequential feeding of relatively cheap carbohydrates and expensive fatty acids is a practical way to achieve more cost-effective mcl-PHA production. This study is the first reported attempt to produce mcl-PHA by using xylose as the growth substrate. Further process optimizations to achieve higher cell density and higher productivity of mcl-PHA should be investigated. These scientific exercises will undoubtedly contribute to the economic feasibility of mcl-PHA production from renewable feedstock.</p> |
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| spelling | doaj.art-0478b684d089424cbcbffcd4cea98d6d2022-12-22T01:07:41ZengBMCBMC Biotechnology1472-67502012-08-011215310.1186/1472-6750-12-53Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered <it>Pseudomonas putida</it> KT2440Le Meur SylvaineZinn ManfredEgli ThomasThöny-Meyer LindaRen Qun<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas putida</it> KT2440 is able to synthesize large amounts of medium-chain-length polyhydroxyalkanoates (mcl-PHAs). To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, xylose, a hemicellulose derivate, was tested as the growth carbon source in an engineered <it>P. putida</it> KT2440 strain.</p> <p>Results</p> <p>The genes encoding xylose isomerase (XylA) and xylulokinase (XylB) from <it>Escherichia coli</it> W3110 were introduced into <it>P. putida</it> KT2440. The recombinant KT2440 exhibited a XylA activity of 1.47 U and a XylB activity of 0.97 U when grown on a defined medium supplemented with xylose. The cells reached a maximum specific growth rate of 0.24 h<sup>-1</sup> and a final cell dry weight (CDW) of 2.5 g L<sup>-1</sup> with a maximal yield of 0.5 g CDW g<sup>-1</sup> xylose. Since no mcl-PHA was accumulated from xylose, mcl-PHA production can be controlled by the addition of fatty acids leading to tailor-made PHA compositions. Sequential feeding strategy was applied using xylose as the growth substrate and octanoic acid as the precursor for mcl-PHA production. In this way, up to 20% w w<sup>-1</sup> of mcl-PHA was obtained. A yield of 0.37 g mcl-PHA per g octanoic acid was achieved under the employed conditions.</p> <p>Conclusions</p> <p>Sequential feeding of relatively cheap carbohydrates and expensive fatty acids is a practical way to achieve more cost-effective mcl-PHA production. This study is the first reported attempt to produce mcl-PHA by using xylose as the growth substrate. Further process optimizations to achieve higher cell density and higher productivity of mcl-PHA should be investigated. These scientific exercises will undoubtedly contribute to the economic feasibility of mcl-PHA production from renewable feedstock.</p>http://www.biomedcentral.com/1472-6750/12/53mcl-PHAXyloseOctanoic acid<it>Pseudomonas putida</it> KT2440Sequential-feedingTailor-made PHA |
| spellingShingle | Le Meur Sylvaine Zinn Manfred Egli Thomas Thöny-Meyer Linda Ren Qun Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered <it>Pseudomonas putida</it> KT2440 BMC Biotechnology mcl-PHA Xylose Octanoic acid <it>Pseudomonas putida</it> KT2440 Sequential-feeding Tailor-made PHA |
| title | Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered <it>Pseudomonas putida</it> KT2440 |
| title_full | Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered <it>Pseudomonas putida</it> KT2440 |
| title_fullStr | Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered <it>Pseudomonas putida</it> KT2440 |
| title_full_unstemmed | Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered <it>Pseudomonas putida</it> KT2440 |
| title_short | Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered <it>Pseudomonas putida</it> KT2440 |
| title_sort | production of medium chain length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered it pseudomonas putida it kt2440 |
| topic | mcl-PHA Xylose Octanoic acid <it>Pseudomonas putida</it> KT2440 Sequential-feeding Tailor-made PHA |
| url | http://www.biomedcentral.com/1472-6750/12/53 |
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