Single Cell Isolation and Analysis
Increasing evidence shows that the heterogeneity of individual cells within a genetically identical population can be critical to their peculiar function and fate. Conventional cell based assays mainly analysis the average responses from a population cells, while the difference within individual cel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00116/full |
| _version_ | 1830449219621617664 |
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| author | Ping Hu wenhua Zhang Hongbo Xin Glenn Deng |
| author_facet | Ping Hu wenhua Zhang Hongbo Xin Glenn Deng |
| author_sort | Ping Hu |
| collection | DOAJ |
| description | Increasing evidence shows that the heterogeneity of individual cells within a genetically identical population can be critical to their peculiar function and fate. Conventional cell based assays mainly analysis the average responses from a population cells, while the difference within individual cells may often be masked. The cell size, RNA transcripts and protein expression level are quite different within individual cells and these variations are key point to answer the problems in cancer, neurobiology, stem cell biology, immunology and developmental biology. To better understand the cell-to-cell variations, the single cell analysis can provide much more detailed information which may be helpful for therapeutic decisions in an increasingly personalized medicine. In this review, we will focus on the recent development in single cell analysis, including methods used in single cell isolation, analysis and some application examples. The review provides the historical background to single cell analysis, discusses limitations, and current and future possibilities in this exciting field of research. |
| first_indexed | 2024-12-21T07:50:32Z |
| format | Article |
| id | doaj.art-04794f0d0e164ee4a044bdddddb6daf0 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-21T07:50:32Z |
| publishDate | 2016-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-04794f0d0e164ee4a044bdddddb6daf02022-12-21T19:11:04ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2016-10-01410.3389/fcell.2016.00116212316Single Cell Isolation and AnalysisPing Hu0wenhua Zhang1Hongbo Xin2Glenn Deng3Nanchang UniversityNanchang UniversityNanchang UniveristyStanford UniversityIncreasing evidence shows that the heterogeneity of individual cells within a genetically identical population can be critical to their peculiar function and fate. Conventional cell based assays mainly analysis the average responses from a population cells, while the difference within individual cells may often be masked. The cell size, RNA transcripts and protein expression level are quite different within individual cells and these variations are key point to answer the problems in cancer, neurobiology, stem cell biology, immunology and developmental biology. To better understand the cell-to-cell variations, the single cell analysis can provide much more detailed information which may be helpful for therapeutic decisions in an increasingly personalized medicine. In this review, we will focus on the recent development in single cell analysis, including methods used in single cell isolation, analysis and some application examples. The review provides the historical background to single cell analysis, discusses limitations, and current and future possibilities in this exciting field of research.http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00116/fullSequencing.single cellIsolationheterogeneity |
| spellingShingle | Ping Hu wenhua Zhang Hongbo Xin Glenn Deng Single Cell Isolation and Analysis Frontiers in Cell and Developmental Biology Sequencing . single cell Isolation heterogeneity |
| title | Single Cell Isolation and Analysis |
| title_full | Single Cell Isolation and Analysis |
| title_fullStr | Single Cell Isolation and Analysis |
| title_full_unstemmed | Single Cell Isolation and Analysis |
| title_short | Single Cell Isolation and Analysis |
| title_sort | single cell isolation and analysis |
| topic | Sequencing . single cell Isolation heterogeneity |
| url | http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00116/full |
| work_keys_str_mv | AT pinghu singlecellisolationandanalysis AT wenhuazhang singlecellisolationandanalysis AT hongboxin singlecellisolationandanalysis AT glenndeng singlecellisolationandanalysis |