Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity
Mitochondrial gene mutation plays a role in the development of type two diabetes mellitus (T2DM). A point mutation in the mitochondrial gene Nicotinamide adenine dinucleotide dehydrogenase 1 (mtDNA ND1) gene mainly reported as the most common mutation related to T2DM. However, several studies have i...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2014-12-01
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| Series: | Hayati Journal of Biosciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916300900 |
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| author | AMIEN RAMADHAN ISHAK RINI PUSPITANINGRUM RISMA DWI UTARI MELLA FERANIA CHRIS ADHIYANTO TAKENORI NITTA AB SUSANTO HATTORI YUKIO YASUHIRO YAMASHIRO |
| author_facet | AMIEN RAMADHAN ISHAK RINI PUSPITANINGRUM RISMA DWI UTARI MELLA FERANIA CHRIS ADHIYANTO TAKENORI NITTA AB SUSANTO HATTORI YUKIO YASUHIRO YAMASHIRO |
| author_sort | AMIEN RAMADHAN ISHAK |
| collection | DOAJ |
| description | Mitochondrial gene mutation plays a role in the development of type two diabetes mellitus (T2DM). A point mutation in the mitochondrial gene Nicotinamide adenine dinucleotide dehydrogenase 1 (mtDNA ND1) gene mainly reported as the most common mutation related to T2DM. However, several studies have identified another SNP (single-nucleotide polymorphisms) in the RNA region of mtDNA from patients from specific ethnic populations in Indonesia. Building on those findings, this study aimed to use PCR and DNA sequencing technology to identify nucleotides in RNA and ND1 fragment from 20 Gorontalonese and 20 Javanese T2DM patients, that may trigger T2DM expression. The results showed successful amplification of RNA along 294 bp for all samples. From these samples, we found two types of point mutation in Javanese patients in the G3316A and T3200C points of the rRNA and ND1 gene. In samples taken from Gorontalonese patients, no mutation were found in the RNA or ND1 region. We conclude that T2DM was triggered differently in our two populations. While genetic mutation is implicated for the 20 Javanese patients, T2DM pathogenesis in the Gorontalonese patients must be traced to other genetic, environmental, or behavioral factors. |
| first_indexed | 2024-12-11T22:38:17Z |
| format | Article |
| id | doaj.art-0479a323179e4a9ea6e9932868ec907b |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-11T22:38:17Z |
| publishDate | 2014-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-0479a323179e4a9ea6e9932868ec907b2022-12-22T00:47:52ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192014-12-0121415916510.4308/hjb.21.4.159Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese EthnicityAMIEN RAMADHAN ISHAK0RINI PUSPITANINGRUM1RISMA DWI UTARI2MELLA FERANIA3CHRIS ADHIYANTO4TAKENORI NITTA5AB SUSANTO6HATTORI YUKIO7YASUHIRO YAMASHIRO8Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jalan Pemuda 10 Rawamangun, Jakarta 13220, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jalan Pemuda 10 Rawamangun, Jakarta 13220, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jalan Pemuda 10 Rawamangun, Jakarta 13220, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jalan Pemuda 10 Rawamangun, Jakarta 13220, IndonesiaFaculty of Medicines and Health Science, Universitas Negeri Islam Syarif Hidayatullah Jakarta, Jalan Ir. H. Juanda No.95, Ciputat 15412, IndonesiaFaculty of Health Sciences, Yamaguchi University School of Medicine 1.1.1 Minami Kogushi Ube. Yamaguchi, JapanFaculty of Fisheries and Marine Sciences, Kampus Tembalang - University Diponegoro, Semarang 50276, IndonesiaFaculty of Health Sciences, Yamaguchi University School of Medicine 1.1.1 Minami Kogushi Ube. Yamaguchi, JapanFaculty of Health Sciences, Yamaguchi University School of Medicine 1.1.1 Minami Kogushi Ube. Yamaguchi, JapanMitochondrial gene mutation plays a role in the development of type two diabetes mellitus (T2DM). A point mutation in the mitochondrial gene Nicotinamide adenine dinucleotide dehydrogenase 1 (mtDNA ND1) gene mainly reported as the most common mutation related to T2DM. However, several studies have identified another SNP (single-nucleotide polymorphisms) in the RNA region of mtDNA from patients from specific ethnic populations in Indonesia. Building on those findings, this study aimed to use PCR and DNA sequencing technology to identify nucleotides in RNA and ND1 fragment from 20 Gorontalonese and 20 Javanese T2DM patients, that may trigger T2DM expression. The results showed successful amplification of RNA along 294 bp for all samples. From these samples, we found two types of point mutation in Javanese patients in the G3316A and T3200C points of the rRNA and ND1 gene. In samples taken from Gorontalonese patients, no mutation were found in the RNA or ND1 region. We conclude that T2DM was triggered differently in our two populations. While genetic mutation is implicated for the 20 Javanese patients, T2DM pathogenesis in the Gorontalonese patients must be traced to other genetic, environmental, or behavioral factors.http://www.sciencedirect.com/science/article/pii/S1978301916300900mtDNA ND1 fragment genetype 2 diabetes mellitusJavaneseGorontalonese |
| spellingShingle | AMIEN RAMADHAN ISHAK RINI PUSPITANINGRUM RISMA DWI UTARI MELLA FERANIA CHRIS ADHIYANTO TAKENORI NITTA AB SUSANTO HATTORI YUKIO YASUHIRO YAMASHIRO Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity Hayati Journal of Biosciences mtDNA ND1 fragment gene type 2 diabetes mellitus Javanese Gorontalonese |
| title | Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity |
| title_full | Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity |
| title_fullStr | Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity |
| title_full_unstemmed | Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity |
| title_short | Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity |
| title_sort | mutation of mtdna nd1 gene in 20 type 2 diabetes mellitus patients of gorontalonese and javanese ethnicity |
| topic | mtDNA ND1 fragment gene type 2 diabetes mellitus Javanese Gorontalonese |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916300900 |
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