Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds
In this paper, we present the morphological characteristics of the seed and micromorphological, anatomical and chemical characteristics of the seed coat of pea (Pisum sativum L.) genotypes, Jezero, Javor and NS Junior. Our aim was to investigate whether these genotypes can be differentiated...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600126L.pdf |
| Summary: | In this paper, we present the morphological characteristics of the seed and
micromorphological, anatomical and chemical characteristics of the seed coat
of pea (Pisum sativum L.) genotypes, Jezero, Javor and NS Junior. Our aim was
to investigate whether these genotypes can be differentiated based on seed
coat morphoanatomical characteristics, depending on the harvest treatment.
The observations and measurements of seed coat cross-sections were performed
using light microscopy. The seed coat surface was observed using SEM. A
tuberculate seed coat surface characterized all examined pea genotypes, and
the average diameter of the tubercle was about 12 μm. Statistical and
laboratory analyses revealed that major damage was the most frequent defect
type as the result of mechanized harvest in all the examined genotypes.
Genotype NS Junior had the shortest seed length (6.1 mm). Micromorphological
analysis revealed that the seed surface was tuberculate in all genotypes. The
genotype Jezero had the highest number of tubercle ribs (11.0) and a
significantly higher proportion of parenchyma tissue (50.6%), while NS Junior
was characterized by the greatest share of macrosclereids (49.8%). The
highest number of osteosclereids (832/mm2) was counted in genotype Javor. In
addition, genotype NS Junior stands out due to the highest percentage of
crude fiber (62.75 g/100g) in the seed coat. There was a marked difference
among the studied genotypes with regard to the seed coat morphoanatomical
characteristics, which is confirmed by the results of multivariate
discriminant analysis (MDA). These results suggested that the morphological,
micromorphological and anatomical characteristics of the seed might have an
impact on the seed coat damage level at harvest. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 31024 and Grant no. 173002] |
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| ISSN: | 0354-4664 1821-4339 |