Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds
In this paper, we present the morphological characteristics of the seed and micromorphological, anatomical and chemical characteristics of the seed coat of pea (Pisum sativum L.) genotypes, Jezero, Javor and NS Junior. Our aim was to investigate whether these genotypes can be differentiated...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600126L.pdf |
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| author | Lazarević Jelena Zorić Lana Karagić Đura Milošević Branko Karanović Dunja Milić Dubravka Tepić Aleksandra Luković Jadranka |
| author_facet | Lazarević Jelena Zorić Lana Karagić Đura Milošević Branko Karanović Dunja Milić Dubravka Tepić Aleksandra Luković Jadranka |
| author_sort | Lazarević Jelena |
| collection | DOAJ |
| description | In this paper, we present the morphological characteristics of the seed and
micromorphological, anatomical and chemical characteristics of the seed coat
of pea (Pisum sativum L.) genotypes, Jezero, Javor and NS Junior. Our aim was
to investigate whether these genotypes can be differentiated based on seed
coat morphoanatomical characteristics, depending on the harvest treatment.
The observations and measurements of seed coat cross-sections were performed
using light microscopy. The seed coat surface was observed using SEM. A
tuberculate seed coat surface characterized all examined pea genotypes, and
the average diameter of the tubercle was about 12 μm. Statistical and
laboratory analyses revealed that major damage was the most frequent defect
type as the result of mechanized harvest in all the examined genotypes.
Genotype NS Junior had the shortest seed length (6.1 mm). Micromorphological
analysis revealed that the seed surface was tuberculate in all genotypes. The
genotype Jezero had the highest number of tubercle ribs (11.0) and a
significantly higher proportion of parenchyma tissue (50.6%), while NS Junior
was characterized by the greatest share of macrosclereids (49.8%). The
highest number of osteosclereids (832/mm2) was counted in genotype Javor. In
addition, genotype NS Junior stands out due to the highest percentage of
crude fiber (62.75 g/100g) in the seed coat. There was a marked difference
among the studied genotypes with regard to the seed coat morphoanatomical
characteristics, which is confirmed by the results of multivariate
discriminant analysis (MDA). These results suggested that the morphological,
micromorphological and anatomical characteristics of the seed might have an
impact on the seed coat damage level at harvest. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 31024 and Grant no. 173002] |
| first_indexed | 2024-12-22T05:45:11Z |
| format | Article |
| id | doaj.art-047a0e5849ce43e78ca4de0b3f155750 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-22T05:45:11Z |
| publishDate | 2017-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-047a0e5849ce43e78ca4de0b3f1557502022-12-21T18:37:03ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392017-01-0169350351210.2298/ABS160612126L0354-46641600126LAnatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seedsLazarević Jelena0Zorić Lana1Karagić Đura2Milošević Branko3Karanović Dunja4Milić Dubravka5Tepić Aleksandra6Luković Jadranka7Faculty of Sciences, Department of Biology and Ecology, Novi SadFaculty of Sciences, Department of Biology and Ecology, Novi SadInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadFaculty of Sciences, Department of Biology and Ecology, Novi SadFaculty of Sciences, Department of Biology and Ecology, Novi SadFaculty of Technology, Department of Food Preservation Engineering, Novi SadFaculty of Sciences, Department of Biology and Ecology, Novi SadIn this paper, we present the morphological characteristics of the seed and micromorphological, anatomical and chemical characteristics of the seed coat of pea (Pisum sativum L.) genotypes, Jezero, Javor and NS Junior. Our aim was to investigate whether these genotypes can be differentiated based on seed coat morphoanatomical characteristics, depending on the harvest treatment. The observations and measurements of seed coat cross-sections were performed using light microscopy. The seed coat surface was observed using SEM. A tuberculate seed coat surface characterized all examined pea genotypes, and the average diameter of the tubercle was about 12 μm. Statistical and laboratory analyses revealed that major damage was the most frequent defect type as the result of mechanized harvest in all the examined genotypes. Genotype NS Junior had the shortest seed length (6.1 mm). Micromorphological analysis revealed that the seed surface was tuberculate in all genotypes. The genotype Jezero had the highest number of tubercle ribs (11.0) and a significantly higher proportion of parenchyma tissue (50.6%), while NS Junior was characterized by the greatest share of macrosclereids (49.8%). The highest number of osteosclereids (832/mm2) was counted in genotype Javor. In addition, genotype NS Junior stands out due to the highest percentage of crude fiber (62.75 g/100g) in the seed coat. There was a marked difference among the studied genotypes with regard to the seed coat morphoanatomical characteristics, which is confirmed by the results of multivariate discriminant analysis (MDA). These results suggested that the morphological, micromorphological and anatomical characteristics of the seed might have an impact on the seed coat damage level at harvest. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 31024 and Grant no. 173002]http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600126L.pdfanatomyseed micromorphologyseed coat |
| spellingShingle | Lazarević Jelena Zorić Lana Karagić Đura Milošević Branko Karanović Dunja Milić Dubravka Tepić Aleksandra Luković Jadranka Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds Archives of Biological Sciences anatomy seed micromorphology seed coat |
| title | Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds |
| title_full | Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds |
| title_fullStr | Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds |
| title_full_unstemmed | Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds |
| title_short | Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds |
| title_sort | anatomical and micromorphological characteristics of the seed coat of field pea pisum sativum l genotypes in relation to cracks and damage of seeds |
| topic | anatomy seed micromorphology seed coat |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600126L.pdf |
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