Embryonic stem-like cells derived from in vitro produced bovine blastocysts
The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). T...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2011-06-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009 |
| _version_ | 1819160346586251264 |
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| author | Erika Regina Leal de Freitas Bruno Valente Sanches Maria Lúcia Gambarini Benedito Dias Oliveira Filho Lidia Andreu Guillo |
| author_facet | Erika Regina Leal de Freitas Bruno Valente Sanches Maria Lúcia Gambarini Benedito Dias Oliveira Filho Lidia Andreu Guillo |
| author_sort | Erika Regina Leal de Freitas |
| collection | DOAJ |
| description | The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts. |
| first_indexed | 2024-12-22T16:54:59Z |
| format | Article |
| id | doaj.art-047c3195f89b4535befd36a8b414f625 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-22T16:54:59Z |
| publishDate | 2011-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-047c3195f89b4535befd36a8b414f6252022-12-21T18:19:28ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242011-06-0154349550210.1590/S1516-89132011000300009Embryonic stem-like cells derived from in vitro produced bovine blastocystsErika Regina Leal de FreitasBruno Valente SanchesMaria Lúcia GambariniBenedito Dias Oliveira FilhoLidia Andreu GuilloThe aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009Bovine blastocystsICM isolationEmbryonic stem cellsGene markers |
| spellingShingle | Erika Regina Leal de Freitas Bruno Valente Sanches Maria Lúcia Gambarini Benedito Dias Oliveira Filho Lidia Andreu Guillo Embryonic stem-like cells derived from in vitro produced bovine blastocysts Brazilian Archives of Biology and Technology Bovine blastocysts ICM isolation Embryonic stem cells Gene markers |
| title | Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
| title_full | Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
| title_fullStr | Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
| title_full_unstemmed | Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
| title_short | Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
| title_sort | embryonic stem like cells derived from in vitro produced bovine blastocysts |
| topic | Bovine blastocysts ICM isolation Embryonic stem cells Gene markers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009 |
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