Frequencies of two CYP2C19 defective alleles (CYP2C19*2, and *3) among Iranian population in Mazandaran Province
Background: Cytochrome P450 2C19 (CYP2C19) is a polymorphically expressed enzyme that shows marked interindividual and interethnic variation. CYP2C19*2 and CYP2C19*3 are the most frequently identified defective alleles in Orientals and Caucasian poor metabolizers (PM). The aim of this study was to i...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences and Health Services
2013-02-01
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| Series: | Research in Molecular Medicine |
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| Online Access: | http://rmm.mazums.ac.ir/browse.php?a_code=A-10-326-1&slc_lang=en&sid=1 |
| _version_ | 1818408029534552064 |
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| author | Naghi Shahabi-Majd Mohammad bagher Hashemi-Sotehoh Baharak Habashi Mohammad reza Shiran |
| author_facet | Naghi Shahabi-Majd Mohammad bagher Hashemi-Sotehoh Baharak Habashi Mohammad reza Shiran |
| author_sort | Naghi Shahabi-Majd |
| collection | DOAJ |
| description | Background: Cytochrome P450 2C19 (CYP2C19) is a polymorphically expressed enzyme that shows marked interindividual and interethnic variation. CYP2C19*2 and CYP2C19*3 are the most frequently identified defective alleles in Orientals and Caucasian poor metabolizers (PM). The aim of this study was to investigate the frequencies of CYP2C19*1, CYP2C19*2 and CYP2C19*3 alleles and CYP2C19 genotypes in the Mazandarani ethnic group among Iranian Population.
Materials and Methods: The study was conducted in 103 unrelated healthy volunteers. DNA was extracted from leucocytes and analyzed by the PCR-RFLP protocol. The PCR product was digested with restriction enzymes (SmaI and BamH1) and then separated electrophoretically using polyacrylamide gel..
Results: Of the alleles tested, CYP2C19*1, and CYP2C19*2, but not CYP2C19*3, were detected. The frequencies for CYP2C19 alleles *1, *2, and *3 were 91%, 9.0%, and 0.0%, respectively. The frequencies of CYP2C19 genotypes *1/*1, *1/*2, *1/*3, *2/*2, *2/*3 and *3/*3 were 84%, 14%, 0.0%, 2.0%, and 0.0%, respectively.
Conclusion: The result of the present study showed that the two inactive alleles of CYP2C19 accounted for 9.0% of CYP2C19 alleles in our sample versus 8.8 - 40.1% reported in other populations. The frequencies of the studied alleles resulted significant differences between our sample and African and Eastern Asian populations. |
| first_indexed | 2024-12-14T09:37:14Z |
| format | Article |
| id | doaj.art-047df7048c9042c39f889e79d2824ad0 |
| institution | Directory Open Access Journal |
| issn | 2322-1348 2322-133X |
| language | English |
| last_indexed | 2024-12-14T09:37:14Z |
| publishDate | 2013-02-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-047df7048c9042c39f889e79d2824ad02022-12-21T23:07:53ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2013-02-01111721Frequencies of two CYP2C19 defective alleles (CYP2C19*2, and *3) among Iranian population in Mazandaran ProvinceNaghi Shahabi-Majd0Mohammad bagher Hashemi-Sotehoh1Baharak Habashi2Mohammad reza Shiran3 Molecular and Cell Biology Research Centre Molecular and Cell Biology Research Centre Psychiatry and Behavioral Sciences Research Centre Psychiatry and Behavioral Sciences Research Centre Background: Cytochrome P450 2C19 (CYP2C19) is a polymorphically expressed enzyme that shows marked interindividual and interethnic variation. CYP2C19*2 and CYP2C19*3 are the most frequently identified defective alleles in Orientals and Caucasian poor metabolizers (PM). The aim of this study was to investigate the frequencies of CYP2C19*1, CYP2C19*2 and CYP2C19*3 alleles and CYP2C19 genotypes in the Mazandarani ethnic group among Iranian Population. Materials and Methods: The study was conducted in 103 unrelated healthy volunteers. DNA was extracted from leucocytes and analyzed by the PCR-RFLP protocol. The PCR product was digested with restriction enzymes (SmaI and BamH1) and then separated electrophoretically using polyacrylamide gel.. Results: Of the alleles tested, CYP2C19*1, and CYP2C19*2, but not CYP2C19*3, were detected. The frequencies for CYP2C19 alleles *1, *2, and *3 were 91%, 9.0%, and 0.0%, respectively. The frequencies of CYP2C19 genotypes *1/*1, *1/*2, *1/*3, *2/*2, *2/*3 and *3/*3 were 84%, 14%, 0.0%, 2.0%, and 0.0%, respectively. Conclusion: The result of the present study showed that the two inactive alleles of CYP2C19 accounted for 9.0% of CYP2C19 alleles in our sample versus 8.8 - 40.1% reported in other populations. The frequencies of the studied alleles resulted significant differences between our sample and African and Eastern Asian populations.http://rmm.mazums.ac.ir/browse.php?a_code=A-10-326-1&slc_lang=en&sid=1CYP2C19 genotype alleles Mazandaran Iranian |
| spellingShingle | Naghi Shahabi-Majd Mohammad bagher Hashemi-Sotehoh Baharak Habashi Mohammad reza Shiran Frequencies of two CYP2C19 defective alleles (CYP2C19*2, and *3) among Iranian population in Mazandaran Province Research in Molecular Medicine CYP2C19 genotype alleles Mazandaran Iranian |
| title | Frequencies of two CYP2C19 defective alleles (CYP2C19*2, and *3) among Iranian population in Mazandaran Province |
| title_full | Frequencies of two CYP2C19 defective alleles (CYP2C19*2, and *3) among Iranian population in Mazandaran Province |
| title_fullStr | Frequencies of two CYP2C19 defective alleles (CYP2C19*2, and *3) among Iranian population in Mazandaran Province |
| title_full_unstemmed | Frequencies of two CYP2C19 defective alleles (CYP2C19*2, and *3) among Iranian population in Mazandaran Province |
| title_short | Frequencies of two CYP2C19 defective alleles (CYP2C19*2, and *3) among Iranian population in Mazandaran Province |
| title_sort | frequencies of two cyp2c19 defective alleles cyp2c19 2 and 3 among iranian population in mazandaran province |
| topic | CYP2C19 genotype alleles Mazandaran Iranian |
| url | http://rmm.mazums.ac.ir/browse.php?a_code=A-10-326-1&slc_lang=en&sid=1 |
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