| Summary: | This research reveals the improved performance of bioelectrodes made with amination-modified glucose oxidase (GOx-NH<sub>2</sub>) and carboxyl-functionalized mesoporous carbon (OMC-COOH). Results showed that when applied with 10 mM EDC amination, the functional groups of NH<sub>2</sub> were successfully added to GOx, according to the analysis of <sup>1</sup>H-NMR, elemental composition, and FTIR spectra. Moreover, after the aminated modification, increased enzyme immobilization (124.01 ± 1.49 mg GOx-NH<sub>2</sub>/g OMC-COOH; 2.77-fold increase) and enzyme activity (1.17-fold increase) were achieved, compared with those of non-modified GOx. Electrochemical analysis showed that aminated modification enhanced the peak current intensity of Nafion/GOx-NH<sub>2</sub>/OMC-COOH (1.32-fold increase), with increases in the charge transfer coefficient <i>α</i> (0.54), the apparent electron transfer rate constant <i>k<sub>s</sub></i> (2.54 s<sup>−1</sup>), and the surface coverage <i>Γ</i> (2.91 × 10<sup>−9</sup> mol·cm<sup>−2</sup>). Results showed that GOx-NH<sub>2</sub>/OMC-COOH exhibited impressive electro-activity and a favorable anodic reaction.
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