Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus
Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic expression in Brassica napus so far. Therefore, in the present study, global cytosin...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-10-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311914608388 |
| _version_ | 1818663081233874944 |
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| author | Fang WEI Jie HU Ming-zhu CUI Yan-hui ZHANG Yun-ling LI Bao-ming TIAN |
| author_facet | Fang WEI Jie HU Ming-zhu CUI Yan-hui ZHANG Yun-ling LI Bao-ming TIAN |
| author_sort | Fang WEI |
| collection | DOAJ |
| description | Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic expression in Brassica napus so far. Therefore, in the present study, global cytosine methylation and proteome were analysed in B. napus after cold treatment by methylation-sensitive amplified polymorphism (MSAP) and two-dimensional protein electrophoresis technology (2-DE). The results showed that the lowered genome-wide DNA methylation status was revealed after cold treatment, and about 0.88% of discrepancy in DNA methylation was detected between the non-flowering and flowering plants after cold treatment. Moreover, the 52 significantly up-regulated proteins emerged in comparison with the 36 down-regulated proteins, as well as the 14 proteins exclusively detected in the flowering plants. Intriguingly the 8 specifically expressed proteins in the non-flowering plants disappeared in the flowering plants with cold treatment. Therefore, these present data proved that the correlating changes of cytosine methylation and proteomic expression were evidenced under cold treatment in B. napus. |
| first_indexed | 2024-12-17T05:11:10Z |
| format | Article |
| id | doaj.art-047f5308868e4aa8b004870e9c42c4b8 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-17T05:11:10Z |
| publishDate | 2014-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-047f5308868e4aa8b004870e9c42c4b82022-12-21T22:02:15ZengElsevierJournal of Integrative Agriculture2095-31192014-10-01131021702176Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napusFang WEI0Jie HU1Ming-zhu CUI2Yan-hui ZHANG3Yun-ling LI4Bao-ming TIAN5School of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.China; Zhengzhou Key Laboratory of Plant Molecular Breeding, Zhengzhou 450001, P.R.China; WEI FangSchool of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.China; Zhengzhou Key Laboratory of Plant Molecular Breeding, Zhengzhou 450001, P.R.China; Correspondence TIAN Bao-ming, Tel/Fax: +86-371-67739513Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic expression in Brassica napus so far. Therefore, in the present study, global cytosine methylation and proteome were analysed in B. napus after cold treatment by methylation-sensitive amplified polymorphism (MSAP) and two-dimensional protein electrophoresis technology (2-DE). The results showed that the lowered genome-wide DNA methylation status was revealed after cold treatment, and about 0.88% of discrepancy in DNA methylation was detected between the non-flowering and flowering plants after cold treatment. Moreover, the 52 significantly up-regulated proteins emerged in comparison with the 36 down-regulated proteins, as well as the 14 proteins exclusively detected in the flowering plants. Intriguingly the 8 specifically expressed proteins in the non-flowering plants disappeared in the flowering plants with cold treatment. Therefore, these present data proved that the correlating changes of cytosine methylation and proteomic expression were evidenced under cold treatment in B. napus.http://www.sciencedirect.com/science/article/pii/S2095311914608388DNA methylation/demethylationMSAP2-DEcold treatmentBrassica napus |
| spellingShingle | Fang WEI Jie HU Ming-zhu CUI Yan-hui ZHANG Yun-ling LI Bao-ming TIAN Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus Journal of Integrative Agriculture DNA methylation/demethylation MSAP 2-DE cold treatment Brassica napus |
| title | Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus |
| title_full | Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus |
| title_fullStr | Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus |
| title_full_unstemmed | Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus |
| title_short | Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus |
| title_sort | global analysis of cytosine methylation and proteome under cold treatment in brassica napus |
| topic | DNA methylation/demethylation MSAP 2-DE cold treatment Brassica napus |
| url | http://www.sciencedirect.com/science/article/pii/S2095311914608388 |
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