Optimization and in-vivo evaluation of isradipine nanoparticles using Box-Behnken design surface response methodology
The isradipine is the potent anti hypertensive drug, which is matrix in polymeric nanoparticle by using solvent evaporation method. In this work, 3-factor, 3-level Box-Behnken design was used to optimize the process parameters like polymer concentration (A), sonication frequency (B) and sonication t...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | OpenNano |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352952015300086 |
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| author | Vijayan Venugopal K. Jayaraja Kumar S. Muralidharan S. Parasuraman P. Vasanth Raj K. Venkates Kumar |
| author_facet | Vijayan Venugopal K. Jayaraja Kumar S. Muralidharan S. Parasuraman P. Vasanth Raj K. Venkates Kumar |
| author_sort | Vijayan Venugopal |
| collection | DOAJ |
| description | The isradipine is the potent anti hypertensive drug, which is matrix in polymeric nanoparticle by using solvent evaporation method. In this work, 3-factor, 3-level Box-Behnken design was used to optimize the process parameters like polymer concentration (A), sonication frequency (B) and sonication time (C). Three dependent variable’s particle size, entrapment efficiency and practical yield were measured as responses. Mathematical equations and response surface plots were used to relate the dependent and independent variables. The optimization model of particle size of 343.14 nm, entrapment efficiency of about 83.74% and practical yield of 85.39% with A, B and C levels of 750 mg, 37.5 min and 40 kHz respectively. The observed responses were in close agreement with the predicted values of the optimized process. The prepared nanoparticle was characterized by Fourier transform infrared spectroscopy, morphological studies and in-vitro drug release studies. The prepared nanoparticle was showed good sustained release of drug upto 24 h. The anti-hypertensive study was performed on animal model. The PMMA (Poly-Methyl-Metha- Acrylate) isradipine nano particle shows fall in blood pressure was delayed and reach 152±2 mmHg at 1 h. The action was sustained until prolong period. Based on pharmacokinetic and pharmacodynamics parameter, the isradipine nanoparticles shows better bioavailability compare with solution form. |
| first_indexed | 2024-12-11T06:53:04Z |
| format | Article |
| id | doaj.art-047f72428af84fc6ab3b58afa029855d |
| institution | Directory Open Access Journal |
| issn | 2352-9520 |
| language | English |
| last_indexed | 2024-12-11T06:53:04Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | OpenNano |
| spelling | doaj.art-047f72428af84fc6ab3b58afa029855d2022-12-22T01:16:51ZengElsevierOpenNano2352-95202016-01-011C11510.1016/j.onano.2016.03.002Optimization and in-vivo evaluation of isradipine nanoparticles using Box-Behnken design surface response methodologyVijayan Venugopal0K. Jayaraja Kumar1S. Muralidharan2S. Parasuraman3P. Vasanth Raj4K. Venkates Kumar5Unit of Pharmaceutical Technology, Faculty of Pharmacy, AIMST University, MalaysiaUnit of Pharmaceutical Technology, Faculty of Pharmacy, AIMST University, MalaysiaUnit of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, MalaysiaUnit of Pharmacology, Faculty of Pharmacy, AIMST University, MalaysiaUnit of Pharmaceutical Technology, Faculty of Pharmacy, AIMST University, MalaysiaUnit of Pharmaceutical Technology, Faculty of Pharmacy, AIMST University, MalaysiaThe isradipine is the potent anti hypertensive drug, which is matrix in polymeric nanoparticle by using solvent evaporation method. In this work, 3-factor, 3-level Box-Behnken design was used to optimize the process parameters like polymer concentration (A), sonication frequency (B) and sonication time (C). Three dependent variable’s particle size, entrapment efficiency and practical yield were measured as responses. Mathematical equations and response surface plots were used to relate the dependent and independent variables. The optimization model of particle size of 343.14 nm, entrapment efficiency of about 83.74% and practical yield of 85.39% with A, B and C levels of 750 mg, 37.5 min and 40 kHz respectively. The observed responses were in close agreement with the predicted values of the optimized process. The prepared nanoparticle was characterized by Fourier transform infrared spectroscopy, morphological studies and in-vitro drug release studies. The prepared nanoparticle was showed good sustained release of drug upto 24 h. The anti-hypertensive study was performed on animal model. The PMMA (Poly-Methyl-Metha- Acrylate) isradipine nano particle shows fall in blood pressure was delayed and reach 152±2 mmHg at 1 h. The action was sustained until prolong period. Based on pharmacokinetic and pharmacodynamics parameter, the isradipine nanoparticles shows better bioavailability compare with solution form.http://www.sciencedirect.com/science/article/pii/S2352952015300086IsradipineNanoparticleSurface responseBox-Behnken designIn-vivo studies |
| spellingShingle | Vijayan Venugopal K. Jayaraja Kumar S. Muralidharan S. Parasuraman P. Vasanth Raj K. Venkates Kumar Optimization and in-vivo evaluation of isradipine nanoparticles using Box-Behnken design surface response methodology OpenNano Isradipine Nanoparticle Surface response Box-Behnken design In-vivo studies |
| title | Optimization and in-vivo evaluation of isradipine nanoparticles using Box-Behnken design surface response methodology |
| title_full | Optimization and in-vivo evaluation of isradipine nanoparticles using Box-Behnken design surface response methodology |
| title_fullStr | Optimization and in-vivo evaluation of isradipine nanoparticles using Box-Behnken design surface response methodology |
| title_full_unstemmed | Optimization and in-vivo evaluation of isradipine nanoparticles using Box-Behnken design surface response methodology |
| title_short | Optimization and in-vivo evaluation of isradipine nanoparticles using Box-Behnken design surface response methodology |
| title_sort | optimization and in vivo evaluation of isradipine nanoparticles using box behnken design surface response methodology |
| topic | Isradipine Nanoparticle Surface response Box-Behnken design In-vivo studies |
| url | http://www.sciencedirect.com/science/article/pii/S2352952015300086 |
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