RNA-programmed genome editing in human cells

RNA-programmed genome editing in human cells

Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA breaks (DSBs) at a site complementary to the guide...

Full description

Bibliographic Details
Main Authors: Martin Jinek, Alexandra East, Aaron Cheng, Steven Lin, Enbo Ma, Jennifer Doudna
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2013-01-01
Series:eLife
Subjects:
Cas9
endonuclease
genome editing
Online Access:https://elifesciences.org/articles/00471

Similar Items

Similar Items