Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against <i>Candida albicans</i> and <i>C. parapsilosis</i> Biofilms
<i>C. albicans</i> and <i>C. parapsilosis</i> are biofilm-forming yeasts responsible for bloodstream infections that can cause death. Synthetic antimicrobial peptides (SAMPs) are considered to be new weapons to combat these infections, alone or combined with drugs. Here, two...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/5/553 |
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| author | Leandro P. Bezerra Cleverson D. T. Freitas Ayrles F. B. Silva Jackson L. Amaral Nilton A. S. Neto Rafael G. G. Silva Aura L. C. Parra Gustavo H. Goldman Jose T. A. Oliveira Felipe P. Mesquita Pedro F. N. Souza |
| author_facet | Leandro P. Bezerra Cleverson D. T. Freitas Ayrles F. B. Silva Jackson L. Amaral Nilton A. S. Neto Rafael G. G. Silva Aura L. C. Parra Gustavo H. Goldman Jose T. A. Oliveira Felipe P. Mesquita Pedro F. N. Souza |
| author_sort | Leandro P. Bezerra |
| collection | DOAJ |
| description | <i>C. albicans</i> and <i>C. parapsilosis</i> are biofilm-forming yeasts responsible for bloodstream infections that can cause death. Synthetic antimicrobial peptides (SAMPs) are considered to be new weapons to combat these infections, alone or combined with drugs. Here, two SAMPs, called <i>Mo</i>-CBP<sub>3</sub>-PepI and <i>Mo</i>-CBP<sub>3</sub>-PepIII, were tested alone or combined with nystatin (NYS) and itraconazole (ITR) against <i>C. albicans</i> and <i>C. parapsilosis</i> biofilms. Furthermore, the mechanism of antibiofilm activity was evaluated by fluorescence and scanning electron microscopies. When combined with SAMPs, the results revealed a 2- to 4-fold improvement of NYS and ITR antibiofilm activity. Microscopic analyses showed cell membrane and wall damage and ROS overproduction, which caused leakage of internal content and cell death. Taken together, these results suggest the potential of <i>Mo</i>-CBP<sub>3</sub>-PepI and <i>Mo</i>-CBP<sub>3</sub>-PepIII as new drugs and adjuvants to increase the activity of conventional drugs for the treatment of clinical infections caused by <i>C. albicans</i> and <i>C. parapsilosis</i>. |
| first_indexed | 2024-03-10T03:27:55Z |
| format | Article |
| id | doaj.art-0487aa639fce47c78229b3eca71e07de |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:27:55Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-0487aa639fce47c78229b3eca71e07de2023-11-23T09:47:40ZengMDPI AGAntibiotics2079-63822022-04-0111555310.3390/antibiotics11050553Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against <i>Candida albicans</i> and <i>C. parapsilosis</i> BiofilmsLeandro P. Bezerra0Cleverson D. T. Freitas1Ayrles F. B. Silva2Jackson L. Amaral3Nilton A. S. Neto4Rafael G. G. Silva5Aura L. C. Parra6Gustavo H. Goldman7Jose T. A. Oliveira8Felipe P. Mesquita9Pedro F. N. Souza10Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451, CE, BrazilDepartment of Biology, Federal University of Ceará, Fortaleza 60451, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451, CE, BrazilFaculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, São Paulo P.O. Box 05508-000, SP, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451, CE, BrazilDrug Research and Development Center, Department of Physiology and Pharmacology, Federal University of Ceará, Rua Coronel, Nunes de Melo 100, Caixa, Fortaleza 60430-275, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451, CE, Brazil<i>C. albicans</i> and <i>C. parapsilosis</i> are biofilm-forming yeasts responsible for bloodstream infections that can cause death. Synthetic antimicrobial peptides (SAMPs) are considered to be new weapons to combat these infections, alone or combined with drugs. Here, two SAMPs, called <i>Mo</i>-CBP<sub>3</sub>-PepI and <i>Mo</i>-CBP<sub>3</sub>-PepIII, were tested alone or combined with nystatin (NYS) and itraconazole (ITR) against <i>C. albicans</i> and <i>C. parapsilosis</i> biofilms. Furthermore, the mechanism of antibiofilm activity was evaluated by fluorescence and scanning electron microscopies. When combined with SAMPs, the results revealed a 2- to 4-fold improvement of NYS and ITR antibiofilm activity. Microscopic analyses showed cell membrane and wall damage and ROS overproduction, which caused leakage of internal content and cell death. Taken together, these results suggest the potential of <i>Mo</i>-CBP<sub>3</sub>-PepI and <i>Mo</i>-CBP<sub>3</sub>-PepIII as new drugs and adjuvants to increase the activity of conventional drugs for the treatment of clinical infections caused by <i>C. albicans</i> and <i>C. parapsilosis</i>.https://www.mdpi.com/2079-6382/11/5/553antibiofilm activitycandidiasissynergismsynthetic peptidesantifungal drugs |
| spellingShingle | Leandro P. Bezerra Cleverson D. T. Freitas Ayrles F. B. Silva Jackson L. Amaral Nilton A. S. Neto Rafael G. G. Silva Aura L. C. Parra Gustavo H. Goldman Jose T. A. Oliveira Felipe P. Mesquita Pedro F. N. Souza Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against <i>Candida albicans</i> and <i>C. parapsilosis</i> Biofilms Antibiotics antibiofilm activity candidiasis synergism synthetic peptides antifungal drugs |
| title | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against <i>Candida albicans</i> and <i>C. parapsilosis</i> Biofilms |
| title_full | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against <i>Candida albicans</i> and <i>C. parapsilosis</i> Biofilms |
| title_fullStr | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against <i>Candida albicans</i> and <i>C. parapsilosis</i> Biofilms |
| title_full_unstemmed | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against <i>Candida albicans</i> and <i>C. parapsilosis</i> Biofilms |
| title_short | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against <i>Candida albicans</i> and <i>C. parapsilosis</i> Biofilms |
| title_sort | synergistic antifungal activity of synthetic peptides and antifungal drugs against i candida albicans i and i c parapsilosis i biofilms |
| topic | antibiofilm activity candidiasis synergism synthetic peptides antifungal drugs |
| url | https://www.mdpi.com/2079-6382/11/5/553 |
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