Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS<sub>2</sub> Nanosheets Decorated with Gold Nanoparticle Electrodes
Paper-based biosensors are considered simple and cost-efficient sensing platforms for analytical tests and diagnostics. Here, a paper-based electrochemical biosensor was developed for the rapid and sensitive detection of microRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. H...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/7/236 |
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| author | Hilal Torul Ece Yarali Ece Eksin Abhijit Ganguly John Benson Ugur Tamer Pagona Papakonstantinou Arzum Erdem |
| author_facet | Hilal Torul Ece Yarali Ece Eksin Abhijit Ganguly John Benson Ugur Tamer Pagona Papakonstantinou Arzum Erdem |
| author_sort | Hilal Torul |
| collection | DOAJ |
| description | Paper-based biosensors are considered simple and cost-efficient sensing platforms for analytical tests and diagnostics. Here, a paper-based electrochemical biosensor was developed for the rapid and sensitive detection of microRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. Hydrophobic barriers to creating electrode areas were manufactured by wax printing, whereas a three-electrode system was fabricated by a simple stencil approach. A carbon-based working electrode was modified using either reduced graphene oxide or molybdenum disulfide nanosheets modified with gold nanoparticle (AuNPs/RGO, AuNPs/MoS<sub>2</sub>) hybrid structures. The resulting paper-based biosensors offered sensitive detection of miRNA-155 and miRNA-21 by differential pulse voltammetry (DPV) in only 5.0 µL sample. The duration in our assay from the point of electrode modification to the final detection of miRNA was completed within only 35 min. The detection limits for miRNA-21 and miRNA-155 were found to be 12.0 and 25.7 nM for AuNPs/RGO and 51.6 and 59.6 nM for AuNPs/MoS<sub>2</sub> sensors in the case of perfectly matched probe-target hybrids. These biosensors were found to be selective enough to distinguish the target miRNA in the presence of single-base mismatch miRNA or noncomplementary miRNA sequences. |
| first_indexed | 2024-03-10T09:44:37Z |
| format | Article |
| id | doaj.art-0488d187f4d940279fce0c8d6e3b73f0 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T09:44:37Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-0488d187f4d940279fce0c8d6e3b73f02023-11-22T03:20:02ZengMDPI AGBiosensors2079-63742021-07-0111723610.3390/bios11070236Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS<sub>2</sub> Nanosheets Decorated with Gold Nanoparticle ElectrodesHilal Torul0Ece Yarali1Ece Eksin2Abhijit Ganguly3John Benson4Ugur Tamer5Pagona Papakonstantinou6Arzum Erdem7Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Ankara 06330, TurkeyDepartment of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova 35100, TurkeyDepartment of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova 35100, TurkeySchool of Engineering, Engineering Research Institute, Ulster University, Newtownabbey BT37 0QB, UK2-DTech, Core Technology Facility, 46 Grafton Street, Manchester M13 9NT, UKDepartment of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Ankara 06330, TurkeySchool of Engineering, Engineering Research Institute, Ulster University, Newtownabbey BT37 0QB, UKDepartment of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova 35100, TurkeyPaper-based biosensors are considered simple and cost-efficient sensing platforms for analytical tests and diagnostics. Here, a paper-based electrochemical biosensor was developed for the rapid and sensitive detection of microRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. Hydrophobic barriers to creating electrode areas were manufactured by wax printing, whereas a three-electrode system was fabricated by a simple stencil approach. A carbon-based working electrode was modified using either reduced graphene oxide or molybdenum disulfide nanosheets modified with gold nanoparticle (AuNPs/RGO, AuNPs/MoS<sub>2</sub>) hybrid structures. The resulting paper-based biosensors offered sensitive detection of miRNA-155 and miRNA-21 by differential pulse voltammetry (DPV) in only 5.0 µL sample. The duration in our assay from the point of electrode modification to the final detection of miRNA was completed within only 35 min. The detection limits for miRNA-21 and miRNA-155 were found to be 12.0 and 25.7 nM for AuNPs/RGO and 51.6 and 59.6 nM for AuNPs/MoS<sub>2</sub> sensors in the case of perfectly matched probe-target hybrids. These biosensors were found to be selective enough to distinguish the target miRNA in the presence of single-base mismatch miRNA or noncomplementary miRNA sequences.https://www.mdpi.com/2079-6374/11/7/236paper-based biosensorreduced graphene oxidemolybdenum disulfide nanosheetsmicroRNAgold nanohybridsdifferential pulse voltammetry |
| spellingShingle | Hilal Torul Ece Yarali Ece Eksin Abhijit Ganguly John Benson Ugur Tamer Pagona Papakonstantinou Arzum Erdem Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS<sub>2</sub> Nanosheets Decorated with Gold Nanoparticle Electrodes Biosensors paper-based biosensor reduced graphene oxide molybdenum disulfide nanosheets microRNA gold nanohybrids differential pulse voltammetry |
| title | Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS<sub>2</sub> Nanosheets Decorated with Gold Nanoparticle Electrodes |
| title_full | Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS<sub>2</sub> Nanosheets Decorated with Gold Nanoparticle Electrodes |
| title_fullStr | Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS<sub>2</sub> Nanosheets Decorated with Gold Nanoparticle Electrodes |
| title_full_unstemmed | Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS<sub>2</sub> Nanosheets Decorated with Gold Nanoparticle Electrodes |
| title_short | Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS<sub>2</sub> Nanosheets Decorated with Gold Nanoparticle Electrodes |
| title_sort | paper based electrochemical biosensors for voltammetric detection of mirna biomarkers using reduced graphene oxide or mos sub 2 sub nanosheets decorated with gold nanoparticle electrodes |
| topic | paper-based biosensor reduced graphene oxide molybdenum disulfide nanosheets microRNA gold nanohybrids differential pulse voltammetry |
| url | https://www.mdpi.com/2079-6374/11/7/236 |
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