An ace up their sleeve: a transcriptomic approach exposes the AceI efflux protein of Acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogens
The era of antibiotics as a cure-all for bacterial infections appears to be coming to an end. The emergence of multidrug resistance in many hospital-associated pathogens has resulted in superbugs that are effectively untreatable. Multidrug efflux pumps are well known mediators of bacterial drug resi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00333/full |
| _version_ | 1828435754494197760 |
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| author | Karl A Hassan Liam DH Elbourne Liping eLi Hasinika KA Hewawasam Gamage Qi eLiu Scott M Jackson David eSharples Anne-Brit eKolstø Peter JF Henderson Ian T Paulsen |
| author_facet | Karl A Hassan Liam DH Elbourne Liping eLi Hasinika KA Hewawasam Gamage Qi eLiu Scott M Jackson David eSharples Anne-Brit eKolstø Peter JF Henderson Ian T Paulsen |
| author_sort | Karl A Hassan |
| collection | DOAJ |
| description | The era of antibiotics as a cure-all for bacterial infections appears to be coming to an end. The emergence of multidrug resistance in many hospital-associated pathogens has resulted in superbugs that are effectively untreatable. Multidrug efflux pumps are well known mediators of bacterial drug resistance. Genome sequencing efforts have highlighted an abundance of putative efflux pump genes in bacteria. However, it is not clear how many of these pumps play a role in antimicrobial resistance. Several studies have demonstrated that efflux pump genes that participate in drug resistance are typically under tight regulatory control and expressed only in response to their substrates. Consequently, changes in gene expression following antimicrobial shock treatments may be used to identify efflux pumps that mediate antimicrobial resistance, informing targeted functional analyses of these proteins. Using this approach we have characterised novel efflux pumps in both Gram-negative and Gram-positive bacteria. Notably, we recently applied this strategy to characterise the AceI efflux pump from Acinetobacter. AceI is a prototype for a new family of multidrug efflux proteins that is conserved across many proteobacterial lineages. Different efflux pumps in this family have been shown to confer resistance to biocides including chlorhexidine, dequalinium, benzalkonium, proflavine and/or acriflavine. The discovery of this novel family of multidrug efflux proteins raises the possibility that additional undiscovered intrinsic resistance proteins may be encoded in the core genomes of pathogenic bacteria. |
| first_indexed | 2024-12-10T19:13:57Z |
| format | Article |
| id | doaj.art-0489472634e24edd85ad5e310fbaaadc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T19:13:57Z |
| publishDate | 2015-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0489472634e24edd85ad5e310fbaaadc2022-12-22T01:36:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-04-01610.3389/fmicb.2015.00333138007An ace up their sleeve: a transcriptomic approach exposes the AceI efflux protein of Acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogensKarl A Hassan0Liam DH Elbourne1Liping eLi2Hasinika KA Hewawasam Gamage3Qi eLiu4Scott M Jackson5David eSharples6Anne-Brit eKolstø7Peter JF Henderson8Ian T Paulsen9Macquarie UniversityMacquarie UniversityMacquarie UniversityMacquarie UniversityMacquarie UniversityUniversity of LeedsUniversity of LeedsUniversity of OsloUniversity of LeedsMacquarie UniversityThe era of antibiotics as a cure-all for bacterial infections appears to be coming to an end. The emergence of multidrug resistance in many hospital-associated pathogens has resulted in superbugs that are effectively untreatable. Multidrug efflux pumps are well known mediators of bacterial drug resistance. Genome sequencing efforts have highlighted an abundance of putative efflux pump genes in bacteria. However, it is not clear how many of these pumps play a role in antimicrobial resistance. Several studies have demonstrated that efflux pump genes that participate in drug resistance are typically under tight regulatory control and expressed only in response to their substrates. Consequently, changes in gene expression following antimicrobial shock treatments may be used to identify efflux pumps that mediate antimicrobial resistance, informing targeted functional analyses of these proteins. Using this approach we have characterised novel efflux pumps in both Gram-negative and Gram-positive bacteria. Notably, we recently applied this strategy to characterise the AceI efflux pump from Acinetobacter. AceI is a prototype for a new family of multidrug efflux proteins that is conserved across many proteobacterial lineages. Different efflux pumps in this family have been shown to confer resistance to biocides including chlorhexidine, dequalinium, benzalkonium, proflavine and/or acriflavine. The discovery of this novel family of multidrug efflux proteins raises the possibility that additional undiscovered intrinsic resistance proteins may be encoded in the core genomes of pathogenic bacteria.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00333/fullTranscriptomicsmultidrug efflux systemsBacterial drug resistanceAdaptive resistancebacterial transmembrane pair |
| spellingShingle | Karl A Hassan Liam DH Elbourne Liping eLi Hasinika KA Hewawasam Gamage Qi eLiu Scott M Jackson David eSharples Anne-Brit eKolstø Peter JF Henderson Ian T Paulsen An ace up their sleeve: a transcriptomic approach exposes the AceI efflux protein of Acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogens Frontiers in Microbiology Transcriptomics multidrug efflux systems Bacterial drug resistance Adaptive resistance bacterial transmembrane pair |
| title | An ace up their sleeve: a transcriptomic approach exposes the AceI efflux protein of Acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogens |
| title_full | An ace up their sleeve: a transcriptomic approach exposes the AceI efflux protein of Acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogens |
| title_fullStr | An ace up their sleeve: a transcriptomic approach exposes the AceI efflux protein of Acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogens |
| title_full_unstemmed | An ace up their sleeve: a transcriptomic approach exposes the AceI efflux protein of Acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogens |
| title_short | An ace up their sleeve: a transcriptomic approach exposes the AceI efflux protein of Acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogens |
| title_sort | ace up their sleeve a transcriptomic approach exposes the acei efflux protein of acinetobacter baumannii and reveals the drug efflux potential hidden in many microbial pathogens |
| topic | Transcriptomics multidrug efflux systems Bacterial drug resistance Adaptive resistance bacterial transmembrane pair |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00333/full |
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