Rab11a in the spinal cord: an essential contributor to complete Freund’s adjuvant-induced inflammatory pain in mice
Abstract Inflammatory pain is a commonly observed clinical symptom in a range of acute and chronic diseases. However, the mechanism of inflammatory pain is far from clear yet. Rab11a, a small molecule guanosine triphosphate enzyme, is reported to regulate orofacial inflammatory pain in our previous...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Molecular Brain |
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| Online Access: | https://doi.org/10.1186/s13041-023-01057-3 |
| _version_ | 1797555573704622080 |
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| author | Jun-Xiang Gu Jian Wang Fu-Juan Ma Miao-Miao Liu Si-Hai Chen Yi Wei Yi-Fan Xiao Pei-Yuan Lv Xin Liu Jian-Qiang Qu Xian-Xia Yan Tao Chen |
| author_facet | Jun-Xiang Gu Jian Wang Fu-Juan Ma Miao-Miao Liu Si-Hai Chen Yi Wei Yi-Fan Xiao Pei-Yuan Lv Xin Liu Jian-Qiang Qu Xian-Xia Yan Tao Chen |
| author_sort | Jun-Xiang Gu |
| collection | DOAJ |
| description | Abstract Inflammatory pain is a commonly observed clinical symptom in a range of acute and chronic diseases. However, the mechanism of inflammatory pain is far from clear yet. Rab11a, a small molecule guanosine triphosphate enzyme, is reported to regulate orofacial inflammatory pain in our previous works. However, the mechanism of Rab11a’s involvement in the regulation of inflammatory pain remains obscure. Here, we aim to elucidate the potential mechanisms through which Rab11a contributes to the development of inflammatory pain in the spinal level. It’s shown that neurons, rather than glial cells, were the primary cell type expressing Rab11a in the spinal dorsal horn (SDH). After intra-plantar injection of CFA, both the number of Fos/Rab11a-immunopositive neurons and the expression of Rab11a were increased. Administration of Rab11a-shRNA into the SDH resulted in significantly analgesic effect in mice with CFA injection. Application of Rab11a-shRNA also reduced the NMDA receptor-mediated excitatory post-synaptic current (EPSC) and the spike number of neurons in lamina II of the SDH in mice with CFA injection, without affecting the presynaptic glutamate release and the postsynaptic AMPA receptor-mediated EPSC. Our results thus suggest that the enhanced expression of neuronal Rab11a may be important for the process of inflammatory pain in mice with CFA injection, which is likely mediated by Rab11a’s potentiation of the competence of post-synaptic NMDAR and spiking of SDH neurons. |
| first_indexed | 2024-03-10T16:50:22Z |
| format | Article |
| id | doaj.art-048aeec7f26543ec96e8d2b295da16c6 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-03-10T16:50:22Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-048aeec7f26543ec96e8d2b295da16c62023-11-20T11:19:38ZengBMCMolecular Brain1756-66062023-09-0116111110.1186/s13041-023-01057-3Rab11a in the spinal cord: an essential contributor to complete Freund’s adjuvant-induced inflammatory pain in miceJun-Xiang Gu0Jian Wang1Fu-Juan Ma2Miao-Miao Liu3Si-Hai Chen4Yi Wei5Yi-Fan Xiao6Pei-Yuan Lv7Xin Liu8Jian-Qiang Qu9Xian-Xia Yan10Tao Chen11Department of Neurosurgery, the Second Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Anatomy and K.K. Leung Brain Research Centre, Fourth Military Medical UniversityDepartment of Anatomy and K.K. Leung Brain Research Centre, Fourth Military Medical UniversityDepartment of Respiratory and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Psychiatry, Xiaogan Mental Health CenterDepartment of Anatomy and K.K. Leung Brain Research Centre, Fourth Military Medical UniversityDepartment of Anatomy and K.K. Leung Brain Research Centre, Fourth Military Medical UniversityDepartment of Anatomy and K.K. Leung Brain Research Centre, Fourth Military Medical UniversityDepartment of Stomatology, The 960th Hospital of People’s Liberation ArmyDepartment of Neurosurgery, the Second Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Neurosurgery, the Second Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Neurosurgery, the Second Affiliated Hospital of Xi’an Jiaotong UniversityAbstract Inflammatory pain is a commonly observed clinical symptom in a range of acute and chronic diseases. However, the mechanism of inflammatory pain is far from clear yet. Rab11a, a small molecule guanosine triphosphate enzyme, is reported to regulate orofacial inflammatory pain in our previous works. However, the mechanism of Rab11a’s involvement in the regulation of inflammatory pain remains obscure. Here, we aim to elucidate the potential mechanisms through which Rab11a contributes to the development of inflammatory pain in the spinal level. It’s shown that neurons, rather than glial cells, were the primary cell type expressing Rab11a in the spinal dorsal horn (SDH). After intra-plantar injection of CFA, both the number of Fos/Rab11a-immunopositive neurons and the expression of Rab11a were increased. Administration of Rab11a-shRNA into the SDH resulted in significantly analgesic effect in mice with CFA injection. Application of Rab11a-shRNA also reduced the NMDA receptor-mediated excitatory post-synaptic current (EPSC) and the spike number of neurons in lamina II of the SDH in mice with CFA injection, without affecting the presynaptic glutamate release and the postsynaptic AMPA receptor-mediated EPSC. Our results thus suggest that the enhanced expression of neuronal Rab11a may be important for the process of inflammatory pain in mice with CFA injection, which is likely mediated by Rab11a’s potentiation of the competence of post-synaptic NMDAR and spiking of SDH neurons.https://doi.org/10.1186/s13041-023-01057-3Rab11aSpinal dorsal hornInflammatory painNMDA receptor |
| spellingShingle | Jun-Xiang Gu Jian Wang Fu-Juan Ma Miao-Miao Liu Si-Hai Chen Yi Wei Yi-Fan Xiao Pei-Yuan Lv Xin Liu Jian-Qiang Qu Xian-Xia Yan Tao Chen Rab11a in the spinal cord: an essential contributor to complete Freund’s adjuvant-induced inflammatory pain in mice Molecular Brain Rab11a Spinal dorsal horn Inflammatory pain NMDA receptor |
| title | Rab11a in the spinal cord: an essential contributor to complete Freund’s adjuvant-induced inflammatory pain in mice |
| title_full | Rab11a in the spinal cord: an essential contributor to complete Freund’s adjuvant-induced inflammatory pain in mice |
| title_fullStr | Rab11a in the spinal cord: an essential contributor to complete Freund’s adjuvant-induced inflammatory pain in mice |
| title_full_unstemmed | Rab11a in the spinal cord: an essential contributor to complete Freund’s adjuvant-induced inflammatory pain in mice |
| title_short | Rab11a in the spinal cord: an essential contributor to complete Freund’s adjuvant-induced inflammatory pain in mice |
| title_sort | rab11a in the spinal cord an essential contributor to complete freund s adjuvant induced inflammatory pain in mice |
| topic | Rab11a Spinal dorsal horn Inflammatory pain NMDA receptor |
| url | https://doi.org/10.1186/s13041-023-01057-3 |
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