<i>Mycobacterium chelonae</i> Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report
We present the case of a 72-year-old female patient with acute contained rupture of a biological composite graft, 21 months after replacement of the aortic valve and the ascending aorta due to an aortic dissection. Auramine-rhodamine staining of intraoperative biopsies showed acid-fast bacilli, but...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/381 |
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| author | Andrea C. Büchler Vladimir Lazarevic Nadia Gaïa Myriam Girard Friedrich Eckstein Adrian Egli Sarah Tschudin Sutter Jacques Schrenzel |
| author_facet | Andrea C. Büchler Vladimir Lazarevic Nadia Gaïa Myriam Girard Friedrich Eckstein Adrian Egli Sarah Tschudin Sutter Jacques Schrenzel |
| author_sort | Andrea C. Büchler |
| collection | DOAJ |
| description | We present the case of a 72-year-old female patient with acute contained rupture of a biological composite graft, 21 months after replacement of the aortic valve and the ascending aorta due to an aortic dissection. Auramine-rhodamine staining of intraoperative biopsies showed acid-fast bacilli, but classical culture and molecular methods failed to identify any organism. Metagenomic analysis indicated infection with <i>Mycobacterium</i> <i>chelonae</i>, which was confirmed by target-specific qPCR. The complexity of the sample required a customized bioinformatics pipeline, including cleaning steps to remove sequences of human, bovine ad pig origin. Our study underlines the importance of multiple testing to increase the likelihood of pathogen identification in highly complex samples. |
| first_indexed | 2024-03-10T03:38:13Z |
| format | Article |
| id | doaj.art-048b4377467442969610d25ee7c32a2d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:38:13Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-048b4377467442969610d25ee7c32a2d2023-11-23T11:39:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123138110.3390/ijms23010381<i>Mycobacterium chelonae</i> Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case ReportAndrea C. Büchler0Vladimir Lazarevic1Nadia Gaïa2Myriam Girard3Friedrich Eckstein4Adrian Egli5Sarah Tschudin Sutter6Jacques Schrenzel7Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, University of Basel, 4031 Basel, SwitzerlandGenomic Research Laboratory, Geneva University Hospitals, University of Geneva, 1206 Geneva, SwitzerlandGenomic Research Laboratory, Geneva University Hospitals, University of Geneva, 1206 Geneva, SwitzerlandGenomic Research Laboratory, Geneva University Hospitals, University of Geneva, 1206 Geneva, SwitzerlandDepartment of Cardiac Surgery, University Hospital Basel, 4031 Basel, SwitzerlandClinical Bacteriology and Mycology, University Hospital Basel, 4031 Basel, SwitzerlandDivision of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, University of Basel, 4031 Basel, SwitzerlandGenomic Research Laboratory, Geneva University Hospitals, University of Geneva, 1206 Geneva, SwitzerlandWe present the case of a 72-year-old female patient with acute contained rupture of a biological composite graft, 21 months after replacement of the aortic valve and the ascending aorta due to an aortic dissection. Auramine-rhodamine staining of intraoperative biopsies showed acid-fast bacilli, but classical culture and molecular methods failed to identify any organism. Metagenomic analysis indicated infection with <i>Mycobacterium</i> <i>chelonae</i>, which was confirmed by target-specific qPCR. The complexity of the sample required a customized bioinformatics pipeline, including cleaning steps to remove sequences of human, bovine ad pig origin. Our study underlines the importance of multiple testing to increase the likelihood of pathogen identification in highly complex samples.https://www.mdpi.com/1422-0067/23/1/381mycobacteria<i>Mycobacteroides</i>shotgun sequencingclinical metagenomicsaortic surgeryculture-negative infection |
| spellingShingle | Andrea C. Büchler Vladimir Lazarevic Nadia Gaïa Myriam Girard Friedrich Eckstein Adrian Egli Sarah Tschudin Sutter Jacques Schrenzel <i>Mycobacterium chelonae</i> Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report International Journal of Molecular Sciences mycobacteria <i>Mycobacteroides</i> shotgun sequencing clinical metagenomics aortic surgery culture-negative infection |
| title | <i>Mycobacterium chelonae</i> Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report |
| title_full | <i>Mycobacterium chelonae</i> Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report |
| title_fullStr | <i>Mycobacterium chelonae</i> Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report |
| title_full_unstemmed | <i>Mycobacterium chelonae</i> Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report |
| title_short | <i>Mycobacterium chelonae</i> Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report |
| title_sort | i mycobacterium chelonae i infection identified by metagenomic next generation sequencing as the probable cause of acute contained rupture of a biological composite graft a case report |
| topic | mycobacteria <i>Mycobacteroides</i> shotgun sequencing clinical metagenomics aortic surgery culture-negative infection |
| url | https://www.mdpi.com/1422-0067/23/1/381 |
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