Development, Validation, and Use of <sup>1</sup>H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid
Acerola (<i>Malpighia emarginata</i> D.C.) is an exotic fruit with high agro-industrial potential due to its high content of ascorbic acid (AA), phenolic compounds, and carotenoid pigments. Acerola fruit is processed into concentrated juice or powder to be incorporated into food suppleme...
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MDPI AG
2022-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/17/5614 |
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| author | Asma Bourafai-Aziez Daniel Jacob Gwladys Charpentier Emmanuel Cassin Guillaume Rousselot Annick Moing Catherine Deborde |
| author_facet | Asma Bourafai-Aziez Daniel Jacob Gwladys Charpentier Emmanuel Cassin Guillaume Rousselot Annick Moing Catherine Deborde |
| author_sort | Asma Bourafai-Aziez |
| collection | DOAJ |
| description | Acerola (<i>Malpighia emarginata</i> D.C.) is an exotic fruit with high agro-industrial potential due to its high content of ascorbic acid (AA), phenolic compounds, and carotenoid pigments. Acerola fruit is processed into concentrated juice or powder to be incorporated into food supplements. The ascorbic acid content of concentrated juice or powders must be controlled and well assessed. Therefore, the development of optimal methods and procedures for the rapid and accurate determination of the ascorbic acid content in juice concentrate and juice powder remains of considerable commercial interest. NMR spectroscopy is currently a powerful spectroscopic tool for the qualitative and quantitative analysis of molecules of all types and sizes. Firstly, this article presents the NMR-based metabolomic profiling of acerola juice and concentrate powder to describe and compare their composition. Thirty-six metabolites were identified. The AA over choline ratio and the NMR metabolomic profiles could be used for authentication in the future. Secondly, a rapid (8 min), reliable, and non-destructive method for the quantification of ascorbic acid by 1D <sup>1</sup>H-NMR spectroscopy was developed and validated. The LOD and LOQ were 0.05 and 0.15 mg/mL, respectively. These two approaches could be combined to better characterize ingredients derived from acerola and incorporated into food supplements. |
| first_indexed | 2024-03-10T01:28:20Z |
| format | Article |
| id | doaj.art-048f7530c25f4f42afe52ced08a50f8a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:28:20Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-048f7530c25f4f42afe52ced08a50f8a2023-11-23T13:45:21ZengMDPI AGMolecules1420-30492022-08-012717561410.3390/molecules27175614Development, Validation, and Use of <sup>1</sup>H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic AcidAsma Bourafai-Aziez0Daniel Jacob1Gwladys Charpentier2Emmanuel Cassin3Guillaume Rousselot4Annick Moing5Catherine Deborde6EVEAR EXTRACTION, 48 Route de Gennes, LD Félines, CEDEX 4, F-49320 Coutures, FranceINRAE, Univ. Bordeaux, Fruit Biology and Pathology, UMR1332, 71 Avenue E. Bourlaux, F-33140 Villenave d’Ornon, FranceEVEAR EXTRACTION, 48 Route de Gennes, LD Félines, CEDEX 4, F-49320 Coutures, FranceEVEAR EXTRACTION, 48 Route de Gennes, LD Félines, CEDEX 4, F-49320 Coutures, FranceEVEAR EXTRACTION, 48 Route de Gennes, LD Félines, CEDEX 4, F-49320 Coutures, FranceINRAE, Univ. Bordeaux, Fruit Biology and Pathology, UMR1332, 71 Avenue E. Bourlaux, F-33140 Villenave d’Ornon, FranceINRAE, Univ. Bordeaux, Fruit Biology and Pathology, UMR1332, 71 Avenue E. Bourlaux, F-33140 Villenave d’Ornon, FranceAcerola (<i>Malpighia emarginata</i> D.C.) is an exotic fruit with high agro-industrial potential due to its high content of ascorbic acid (AA), phenolic compounds, and carotenoid pigments. Acerola fruit is processed into concentrated juice or powder to be incorporated into food supplements. The ascorbic acid content of concentrated juice or powders must be controlled and well assessed. Therefore, the development of optimal methods and procedures for the rapid and accurate determination of the ascorbic acid content in juice concentrate and juice powder remains of considerable commercial interest. NMR spectroscopy is currently a powerful spectroscopic tool for the qualitative and quantitative analysis of molecules of all types and sizes. Firstly, this article presents the NMR-based metabolomic profiling of acerola juice and concentrate powder to describe and compare their composition. Thirty-six metabolites were identified. The AA over choline ratio and the NMR metabolomic profiles could be used for authentication in the future. Secondly, a rapid (8 min), reliable, and non-destructive method for the quantification of ascorbic acid by 1D <sup>1</sup>H-NMR spectroscopy was developed and validated. The LOD and LOQ were 0.05 and 0.15 mg/mL, respectively. These two approaches could be combined to better characterize ingredients derived from acerola and incorporated into food supplements.https://www.mdpi.com/1420-3049/27/17/5614acerolametabolomic profilingascorbic acidvitamin CqNMRfood supplement |
| spellingShingle | Asma Bourafai-Aziez Daniel Jacob Gwladys Charpentier Emmanuel Cassin Guillaume Rousselot Annick Moing Catherine Deborde Development, Validation, and Use of <sup>1</sup>H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid Molecules acerola metabolomic profiling ascorbic acid vitamin C qNMR food supplement |
| title | Development, Validation, and Use of <sup>1</sup>H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid |
| title_full | Development, Validation, and Use of <sup>1</sup>H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid |
| title_fullStr | Development, Validation, and Use of <sup>1</sup>H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid |
| title_full_unstemmed | Development, Validation, and Use of <sup>1</sup>H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid |
| title_short | Development, Validation, and Use of <sup>1</sup>H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid |
| title_sort | development validation and use of sup 1 sup h nmr spectroscopy for evaluating the quality of acerola based food supplements and quantifying ascorbic acid |
| topic | acerola metabolomic profiling ascorbic acid vitamin C qNMR food supplement |
| url | https://www.mdpi.com/1420-3049/27/17/5614 |
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