DETECTION OF DRUG ALLERGY ON ANTITUBERCULOSIS DRUGS USING LEUKOCYTES LYSIS SENSIBILIZATION REACTION AND TOTAL IMMUNOGLOBULIN E
Objective: Detecting the presence of drug allergy (DA) for anti-TB preparations on the basis of the studying of the immune mechanisms of DA in tuberculosis. Methods: Performed identification of the first and fourth types of DA for anti-tuberculosis drugs in 163 tuberculosis (TB) patients. Tubercu...
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| Format: | Article |
| Language: | English |
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Avicenna Tajik State Medical University
2018-06-01
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| Series: | Паёми Сино |
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| Online Access: | https://doi.org/10.25005/2074-0581-2018-20-2-3-207-211 |
| _version_ | 1797797239952769024 |
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| author | E.V. DUDENKO S. SYDYKOVA |
| author_facet | E.V. DUDENKO S. SYDYKOVA |
| author_sort | E.V. DUDENKO |
| collection | DOAJ |
| description | Objective: Detecting the presence of drug allergy (DA) for anti-TB preparations on the basis of the studying of the immune mechanisms of DA in tuberculosis.
Methods: Performed identification of the first and fourth types of DA for anti-tuberculosis drugs in 163 tuberculosis (TB) patients. Tuberculosis of the lungs (TBL) was diagnosed in 109 (66.9%) patients, of which 43 (39.4%) had multiple drug resistance (MDR) of Mycobacterium tuberculosis and 66 (60.6%) – drug sensitivity (DS). Extrapulmonary tuberculosis (EPLT) was detected in 54 (33.1%) of the 163 patients examined. We used a leukocyte lysis sensibilization reaction (LLSR) and quantified the concentration of total immunoglobulin of E class (IgE) by immunoferment analysis (IFA). The material of the study served as the samples of peripheral blood.
Results: Positive LLSR+ for one or more drugs was detected in 103 (63.2%) of the 163 patients with TB, weakly positive LLSR± in one or more drugs – in 43 (26.4%) and negative LLSR- for all studied drugs – in 17 (10.4%) patients with TB. The highest relative number of LLSR+ showed an examination in DA of MDR TBL patients (30 of 43 patients, or 69.8%). The greatest relative number of questionable (or weakly positive) results of LLSR was found in patients with EPLT (17 of 54 patients, or 31.5%). The highest relative number of negative results of the LLSR- was for the time being the examination in DA of patients with DS TBL (9 of 66, or 13.6%). In patients with DS and MDR TBL, the total IgE level was 140.6±52.3 and 147.0±70.1 IU/ml, respectively. The highest level of IgE (172.0±68.1 IU/ml) was detected in patients with EPLT.
Conclusion: Leukocytes reactions to incubation with anti-tuberculosis drugs (the fourth type of DA) was detected in 146 (89.6%) of patients with TB from 163 patients (LLSR+ and LLSR±), and the absence of this leukocyte reaction for all the drugs studied – in 17 (10.4%) of patients with TB from 163 surveyed with suspicion of DA (LLSR-). The highest level of IgE (172.0±68.1 IU/ml) was detected in patients with EPLT |
| first_indexed | 2024-03-13T03:45:11Z |
| format | Article |
| id | doaj.art-0490983992604e958acbf38e3bd8037f |
| institution | Directory Open Access Journal |
| issn | 2074-0581 2959-6327 |
| language | English |
| last_indexed | 2024-03-13T03:45:11Z |
| publishDate | 2018-06-01 |
| publisher | Avicenna Tajik State Medical University |
| record_format | Article |
| series | Паёми Сино |
| spelling | doaj.art-0490983992604e958acbf38e3bd8037f2023-06-22T21:15:10ZengAvicenna Tajik State Medical UniversityПаёми Сино2074-05812959-63272018-06-0120220721110.25005/2074-0581-2018-20-2-3-207-211DETECTION OF DRUG ALLERGY ON ANTITUBERCULOSIS DRUGS USING LEUKOCYTES LYSIS SENSIBILIZATION REACTION AND TOTAL IMMUNOGLOBULIN EE.V. DUDENKO0S. SYDYKOVA1Laboratory of Immunology and Molecular Biology, National Center for Phthisiology, Bishkek, Kyrgyz RepublicLaboratory of Immunology and Molecular Biology, National Center for Phthisiology, Bishkek, Kyrgyz RepublicObjective: Detecting the presence of drug allergy (DA) for anti-TB preparations on the basis of the studying of the immune mechanisms of DA in tuberculosis. Methods: Performed identification of the first and fourth types of DA for anti-tuberculosis drugs in 163 tuberculosis (TB) patients. Tuberculosis of the lungs (TBL) was diagnosed in 109 (66.9%) patients, of which 43 (39.4%) had multiple drug resistance (MDR) of Mycobacterium tuberculosis and 66 (60.6%) – drug sensitivity (DS). Extrapulmonary tuberculosis (EPLT) was detected in 54 (33.1%) of the 163 patients examined. We used a leukocyte lysis sensibilization reaction (LLSR) and quantified the concentration of total immunoglobulin of E class (IgE) by immunoferment analysis (IFA). The material of the study served as the samples of peripheral blood. Results: Positive LLSR+ for one or more drugs was detected in 103 (63.2%) of the 163 patients with TB, weakly positive LLSR± in one or more drugs – in 43 (26.4%) and negative LLSR- for all studied drugs – in 17 (10.4%) patients with TB. The highest relative number of LLSR+ showed an examination in DA of MDR TBL patients (30 of 43 patients, or 69.8%). The greatest relative number of questionable (or weakly positive) results of LLSR was found in patients with EPLT (17 of 54 patients, or 31.5%). The highest relative number of negative results of the LLSR- was for the time being the examination in DA of patients with DS TBL (9 of 66, or 13.6%). In patients with DS and MDR TBL, the total IgE level was 140.6±52.3 and 147.0±70.1 IU/ml, respectively. The highest level of IgE (172.0±68.1 IU/ml) was detected in patients with EPLT. Conclusion: Leukocytes reactions to incubation with anti-tuberculosis drugs (the fourth type of DA) was detected in 146 (89.6%) of patients with TB from 163 patients (LLSR+ and LLSR±), and the absence of this leukocyte reaction for all the drugs studied – in 17 (10.4%) of patients with TB from 163 surveyed with suspicion of DA (LLSR-). The highest level of IgE (172.0±68.1 IU/ml) was detected in patients with EPLThttps://doi.org/10.25005/2074-0581-2018-20-2-3-207-211tuberculosisdrug allergyanti-tuberculosis drugsleukocytes. |
| spellingShingle | E.V. DUDENKO S. SYDYKOVA DETECTION OF DRUG ALLERGY ON ANTITUBERCULOSIS DRUGS USING LEUKOCYTES LYSIS SENSIBILIZATION REACTION AND TOTAL IMMUNOGLOBULIN E Паёми Сино tuberculosis drug allergy anti-tuberculosis drugs leukocytes. |
| title | DETECTION OF DRUG ALLERGY ON ANTITUBERCULOSIS DRUGS USING LEUKOCYTES LYSIS SENSIBILIZATION REACTION AND TOTAL IMMUNOGLOBULIN E |
| title_full | DETECTION OF DRUG ALLERGY ON ANTITUBERCULOSIS DRUGS USING LEUKOCYTES LYSIS SENSIBILIZATION REACTION AND TOTAL IMMUNOGLOBULIN E |
| title_fullStr | DETECTION OF DRUG ALLERGY ON ANTITUBERCULOSIS DRUGS USING LEUKOCYTES LYSIS SENSIBILIZATION REACTION AND TOTAL IMMUNOGLOBULIN E |
| title_full_unstemmed | DETECTION OF DRUG ALLERGY ON ANTITUBERCULOSIS DRUGS USING LEUKOCYTES LYSIS SENSIBILIZATION REACTION AND TOTAL IMMUNOGLOBULIN E |
| title_short | DETECTION OF DRUG ALLERGY ON ANTITUBERCULOSIS DRUGS USING LEUKOCYTES LYSIS SENSIBILIZATION REACTION AND TOTAL IMMUNOGLOBULIN E |
| title_sort | detection of drug allergy on antituberculosis drugs using leukocytes lysis sensibilization reaction and total immunoglobulin e |
| topic | tuberculosis drug allergy anti-tuberculosis drugs leukocytes. |
| url | https://doi.org/10.25005/2074-0581-2018-20-2-3-207-211 |
| work_keys_str_mv | AT evdudenko detectionofdrugallergyonantituberculosisdrugsusingleukocyteslysissensibilizationreactionandtotalimmunoglobuline AT ssydykova detectionofdrugallergyonantituberculosisdrugsusingleukocyteslysissensibilizationreactionandtotalimmunoglobuline |