Effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish Pelteobagrus fulvidraco
The experiment was conducted to investigate the effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish. Five diets were formulated to contain five dietary leucine levels: 12.00 (control), 19.00, 26.00, 33.00, and 40.00 g kg−1. Each di...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1247410/full |
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| author | Dexiang Feng Yangping Yu Kaifang Liu Yi Su Tianyu Fan Xusheng Guo Ming Li |
| author_facet | Dexiang Feng Yangping Yu Kaifang Liu Yi Su Tianyu Fan Xusheng Guo Ming Li |
| author_sort | Dexiang Feng |
| collection | DOAJ |
| description | The experiment was conducted to investigate the effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish. Five diets were formulated to contain five dietary leucine levels: 12.00 (control), 19.00, 26.00, 33.00, and 40.00 g kg−1. Each diet was randomly assigned to triplicate groups of 30 juvenile fish (5.02 ± 0.15 g) twice daily to apparent satiation for 56 days. Weight gain rate, specific growth rate, and activities of liver superoxide dismutase, glutathione peroxidase, and serum lysozyme, as well as immunoglobulin M content, significantly increased with increase in dietary leucine levels up to 26.00 g kg−1, but those values decreased significantly with a further increase in dietary leucine. On the contrary, the lowest malondialdehyde content was found in 26.00 and 33.00 g kg−1 leucine groups. The expression levels of IGF 1 and MYF 5 genes in muscle were significantly upregulated with increase in dietary leucine levels up to 26.00 g kg−1, but the expression of MSTN level showed the opposite trend. The lowest expression levels of IL 8 and TNFɑ genes in the liver were found in 26.00 g kg−1 leucine groups. The quadratic regression analysis on weight gain, specific growth rate, and feed conversion ratio against dietary leucine levels indicated that the optimal dietary leucine requirement was estimated to be 26.84–27.00 g kg−1of the dry diet. |
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| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-12T21:46:51Z |
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| series | Frontiers in Physiology |
| spelling | doaj.art-049118a24fd7450c9ee1c72d8102a9622023-07-26T08:37:03ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-07-011410.3389/fphys.2023.12474101247410Effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish Pelteobagrus fulvidracoDexiang Feng0Yangping Yu1Kaifang Liu2Yi Su3Tianyu Fan4Xusheng Guo5Ming Li6School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, ChinaSchool of Marine Sciences, Ningbo University, Ningbo, ChinaSchool of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, ChinaSchool of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, ChinaSchool of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, ChinaSchool of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, ChinaSchool of Marine Sciences, Ningbo University, Ningbo, ChinaThe experiment was conducted to investigate the effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish. Five diets were formulated to contain five dietary leucine levels: 12.00 (control), 19.00, 26.00, 33.00, and 40.00 g kg−1. Each diet was randomly assigned to triplicate groups of 30 juvenile fish (5.02 ± 0.15 g) twice daily to apparent satiation for 56 days. Weight gain rate, specific growth rate, and activities of liver superoxide dismutase, glutathione peroxidase, and serum lysozyme, as well as immunoglobulin M content, significantly increased with increase in dietary leucine levels up to 26.00 g kg−1, but those values decreased significantly with a further increase in dietary leucine. On the contrary, the lowest malondialdehyde content was found in 26.00 and 33.00 g kg−1 leucine groups. The expression levels of IGF 1 and MYF 5 genes in muscle were significantly upregulated with increase in dietary leucine levels up to 26.00 g kg−1, but the expression of MSTN level showed the opposite trend. The lowest expression levels of IL 8 and TNFɑ genes in the liver were found in 26.00 g kg−1 leucine groups. The quadratic regression analysis on weight gain, specific growth rate, and feed conversion ratio against dietary leucine levels indicated that the optimal dietary leucine requirement was estimated to be 26.84–27.00 g kg−1of the dry diet.https://www.frontiersin.org/articles/10.3389/fphys.2023.1247410/fullleucinegrowthantioxidant enzymeimmune responsePelteobagrus fulvidraco |
| spellingShingle | Dexiang Feng Yangping Yu Kaifang Liu Yi Su Tianyu Fan Xusheng Guo Ming Li Effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish Pelteobagrus fulvidraco Frontiers in Physiology leucine growth antioxidant enzyme immune response Pelteobagrus fulvidraco |
| title | Effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish Pelteobagrus fulvidraco |
| title_full | Effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish Pelteobagrus fulvidraco |
| title_fullStr | Effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish Pelteobagrus fulvidraco |
| title_full_unstemmed | Effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish Pelteobagrus fulvidraco |
| title_short | Effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish Pelteobagrus fulvidraco |
| title_sort | effects of dietary leucine on growth antioxidant capacity immune response and inflammation in juvenile yellow catfish pelteobagrus fulvidraco |
| topic | leucine growth antioxidant enzyme immune response Pelteobagrus fulvidraco |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1247410/full |
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