Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast
Cargo traffic through the Golgi apparatus is mediated by cisternal maturation, but it remains largely unclear how the cis-cisternae, the earliest Golgi sub-compartment, is generated and how the Golgi matures into the trans-Golgi network (TGN). Here, we use high-speed and high-resolution confocal mic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2024-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/92900 |
| _version_ | 1827314517615312896 |
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| author | Takuro Tojima Yasuyuki Suda Natsuko Jin Kazuo Kurokawa Akihiko Nakano |
| author_facet | Takuro Tojima Yasuyuki Suda Natsuko Jin Kazuo Kurokawa Akihiko Nakano |
| author_sort | Takuro Tojima |
| collection | DOAJ |
| description | Cargo traffic through the Golgi apparatus is mediated by cisternal maturation, but it remains largely unclear how the cis-cisternae, the earliest Golgi sub-compartment, is generated and how the Golgi matures into the trans-Golgi network (TGN). Here, we use high-speed and high-resolution confocal microscopy to analyze the spatiotemporal dynamics of a diverse set of proteins that reside in and around the Golgi in budding yeast. We find many mobile punctate structures that harbor yeast counterparts of mammalian endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) proteins, which we term ‘yeast ERGIC’. It occasionally exhibits approach and contact behavior toward the ER exit sites and gradually matures into the cis-Golgi. Upon treatment with the Golgi-disrupting agent brefeldin A, the ERGIC proteins form larger aggregates corresponding to the Golgi entry core compartment in plants, while cis- and medial-Golgi proteins are absorbed into the ER. We further analyze the dynamics of several late Golgi proteins to better understand the Golgi-TGN transition. Together with our previous studies, we demonstrate a detailed spatiotemporal profile of the entire cisternal maturation process from the ERGIC to the Golgi and further to the TGN. |
| first_indexed | 2024-04-24T22:38:37Z |
| format | Article |
| id | doaj.art-04911ad0d3ce43839b254203620eb876 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-24T22:38:37Z |
| publishDate | 2024-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-04911ad0d3ce43839b254203620eb8762024-03-19T07:30:31ZengeLife Sciences Publications LtdeLife2050-084X2024-03-011310.7554/eLife.92900Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeastTakuro Tojima0https://orcid.org/0000-0002-9140-3205Yasuyuki Suda1https://orcid.org/0000-0001-8725-8001Natsuko Jin2https://orcid.org/0000-0002-0231-3057Kazuo Kurokawa3https://orcid.org/0000-0003-3549-4795Akihiko Nakano4https://orcid.org/0000-0003-3635-548XLive Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, JapanLive Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, Japan; Laboratory of Molecular Cell Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanLive Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, JapanLive Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, JapanLive Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, JapanCargo traffic through the Golgi apparatus is mediated by cisternal maturation, but it remains largely unclear how the cis-cisternae, the earliest Golgi sub-compartment, is generated and how the Golgi matures into the trans-Golgi network (TGN). Here, we use high-speed and high-resolution confocal microscopy to analyze the spatiotemporal dynamics of a diverse set of proteins that reside in and around the Golgi in budding yeast. We find many mobile punctate structures that harbor yeast counterparts of mammalian endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) proteins, which we term ‘yeast ERGIC’. It occasionally exhibits approach and contact behavior toward the ER exit sites and gradually matures into the cis-Golgi. Upon treatment with the Golgi-disrupting agent brefeldin A, the ERGIC proteins form larger aggregates corresponding to the Golgi entry core compartment in plants, while cis- and medial-Golgi proteins are absorbed into the ER. We further analyze the dynamics of several late Golgi proteins to better understand the Golgi-TGN transition. Together with our previous studies, we demonstrate a detailed spatiotemporal profile of the entire cisternal maturation process from the ERGIC to the Golgi and further to the TGN.https://elifesciences.org/articles/92900ERGICGECCOGolgi apparatusmembrane trafficcisternal maturationsuper-resolution live imaging |
| spellingShingle | Takuro Tojima Yasuyuki Suda Natsuko Jin Kazuo Kurokawa Akihiko Nakano Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast eLife ERGIC GECCO Golgi apparatus membrane traffic cisternal maturation super-resolution live imaging |
| title | Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast |
| title_full | Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast |
| title_fullStr | Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast |
| title_full_unstemmed | Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast |
| title_short | Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast |
| title_sort | spatiotemporal dissection of the golgi apparatus and the er golgi intermediate compartment in budding yeast |
| topic | ERGIC GECCO Golgi apparatus membrane traffic cisternal maturation super-resolution live imaging |
| url | https://elifesciences.org/articles/92900 |
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